| Field | Specification |
|---|---|
| Target | |
| Alternative names | Fatty acid-binding protein, intestinal;Fatty acid-binding protein 2;Intestinal-type fatty acid-binding protein;I-FABP;FABP2;FABPI; |
| Gene ID | |
| UniProt # | |
| Reactivity | |
| Applications | |
| Immunogen | Expression system for standard: E.coli; Immunogen sequence: A2-D132 |
| Expression system | |
| Expression region | |
| Assay type | |
| Sample type(s) | Cell Culture Supernatant, Cell Lysate, Serum, Plasma |
| Sensitivity | |
| Detection range | |
| Assay time | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Assay Principle
This sandwich ELISA quantifies human fatty acid-binding protein, intestinal (gene FABP2) in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Strips are pre-coated with a capture antibody. After the sample is added, a biotinylated detection antibody binds the captured protein, and an avidin–biotin–peroxidase complex supplies the enzyme. TMB develops color in proportion to the bound target; the reaction is stopped and read at 450 nm, and concentrations are read from the standard curve.
Intestinal fatty acid-binding protein (FABP2, I-FABP) is a small cytosolic protein in enterocytes of the small intestine. It binds long-chain fatty acids and is thought to support their uptake and packaging into triglyceride-rich lipoproteins. Because it leaks into blood when the gut lining is damaged, I-FABP is widely measured as a marker of intestinal epithelial injury.
Kit Components
| Anti-Human FABP2 Pre-coated 96-well strip microplate | 1 plate (96 wells) |
|---|---|
| Human FABP2 Standard | 2 vials, 10 ng/tube |
| Human FABP2 Biotinylated antibody (100x) | 100 µL |
| Avidin-Biotin-Peroxidase Complex (100x) | 100 µL |
| Sample Diluent | 30 mL |
| Antibody Diluent | 12 mL |
| Avidin-Biotin-Peroxidase Diluent | 12 mL |
| Color Developing Reagent (TMB) | 10 mL |
| Stop Solution | 10 mL |
| Wash Buffer (25x) | 20 mL |
| Adhesive plate sealers | 4 |
Also required (not supplied): microplate reader for 450 nm; incubator; automated plate washer (optional); precision pipettes for 0.5 µL through 1 mL volumes of aqueous solutions; multichannel pipettes (recommended for many samples); deionized or distilled water; 500 mL graduated cylinders; test tubes for dilution.
Kit components are not sold separately.
Performance Data
| Species | Human |
|---|---|
| Sample types | Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) |
| Assay range | 156 pg/mL-10000 pg/mL |
| Sensitivity | <10 pg/mL |
| Standard | Recombinant human FABP2 (Ala2–Asp132), expressed in E. coli |
| Cross-reactivity | None detected against related proteins in supplier testing |
| Intra-assay CV | 2.9–4.1% (3 samples, n = 16 each) |
| Inter-assay CV | 6.1–7.6% (3 samples, n = 24 each) |
| Format | 96 wells, removable strips |
Sample dilution: start at 1:10. In the supplier’s tests, serum and plasma samples measured 1–10 ng/mL.
Example standard curve (OD450, pg/mL): 0 = 0.0677; 156 = 0.1153; 312 = 0.1649; 625 = 0.2551; 1250 = 0.4243; 2500 = 0.7719; 5000 = 1.474; 10000 = 2.514. Run a new standard curve on every plate.
Storage & Handling
Store at 4 °C for up to 6 months or −20 °C for up to 12 months. Avoid repeated freeze–thaw cycles. Ships on gel ice and tolerates up to 3 days at room temperature; freeze on arrival.
Safety
Sample data
| Concentration (pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
| O.D. | 0.075 | 0.154 | 0.215 | 0.31 | 0.462 | 0.77 | 1.192 | 1.837 |
Intra/inter assay consistency
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 49 | 270 | 1038 | 51 | 272 | 977 |
| Standard deviation | 2.69 | 14.31 | 61.24 | 3.57 | 18.76 | 74.25 |
| CV (%) | 5.5% | 5.3% | 5.9% | 7% | 6.9% | 7.6% |
Kit components
Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4Materials required but not provided
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Yuxin Zhang et al. (2025) Bile acid receptor FXR promotes intestinal epithelial ferroptosis and subsequent ILC3 dysfunction in neonatal necrotizing enterocolitis. Immunity. 10.1016/j.immuni.2025.02.003
Serrano-Villar Sergio et al. (2021) Fecal microbiota transplantation in HIV: A pilot placebo-controlled study. Nature Communications. 10.1038/s41467-021-21472-1
María José Gosalbes et al. (2022) Interactions among the mycobiome, bacteriome, inflammation, and diet in people living with HIV. Gut Microbes. 10.1080/19490976.2022.2089002
Mónica Manzano et al. (2022) Relationship of Diet to Gut Microbiota and Inflammatory Biomarkers in People with HIV. Nutrients. 10.3390/nu14061221
Laura Vilà-Quintana et al. (2024) Exploring the Associations of Inflammatory and Oxidative Stress Biomarkers with Pancreatic Diseases: An Observational and Mendelian Randomisation Study. Journal of Clinical Medicine. 10.3390/jcm13082247
Jie-Hao Yuan et al. (2020) Impaired intestinal barrier function in type 2 diabetic patients measured by serum LPS, Zonulin, and IFABP. Journal of Diabetes and Its Complications. 10.1016/j.jdiacomp.2020.107766
Analuz Fernandez et al. (2026) Inflammatory Biomarkers Decay After First-Line Antiretroviral Therapy Initiation With Dolutegravir/Lamivudine or Bictegravir/Emtricitabine/Tenofovir Alafenamide in Persons With HIV: A Substudy of a Randomized Clinical Trial. Health Science Reports. 10.1002/hsr2.71584
