| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | GHBP;GHIP |
| Conjugate | |
| Formulation | |
| Molecular Weight | |
| Product Type | |
| Purity | |
| Reconstitution | |
| Shipping | |
| Storage | |
| Target | |
| UniProt # |
Scientific Background
This gene encodes a member of the type I cytokine receptor family, which is a transmembrane receptor for growth hormone. Binding of growth hormone to the receptor leads to receptor dimerization and the activation of an intra- and intercellular signal transduction pathway leading to growth. Mutations in this gene have been associated with Laron syndrome, also known as the growth hormone insensitivity syndrome (GHIS), a disorder characterized by short stature. In humans and rabbits, but not rodents, growth hormone binding protein (GHBP) is generated by proteolytic cleavage of the extracellular ligand-binding domain from the mature growth hormone receptor protein.
Product Description
Mammalian (HEK293) Expression | Recombinant ProteinRecombinant GHR Human protein is produced in Mammalian (HEK293) expression system and supplied as lyophilized powder for stable long-term storage. Suitable for binding assays, SPR/BLI interaction studies, antibody development, ELISA development, and drug discovery applications.
Protein Specifications
| UniProt ID | P10912 |
|---|---|
| Expression System | Mammalian (HEK293) |
| Molecular Characterization | GHR(Ala27-Tyr264) hFc(Glu99-Ala330) |
| Amino Acids / Region | Ala27-Tyr264 |
| Affinity Tag | C-Human Fc Tag |
| Molecular Weight | 53.8 kDa |
| Purity | The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. |
| Formulation | Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyo… |
| Storage | Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for… |
| Delivery | In Stock |
Safety & Handling
Research use only
This protein was produced in Mammalian (HEK293). Mammalian expression enables human-like glycosylation, correct disulfide bond formation, and proper protein folding — all critical for proteins used in binding interaction studies (SPR/BLI) and antibody characterisation. For transmembrane proteins expressed as ectodomains or nanodisc preparations, the expression system directly affects binding conformation and assay performance.
The molecular characterisation of this construct is: GHR(Ala27-Tyr264) hFc(Glu99-Ala330). Cross-reference with the UniProt entry (P10912) to confirm the expressed region includes your domain of interest — receptor-binding domain, extracellular domain, or specific epitope — before ordering.
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.. A gel image showing the protein band is provided with each lot on the Certificate of Analysis. This high-purity specification is suitable for SPR/BLI kinetics measurements, ELISA standard curve development, and antibody epitope binding studies.
Please refer to the Certificate of Analysis (CoA) for reconstitution instructions for your specific lot. Storage: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (A Prepare single-use working aliquots after reconstitution to avoid repeated freeze-thaw cycles. Lyophilized protein is stable for 12 months at −80°C from the date of receipt.
Options listed in “availability content”; other statuses may take longer. Contact BioHippo to confirm current availability and place time-sensitive orders. For large-scale or bulk requirements, extended lead times may apply for custom production runs.
BioHippo offers flexible sourcing for qualified research institutions and partners.
- Alternative tag formats: Different Fc isotypes (hFc vs mFc), His-tag, or biotinylated variants may be available upon request.
- Biotinylated proteins: Site-specific biotinylation for SPR/BLI streptavidin-coated chip immobilisation.
- Nanodisc preparations: Full-length transmembrane protein in synthetic nanodisc format for soluble binding studies.
- Bulk quantities: mg-scale production for high-throughput screening.
- Custom species variants: Mouse, rat, or non-human primate orthologs may be available.
Contact BioHippo customer support for custom requirements.
