| Field | Specification |
|---|---|
| Target | |
| Alternative names | Cadherin-1;CAM 120/80;Epithelial cadherin;E-cadherin;Uvomorulin;CD324;E-Cad/CTF1;E-Cad/CTF2;E-Cad/CTF3;CDH1;CDHE, UVO; |
| Gene ID | |
| UniProt # | |
| Reactivity | |
| Applications | |
| Immunogen | Expression system for standard: NS0; Immunogen sequence: D155-I707 |
| Expression system | |
| Expression region | |
| Assay type | |
| Sample type(s) | Cell Culture Supernatant, Serum, Plasma, Saliva, Urine |
| Sensitivity | |
| Detection range | |
| Assay time | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Assay Principle
This sandwich ELISA quantifies human cadherin-1 (gene CDH1) in cell culture supernatants, serum, plasma (heparin), saliva and urine. Strips are pre-coated with a capture antibody. After the sample is added, a biotinylated detection antibody binds the captured protein, and an avidin–biotin–peroxidase complex supplies the enzyme. TMB develops color in proportion to the bound target; the reaction is stopped and read at 450 nm, and concentrations are read from the standard curve.
E-cadherin (CDH1) is the calcium-dependent adhesion protein that builds adherens junctions between epithelial cells. Its cytoplasmic tail links to the actin cytoskeleton through catenins, and loss of E-cadherin is a hallmark of epithelial–mesenchymal transition and is well documented in diffuse gastric and lobular breast cancers.
Kit Components
| Anti-Human CDH1 Pre-coated 96-well strip microplate | 1 plate (96 wells) |
|---|---|
| Human CDH1 Standard | 2 vials, 10 ng/tube |
| Human CDH1 Biotinylated antibody (100x) | 100 µL |
| Avidin-Biotin-Peroxidase Complex (100x) | 100 µL |
| Sample Diluent | 30 mL |
| Antibody Diluent | 12 mL |
| Avidin-Biotin-Peroxidase Diluent | 12 mL |
| Color Developing Reagent (TMB) | 10 mL |
| Stop Solution | 10 mL |
| Wash Buffer (25x) | 20 mL |
| Adhesive plate sealers | 4 |
Also required (not supplied): microplate reader for 450 nm; incubator; automated plate washer (optional); precision pipettes for 0.5 µL through 1 mL volumes of aqueous solutions; multichannel pipettes (recommended for many samples); deionized or distilled water; 500 mL graduated cylinders; test tubes for dilution.
Kit components are not sold separately.
Performance Data
| Species | Human |
|---|---|
| Sample types | Cell culture supernatants, serum, plasma (heparin), saliva and urine |
| Assay range | 156 pg/mL–10,000 pg/mL |
| Sensitivity | <15 pg/mL |
| Standard | Recombinant human CDH1 (Asp155–Ile707), expressed in NS0 |
| Cross-reactivity | None detected against related proteins in supplier testing |
| Intra-assay CV | 4.6–6.8% (3 samples, n = 16 each) |
| Inter-assay CV | 4.9–6.8% (3 samples, n = 24 each) |
| Format | 96 wells, removable strips |
Sample dilution: start at 1:10. In the supplier’s tests, serum and plasma samples measured 17–71 ng/mL.
Example standard curve (OD450, pg/mL): 0 = 0.048; 156 = 0.086; 312 = 0.122; 625 = 0.192; 1250 = 0.325; 2500 = 0.547; 5000 = 1.043; 10000 = 1.884. Run a new standard curve on every plate.
Storage & Handling
Store at 4 °C for up to 6 months or −20 °C for up to 12 months. Avoid repeated freeze–thaw cycles. Ships on gel ice and tolerates up to 3 days at room temperature; freeze on arrival.
Safety
Sample data
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.048 | 0.086 | 0.122 | 0.192 | 0.325 | 0.547 | 1.043 | 1.884 |
Intra/inter assay consistency
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 265 | 1264 | 4789 | 253 | 1258 | 4691 |
| Standard deviation | 15.37 | 58.14 | 324.65 | 16.69 | 61.64 | 318.98 |
| CV (%) | 5.8% | 4.6% | 6.8% | 6.6% | 4.9% | 6.8% |
Kit components
Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4Materials required but not provided
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Can’t Find What You’re Looking For? We can help you source the best match or customize an ELISA solution for your study. Options may include alternative target synonyms, different species reactivity, sample type/matrix compatibility (serum/plasma/lysate/supernatant), assay format (sandwich/competitive), sensitivity/range, detection chemistry (colorimetric/fluorescent/chemiluminescent), plate format (pre-coated/uncoated, strips vs full plate), and bulk or custom packaging. Click Talk to a Scientist to submit a request form, email us at support@biohippo.com, or explore our Research Services for additional support. Our team will be in contact with you shortly.
Jin Peiyu et al. (2019) Long-term treatment with arsenite activates HER1 and HER2 through upregulating EGF, TGFα, and HSP90 in a human uroepithelial cell line. Cell Biology and Toxicology. 10.1007/s10565-019-09500-9
Yuan Jie et al. (2022) Systematic characterization of the components and molecular mechanisms of Jinshui Huanxian granules using UPLC-Orbitrap Fusion MS integrated with network pharmacology. Scientific Reports. 10.1038/s41598-022-16711-4
Igor Tsaur et al. (2019) sE-cadherin is upregulated in serum of patients with renal cell carcinoma and promotes tumor cell dissemination in vitro. Urologic Oncology-Seminars and Original Investigations. 10.1016/j.urolonc.2019.03.001
Weiwei She et al. (2023) Linc00511 Knockdown Inhibited TGF-β1-Induced Epithelial–Mesenchymal Transition of Bronchial Epithelial Cells by Targeting miR-16-5p/Smad3. American Journal of Rhinology & Allergy. 10.1177/19458924221144853
Liu Jieyu et al. (2017) sEcad and EGF Levels Increased in Urine of Non-ferrous Metal Workers and Medium of Uroepithelial Cell Line Treated by Arsenic. Biological Trace Element Research. 10.1007/s12011-017-1124-x
