| Field | Specification |
|---|---|
| Mfr No | |
| Detection Range | |
| Product Type | |
| Sample Type(s) | cell culture supernatant |
| Species | |
| Storage | |
| Target |
Assay Principle
Tribioscience’s Fast Human Glucagon ELISA is a solid phase ELISA designed to measure glucagon levels in cell culture supernatants. serum, and plasma. The main feature is that the kit uses our novel proprietary approaches to make easy combinations of samples and detection into a one-step instead of the complicated traditional methods. It makes the assay simple, easy, accurate, and ultra-sensitive. The measurement can detect a concentration as low as 10 pg/mL, and finish in 2 hours, with no need for 5-6 hours. The detection range is from 10 to 2000 pg/mL. The levels of human glucagon samples are parallel to the standard curves obtained using the kit standards linearly. Therefore, the kit can be used to determine relative mass values for natural human glucagon.
The kit contains sufficient materials to run an ELISA on one 96-well plate.
Standard Curve Related Products Human IL-1ß ELISA (TBS3219)
Human Glucagon ELISA Kit is a Solid-phase ELISA for the quantitative determination of Glucagon. The microplate is pre-coated with a capture antibody specific to the target. Target present in the sample or standard binds the immobilized antibody, is detected with a biotinylated detection antibody and streptavidin-HRP, and quantified colorimetrically against a standard curve.
Kit Components
The kit provides a pre-coated microplate, target standard for standard-curve generation, detection reagents (detection antibody and enzyme conjugate), wash buffer, substrate, and stop solution. Refer to the product datasheet for the complete component list and quantities.
Performance Data
| Target | Glucagon |
|---|---|
| Species | Human |
| Sample Type | cell culture supernatant |
| Test Principle | Solid-phase ELISA |
| Detection Range | 10–2000 pg/mL |
| Standard | Recombinant Human Glucagon |
| Storage | Store the unopened kit at 2-8°C. | Components: Return unused wells to the foil pouch. Reseal along the entire edge of the zip-seal. May be stored for up to 1 month at 2-8°C.; Aliquot and store at -20°C for up to 1 month in a manual defrost freezer. Avoid repeated freeze-thaw cycles.; May be stored for up to 3 months at 2-8°C. |
✓ Quality tested for reproducibility and lot-to-lot consistency
Safety & Handling
This kit is validated for cell culture supernatant. Other matrices may require additional in-lab validation.
This assay has a detection range of 10–2000 pg/mL. Samples reading outside the range should be diluted or concentrated.
The kit provides a pre-coated microplate, target standard for standard-curve generation, detection reagents (detection antibody and enzyme conjugate), wash buffer, substrate, and stop solution. See the datasheet for the full component list and quantities.
A standard microplate reader capable of measuring absorbance at 450 nm is required for this sandwich ELISA.
Customization & Add-ons: Need this ELISA kit tailored to your workflow? We can help with bulk or custom pack sizes, species or analyte variants, matched antibody pairs and standards, alternate plate formats (48- or 96-well, strip-well), and assay development or custom ELISA solutions for targets not in our catalog. Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services — our team will follow up with feasibility details and next steps.
