| Field | Specification |
|---|---|
| Target | |
| Alternative names | Glial cell line-derived neurotrophic factor;hGDNF;Astrocyte-derived trophic factor;ATF;GDNF; |
| Gene ID | |
| UniProt # | |
| Reactivity | |
| Applications | |
| Immunogen | Expression system for standard: NS0; Immunogen sequence: R109-I211 |
| Expression system | |
| Expression region | |
| Assay type | |
| Sample type(s) | Cell Culture Supernatant, Cell Lysate, Serum, Plasma |
| Sensitivity | |
| Detection range | |
| Assay time | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Assay Principle
This sandwich ELISA quantifies human glial cell line-derived neurotrophic factor (gene GDNF) in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Strips are pre-coated with a capture antibody. After the sample is added, a biotinylated detection antibody binds the captured protein, and an avidin–biotin–peroxidase complex supplies the enzyme. TMB develops color in proportion to the bound target; the reaction is stopped and read at 450 nm, and concentrations are read from the standard curve.
Glial cell line-derived neurotrophic factor (GDNF) is a secreted growth factor that strongly supports the survival of dopaminergic and motor neurons. It binds the coreceptor GFRα1 and signals through the RET tyrosine kinase. GDNF is also required for kidney development and spermatogonial stem cell maintenance, and it is studied in Parkinson's disease research.
Kit Components
| Anti-Human GDNF Pre-coated 96-well strip microplate | 1 plate (96 wells) |
|---|---|
| Human GDNF Standard | 2 vials, 10 ng/tube |
| Human GDNF Biotinylated antibody (100x) | 100 µL |
| Avidin-Biotin-Peroxidase Complex (100x) | 100 µL |
| Sample Diluent | 30 mL |
| Antibody Diluent | 12 mL |
| Avidin-Biotin-Peroxidase Diluent | 12 mL |
| Color Developing Reagent (TMB) | 10 mL |
| Stop Solution | 10 mL |
| Wash Buffer (25x) | 20 mL |
| Adhesive plate sealers | 4 |
Also required (not supplied): microplate reader for 450 nm; incubator; automated plate washer (optional); precision pipettes for 0.5 µL through 1 mL volumes of aqueous solutions; multichannel pipettes (recommended for many samples); deionized or distilled water; 500 mL graduated cylinders; test tubes for dilution.
Kit components are not sold separately.
Performance Data
| Species | Human |
|---|---|
| Sample types | Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) |
| Assay range | 31.2 pg/mL–2,000 pg/mL |
| Sensitivity | <4 pg/mL |
| Standard | Recombinant human GDNF (Arg109–Ile211), expressed in NS0 |
| Cross-reactivity | None detected against related proteins in supplier testing |
| Intra-assay CV | 4.3–7.2% (3 samples, n = 16 each) |
| Inter-assay CV | 4.6–9.1% (3 samples, n = 24 each) |
| Format | 96 wells, removable strips |
Example standard curve (OD450, pg/mL): 0 = 0.03; 31.2 = 0.081; 62.5 = 0.123; 125 = 0.212; 250 = 0.336; 500 = 0.609; 1000 = 1.031; 2000 = 1.798. Run a new standard curve on every plate.
Storage & Handling
Store at 4 °C for up to 6 months or −20 °C for up to 12 months. Avoid repeated freeze–thaw cycles. Ships on gel ice and tolerates up to 3 days at room temperature; freeze on arrival.
Safety
Sample data
| Concentration (pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
| O.D. | 0.03 | 0.081 | 0.123 | 0.212 | 0.336 | 0.609 | 1.031 | 1.798 |
Intra/inter assay consistency
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 69 | 264 | 855 | 66 | 242 | 810 |
| Standard deviation | 4.96 | 17.16 | 36.76 | 6 | 16.45 | 37.26 |
| CV (%) | 7.2% | 6.5% | 4.3% | 9.1% | 6.8% | 4.6% |
Kit components
Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4Materials required but not provided
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Ozkan Serbay et al. (2022) Therapeutic potential of conditioned medium obtained from deferoxamine preconditioned umbilical cord mesenchymal stem cells on diabetic nephropathy model. Stem Cell Research & Therapy. 10.1186/s13287-022-03121-6
Jian Du et al. (2023) Improving Schwann Cell Differentiation from Human Adipose Stem Cells with Metabolic Glycoengineering. Cells. 10.3390/cells12081190
Michelle D. Drewry et al. (2022) Promoting and Orienting Axon Extension Using Scaffold-Free Dental Pulp Stem Cell Sheets. ACS Biomaterials Science & Engineering. 10.1021/acsbiomaterials.1c01517
Bowen Niu et al. (2016) Melatonin promotes goat spermatogonia stem cells (SSCs) proliferation by stimulating glial cell line-derived neurotrophic factor (GDNF) production in Sertoli cells. Oncotarget. 10.18632/oncotarget.12720
Qijing Lei et al. (2018) H19 regulates the proliferation of bovine male germline stem cells via IGF-1 signaling pathway. Journal of Cellular Physiology. 10.1002/jcp.26920
Meer N. Ahmed et al. (2021) Dental Pulp Cell Sheets Enhance Facial Nerve Regeneration via Local Neurotrophic Factor Delivery. Tissue Engineering Part A. 10.1089/ten.tea.2020.0265
Xue Li et al. (2020) A synergistic effect between family intervention and rTMS improves cognitive and negative symptoms in schizophrenia: A randomized controlled trial. Journal of Psychiatric Research. 10.1016/j.jpsychires.2020.04.009
Xiaoquan Wang et al. (2011) Association study between plasma GDNF and cognitive function in late-onset depression. Journal of Affective Disorders. 10.1016/j.jad.2011.03.043
Michelle D Drewry et al. (2024) Enhancing facial nerve regeneration with scaffold-free conduits engineered using dental pulp stem cells and their endogenous, aligned extracellular matrix. Journal of Neural Engineering. 10.1088/1741-2552/ad749d
Bilgiç Ayhan et al. (2016) Serum brain-derived neurotrophic factor, glial-derived neurotrophic factor, nerve growth factor, and neurotrophin-3 levels in children with attention-deficit/hyperactivity disorder. European Child & Adolescent Psychiatry. 10.1007/s00787-016-0898-2
