| Field | Specification |
|---|---|
| Species | |
| Applications | |
| Cell type | |
| Classification | |
| Tissue | |
| Disease | Normal |
| Biosafety level | |
| Storage | |
| Shipping | |
| Catalog no. (Mfr.) | |
| Main SKU |
Overview
Human Gastric Fibroblasts are isolated from stomach tissue of normal, healthy donors. Fibroblasts are responsible for secretion of the extracellular matrix in connective tissue and contribute to ulcer wound healing; gastric fibroblast activity has also been implicated in the development of gastric carcinoma, which makes these cells a practical model for both tissue-repair and tumour-microenvironment work.
Specifications
| Species | Human (Homo sapiens) |
|---|---|
| Tissue | Stomach (gastric) |
| Donor Status | Normal, healthy donors |
| Cell Type | Fibroblasts |
| Product Format | Cryopreserved |
| Pack Size | 0.5 million cells/vial |
| Passage at Cryopreservation | P1 |
| Growth Properties | Adherent |
| Recommended Medium | iXCells Fibroblast Growth Medium (MD-0011) |
| Expansion Capacity | No more than 3 passages |
| Storage | Liquid nitrogen |
Handling
| Thawing | Immerse the vial in a 37 °C water bath for 1-2 minutes with gentle agitation, transfer into 5 mL growth medium, centrifuge at 1,000 rpm for 5 minutes |
|---|---|
| Subculture | At 80-90% confluence treat with 0.25% Trypsin-EDTA for 3-5 minutes |
| Reseeding Density | 5,000 cells/cm2 |
Quality Control
- Identity verified by immunofluorescence staining for fibronectin and vimentin.
- Tested negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi.
Common Research Applications
- Gastric ulcer and wound-healing models.
- Extracellular matrix deposition and remodelling assays.
- Tumour-microenvironment co-culture with gastric carcinoma lines.
- Fibrosis and myofibroblast differentiation studies.
Notes for Experimental Interpretation
- The vendor limits expansion to no more than 3 passages; plan experiments within that window and record passage number with every readout.
- Use the recommended growth medium - fibroblast phenotype and matrix output shift with serum and supplement composition.
- Thaw rapidly and plate immediately; primary fibroblasts lose viability quickly at intermediate temperatures.
Safety & Intended Use
Intended UseResearch Use Only (RUO)VendoriXCells BiotechnologiesCatalog No.10HU-239
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