| Field | Specification |
|---|---|
| Target | |
| Alternative names | DEL1; MGC26287; EGF-like repeats and discoidin I-like domains-containing protein 3|developmental endothelial locus-1 |
| UniProt # | |
| Species | |
| Applications | |
| Assay type | |
| Sensitivity | |
| Detection range | |
| Catalog no. (Mfr.) | |
| Main SKU |
Scientific Background
EDIL3 is a glycoprotein that is secreted by endothelial cells and can associate with the endothelial cell surface and the extracellular matrix. EDIL3 is regulated by hypoxia or vascular injury and has been implicated in vascular remodeling during angiogenesis.The predicted 480-amino acid mouse and human proteins, which are 95% identical, contain a signal peptide, 3 epidermal growth factor (EGF)-like repeats, and 2 discoidin I-like repeats. Northern blot analysis revealed expression of a 6.5-kb transcript in highly vascularized mouse embryonic day-15.5 tissue and in mouse and human endothelial cells, but not in other adult tissues. In situ hybridization analysis showed Del1 expression in developing endocardium at 9.5 days postcoitus and in renal artery and hypertrophic chondrocytes at 13.5 days postcoitus.
Assay Principle
This assay employs a two-site sandwich ELISA to quantitate EDIL3 in samples. An antibody specific for EDIL3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyEDIL3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for EDIL3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of EDIL3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Performance Data
| Assay Type | Sandwich ELISA |
|---|---|
| Detection Range | 0.312-20 ng/mL |
| Sensitivity | 0.127 ng/mL |
| Species Reactivity | Human (Homo sapiens) |
| Stability | The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C). |
| Manufacturer No. | AE61201HU |
| UniProt # | O43854 |
| Alternative Names | DEL1; MGC26287; EGF-like repeats and discoidin I-like domains-containing protein 3|developmental endothelial locus-1 |
Assay Workflow
The kit includes the components required for standard-curve quantification (see the kit manual for the component table and validated sample types). Absorbance is read on a standard microplate reader and concentrations are interpolated from the standard curve.
Safety & Handling
For research use only (RUO). Not for use in diagnostic or therapeutic procedures. Handle all samples and reagents using standard laboratory safety practices.
Related Products
Explore additional Human ELISA kits from Wuhan Abebio Science Co Ltd, or contact us for related assays for EDIL3 research.
Can’t Find What You’re Looking For? We can help you source the best match or customize an ELISA solution for your study. Options may include alternative target synonyms, different species reactivity, sample type/matrix compatibility (serum/plasma/lysate/supernatant), assay format (sandwich/competitive), sensitivity/range, detection chemistry (colorimetric/fluorescent/chemiluminescent), plate format (pre-coated/uncoated, strips vs full plate), and bulk or custom packaging. Click Talk to a Scientist to submit a request form, email us at support@biohippo.com, or explore our Research Services for additional support. Our team will be in contact with you shortly.
