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Scientific Background
This ELISA kit applies to the in vitro quantitative determination of Human ESM1 concentrations in serum, plasma and other biological fluids.
Assay Principle
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human ESM1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ESM1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ESM1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human ESM1. You can calculate the concentration of Human ESM1 in the samples by comparing the OD of the samples to the standard curve.
Performance Specifications
| Sensitivity | 9.38 pg/mL |
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| Detection Range | 15.63-1000 pg/mL |
| Total Assay Time | 3 h 30 min |
| Compatible Sample Types | Serum, plasma and other biological fluids |
| Species Reactivity | Human |
| Detection Method | Sandwich |
| Precision (CV) | Both intra-CV and inter-CV are < 10%. |
| Recovery Rate | 80%-120% |
| Storage | 2-8℃,12 months |
✓ Research-Grade Validation
Specificity
This kit recognizes Human ESM1 in samples. No significant cross-reactivity or interference between Human ESM1 and analogues was observed
Safety & Regulatory
Handle reagents in accordance with institutional biosafety guidelines. Refer to the Safety Data Sheet (SDS) for complete hazard and handling information. Contains components that may require special disposal procedures per local regulations.
This kit is validated for use with Serum, plasma and other biological fluids. For unlisted matrices (e.g., tissue lysate, urine), perform a spike-and-recovery experiment to confirm assay performance before generating reportable data. Sample dilution in the kit's provided diluent is recommended to minimize matrix interference.
The minimum detectable concentration (sensitivity) of this kit is 9.38 pg/mL. Values below this threshold should be reported as below the limit of detection (<LOD) and should not be extrapolated from the standard curve.
The total assay time from sample addition to absorbance reading is approximately 3 h 30 min, including all incubation, wash, and substrate steps. Hands-on time is typically 1–2 hours; most steps involve passive plate incubation. Plan the assay as a single uninterrupted session for best results.
Standard components of this Sandwich ELISA Kit typically include: pre-coated microplate (96-well strip format), lyophilized or liquid recombinant ESM1 standard, detection antibody, streptavidin-HRP conjugate, TMB substrate, stop solution, wash buffer concentrate, and sample/standard diluent. Refer to the kit insert or datasheet for the exact component list and storage requirements.
This kit uses colorimetric (TMB/HRP) detection and requires a standard microplate absorbance reader capable of measuring at 450 nm. A reference wavelength of 570 nm or 630 nm is recommended to reduce background. No specialized fluorescence or luminescence reader is needed. Ensure the instrument is calibrated and the plate is clean and free of condensation before reading.
Tissue samples may contain endogenous or exogenous proteases during processing, leading to degradation of extracted proteins. Therefore, it's necessary to add protease inhibitors during processing to ensure the integrity of target proteins. If customers can keep samples cold and handle them quickly during processing, omitting the protease inhibitor may not have a significant effect. After preparation, samples should be tested promptly or immediately aliquoted and frozen at -20°C or -80°C.
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Endothelial Cell-Specific Molecule-1 (ESM1): An Endogenous Anticoagulant and Protective Factor in Venous Thrombosis (2026) Advanced Science. 10.1002/advs.202515994
Correlation of Lp(a), ApoB and oxLDL with Endothelial Damage Reading in Patients with Different Degrees of Coronary Atherosclerosis (2026) INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES. 10.3390/ijms27031160
Increased Carotid Intima–Media Thickness and Endothelial Cell-Specific Molecule-1 (Endocan) Levels in Obese Children (2021) ANGIOLOGY. 10.1177/0003319721995664
Endocan: A Novel Predictor of Endothelial Dysfunction in Silent Brain Infarction (2021) ANGIOLOGY. 10.1177/00033197211012135
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Effect of Non-Surgical Periodontal Treatment on Gingival Crevicular Fluid and Serum Endocan, Vascular Endothelial Growth Factor-A, and Tumor Necrosis Factor-Alpha Levels (2017) JOURNAL OF PERIODONTOLOGY. 10.1902/jop.2016.160279
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Maternal Serum Endocan Concentration in Pregnancies Complicated by Intrauterine Growth Restriction (2018) Reproductive Sciences. 10.1177/1933719118773480
The vasodilatory mechanism of nitric oxide and hydrogen sulfide in the human mesenteric artery in patients with colorectal cancer (2021) Experimental and Therapeutic Medicine. 10.3892/etm.2021.9646
The Evaluation of Serum Endocan, Interleukin-6, and CRP Levels Following Sleeve Gastrectomy (2023) International Journal of General Medicine. 10.2147/IJGM.S436213
Diagnostic Value of Serum and Urine Endocan Levels in Nonmuscle Invasive Bladder Cancer: A Prospective Comparative Study (2024) INTERNATIONAL JOURNAL OF CLINICAL PRACTICE. 10.1155/2024/2699706
The investigation of levels of endothelial cell-specific molecule, progranuline, clusterin, and human epididymis protein 4 in the differential diagnosis of malignant pleural effusions (2022) MEDICINE. 10.1097/MD.0000000000032471
The utility of maternal serum endocan level to predict preterm delivery within seven days in patients with threatened preterm labor (2019) Journal of Maternal-Fetal & Neonatal Medicine. 10.1080/14767058.2019.1649388
Serum endocan concentration and its correlation with severity of hypertensive disorders in pregnancy (2018) Journal of Maternal-Fetal & Neonatal Medicine. 10.1080/14767058.2018.1548597
Maternal serum endocan concentrations are elevated in patients with preterm premature rupture of membranes (2019) JOURNAL OF PERINATAL MEDICINE. 10.1515/jpm-2018-0401
The Relationship Between Endocan and Serum Inflammatory Markers in Patients with Senile Calcific Aortic Stenosis (2024) Anatolian Journal of Cardiology. 10.14744/AnatolJCardiol.2023.3695
Endocan may predict the presence of coronary slow flow and coronary artery disease (2024) Revista da Associacao Medica Brasileira. 10.1590/1806-9282.20240515
Endothelial cell-specific-molecule-1 (endocan) levels in women with premature ovarian insufficiency: a prospective comparative study (2020) JOURNAL OF OBSTETRICS AND GYNAECOLOGY. 10.1080/01443615.2020.1789952
Maternal serum endothelial cell-specific molecule-1 level and its correlation with severity of early-onset preeclampsia (2020) JOURNAL OF OBSTETRICS AND GYNAECOLOGY. 10.1080/01443615.2020.1819215
Does endocan level increase in women with polycystic ovary syndrome? A case — control study (2018) Ginekologia Polska. 10.5603/GP.a2018.0085
Serum Soluble Endocan Might Be a Predictor of Preterm Labor (2025) Annals of African Medicine. 10.4103/aam.aam_123_24
Is Serum Endocan Level an Indicator of the Severity of Childhood Community-Acquired Pneumonia? (2022) Journal of Pediatric Infectious Diseases. 10.1055/s-0042-1743576
The relationship of serum endocan levels and anti-TNF-alpha therapy in patients with ankylosing spondylitis (2018) European Journal of Rheumatology. 10.5152/eurjrheum.2017.17287
