| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | EA6; EAAT1; FLJ25094; GLAST; GLAST1; excitatory amino acid transporter 1|sodium-dependent glutamate/aspartate transporter |
| Product Type | |
| Sensitivity | |
| UniProt # |
Product Overview
Sandwich ELISA KitGlutamate and aspartate are excitatory neurotransmitters that have been implicated in a number of pathologic states of the nervous system. Accumulation of extracellular excitatory amino acids can be cytotoxic and may also lower the seizure threshold in epilepsy. EAAT1 (SLC1A3) is a member of a family of high-affinity sodium-dependent transporter molecules that regulate neurotransmitter concentrations at the excitatory glutamatergic synapses of the mammalian central nervous system. The cDNA encod…
Assay Principle
This assay employs a two-site sandwich ELISA to quantitate SLC1A3 in samples. An antibody specific for SLC1A3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySLC1A3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SLC1A3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound …
Performance Data
| Assay Type | Sandwich ELISA |
|---|---|
| Detection Method | Sandwich ELISA |
| Detection Range | 0.312-20 ng/mL |
Stability & Storage
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Bio…
Safety & Handling
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This is a sandwich ELISA kit. It employs a capture antibody pre-coated on the microplate surface and a biotin-conjugated detection antibody. After incubation with streptavidin-HRP and TMB substrate, a colorimetric signal proportional to analyte concentration is generated and measured at 450 nm.
The linear detection range of this kit is 0.312-20 ng/mL. Samples exceeding the upper limit must be diluted prior to testing. Samples below the lower limit may require concentration steps. Always verify that interpolated values fall within the linear portion of the standard curve.
This kit is validated for use with serum and plasma samples. Cell culture supernatants and tissue homogenates may also be tested after appropriate preparation. Avoid grossly hemolyzed, lipemic, or bacterially contaminated samples, as these may produce inaccurate results.
A microplate reader capable of measuring absorbance at 450 nm (with a 540–570 nm reference wavelength) is required. A plate washer is recommended for consistent washing steps. Calibrated micropipettes, incubator (37°C), and standard laboratory consumables are also needed.
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O… Prepare working reagents fresh for each run. Do not freeze kit components unless specifically directed in the insert.
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