| Field | Specification |
|---|---|
| Target | |
| Alternative names | Pro-epidermal growth factor; EGF |
| Gene ID | |
| UniProt # | |
| Reactivity | |
| Applications | |
| Immunogen | Expression system for standard: E.coli; Immunogen sequence: N971-R1023 |
| Expression system | |
| Expression region | |
| Assay type | |
| Sample type(s) | Cell Culture Supernatant, Serum, Plasma, Urine |
| Sensitivity | |
| Detection range | |
| Assay time | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Assay Principle
This single-incubation sandwich ELISA quantifies human pro-epidermal growth factor (gene EGF) in cell culture supernatants, serum, plasma (heparin, EDTA,citrate) and urine. The strips are coated with an anti-tag antibody. Samples and standards are added to the wells together with the kit’s antibody cocktail (Antibody A and Antibody B); the resulting antibody–target complex is held on the plate through the tag. After washing, TMB substrate is added and the HRP label on the detection antibody converts it to a colored product in proportion to the bound target. The reaction is stopped and the plate is read at 450 nm. The supplier markets the Quick format as a 90-minute assay.
Epidermal growth factor (EGF) is a small growth factor cut from a large membrane precursor. By binding and activating EGFR, it stimulates proliferation and survival of epithelial cells, and in the kidney it promotes magnesium reabsorption. It is widely used in cell culture and studied in development and cancer.
Kit Components
| Anti-tag Pre-coated 96-well Strip Microplate | 12 strips of 8 wells |
|---|---|
| Human EGF Standard | 2 × 1 ng/tube |
| Human EGF Antibody A | 3 mL |
| Human EGF Antibody B | 3 mL |
| Sample Diluent | 15 mL |
| TBS-T Wash Buffer (25x) | 12 mL |
| Color Developing Reagent (TMB) | 10 mL |
| Stop Solution | 10 mL |
| Adhesive Plate Sealers | 2 |
Also required (not supplied): microplate reader for 450 nm; automated plate washer (optional); precision pipettes for 0.5 µL through 1 mL volumes of aqueous solutions; multichannel pipettes (recommended for many samples); deionized or distilled water; 500 mL graduated cylinders; test tubes for dilution; horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3 mm.
Kit components are not sold separately.
Performance Data
| Species | Human |
|---|---|
| Sample types | Cell culture supernatants, serum, plasma (heparin, EDTA,citrate) and urine |
| Assay range | 4.7 pg/mL–300 pg/mL |
| Sensitivity | <1 pg/mL |
| Standard | Recombinant human EGF (Asn971–Arg1023), expressed in E. coli |
| Cross-reactivity | None detected against related proteins in supplier testing |
| Intra-assay CV | 5.7–7.4% (3 samples, n = 16 each) |
| Inter-assay CV | 6.6–9.1% (3 samples, n = 24 each) |
| Format | 96 wells, removable strips |
Example standard curve (OD450, pg/mL): 0 = 0.042; 4.7 = 0.106; 9.4 = 0.154; 18.8 = 0.254; 37.5 = 0.443; 75 = 0.773; 150 = 1.398; 300 = 2.199. Run a new standard curve on every plate.
Storage & Handling
Store at 4 °C for up to 6 months. Ships on gel ice and tolerates up to 3 days at room temperature; refrigerate on arrival.
Safety
Sample data
| Concentration (pg/ml) | 0 | 4.7 | 9.4 | 18.8 | 37.5 | 75 | 150 | 300 |
| O.D. | 0.042 | 0.106 | 0.154 | 0.254 | 0.443 | 0.773 | 1.398 | 2.199 |
Intra/inter assay consistency
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 12 | 79 | 131 | 10 | 84 | 135 |
| Standard deviation | 0.76 | 4.5 | 9.69 | 0.81 | 5.54 | 12.29 |
| CV (%) | 6.3% | 5.7% | 7.4% | 8.1% | 6.6% | 9.1% |
Kit components
Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x)|1|12ml Color Developing Reagent (TMB)|1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|PieceMaterials required but not provided
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional)
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
- Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.
Can’t Find What You’re Looking For? We can help you source the best match or customize an ELISA solution for your study. Options may include alternative target synonyms, different species reactivity, sample type/matrix compatibility (serum/plasma/lysate/supernatant), assay format (sandwich/competitive), sensitivity/range, detection chemistry (colorimetric/fluorescent/chemiluminescent), plate format (pre-coated/uncoated, strips vs full plate), and bulk or custom packaging. Click Talk to a Scientist to submit a request form, email us at support@biohippo.com, or explore our Research Services for additional support. Our team will be in contact with you shortly.
Dan Zhao et al. (2023) Histone Methyltransferase KMT2B Promotes Metastasis and Angiogenesis of Cervical Cancer by Upregulating EGF Expression. International Journal of Biological Sciences. 10.7150/ijbs.72381
Na Liu et al. (2023) Enhancing the paracrine effects of adipose stem cells using nanofiber-based meshes prepared by light-welding for accelerating wound healing. Materials & Design. 10.1016/j.matdes.2022.111582
Nie Xiongfeng et al. (2024) 3D printing sequentially strengthening high-strength natural polymer hydrogel bilayer scaffold for cornea regeneration. Regenerative Biomaterials. 10.1093/rb/rbae012
Zhang Jin et al. (2024) Adipose-derived stem cells derived decellularized extracellular matrix enabled skin regeneration and remodeling. Frontiers in Bioengineering and Biotechnology. 10.3389/fbioe.2024.1347995
Limin Bai et al. (2023) Impact of a Novel Hydrogel with Injectable Platelet-Rich Fibrin in Diabetic Wound Healing. Journal of Diabetes Research. 10.1155/2023/7532637
Md. Sohan et al. (2023) Association of reduced serum EGF and leptin levels with the pathophysiology of major depressive disorder: A case-control study. PLoS One. 10.1371/journal.pone.0288159
Hui Zhang et al. (2024) The Association Between Epidermal Growth Factor rs3756261 A/G Gene Polymorphism and the Risk of Ankylosing Spondylitis in a Chinese Han Population. International Journal of General Medicine. 10.2147/IJGM.S448976
