Human Exfoliated Deciduous Teeth Stem Cells (SHED)

Overview

Human Exfoliated Deciduous Teeth Stem Cells (SHED) is a cell model used for research applications where physiologically relevant identity and donor background support interpretation of experimental readouts. Human Mesenchymal Stem Cells derived from Teeth (Exfoliated Deciduous Teeth Stem) within the Digestive system.

Human Exfoliated Deciduous Teeth Stem Cells (SHED) were identified to be a population of highly proliferative, clonogenic cells capable of differentiating into a variety of cell types including neural cells, adipocytes, and odontoblasts. After in vivo transplantation, SHED were found to be able to induce bone formation, generate dentin, and survive in mouse brain along with expression of neural markers. Figure 1. Human Exfoliated Deciduous Teeth Stem Cells (SHED) Osteogenic Induction (Day 24) iXCells Biotechnologies offers Human Exfoliated Deciduous Teeth Stem Cells (SHED)isolated from exfoliated deciduous teeth ofyoungdonors. These cells are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi. SHED are guaranteed to maintain at least 15 doublings in Exfoliated Deciduous Teeth Stem Cell Growth Medium (Cat# MD-0096)

Key elements and design rationale

• Cell identity: Mesenchymal Stem Cells (Primary Cells)
• Source context: Teeth; Exfoliated Deciduous Teeth Stem; Digestive
• Biosafety level: BSL-2 (follow your institution’s biosafety program and local regulations)

Product-specific elements (such as tissue source, donor background, and cell classification) help frame how results should be interpreted across assays and experimental conditions.

Biological background

Stem and progenitor cell models are widely used to study differentiation programs, lineage commitment, and regenerative responses under controlled culture perturbations.

Across primary and specialty cell models, experimental outcomes can be influenced by donor heterogeneity, passage history, confluence, and media composition. For interpretation, it is common to validate key markers or functional phenotypes in the user’s assay context and to document culture variables consistently.

Research relevance and current trends

• Increasing use of primary and specialty cells to improve translational relevance for target biology and phenotypic screening.
• Adoption of 3D culture formats and co-culture systems to better capture tissue microenvironments and cell–cell interactions.
• Integration of functional readouts with single-cell and multi-omics profiling to connect phenotype with molecular state.

Common research applications

• Profile identity markers by flow cytometry or immunostaining in cultured cells
• Induce lineage differentiation and track marker changes over a maturation time-course
• Quantify functional responses to defined stimuli relevant to the model system
• Compare baseline phenotype across donors/conditions using gene expression profiling
• Evaluate multipotency using lineage-specific staining and gene expression panels

Interpretation typically focuses on how a perturbation (e.g., cytokine exposure, metabolic stress, genetic manipulation, or compound treatment) shifts marker profiles or functional readouts relative to an appropriate control matched for donor and culture variables.

Notes for experimental interpretation

• Donor-to-donor heterogeneity can influence baseline phenotype and treatment response; include biological replicates when feasible.
• Passage number, confluence, and media composition can shift gene expression and functional readouts; track and report these variables consistently.
• Contamination control (including routine mycoplasma monitoring) supports reproducibility in downstream assays.
• Use appropriate negative/positive controls for the readout (e.g., unstimulated controls, pathway agonists/antagonists) to contextualize observed changes.

SKU:BHC18500073

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