| Field | Specification |
|---|---|
| Target | |
| Alternative names | Interleukin-6 receptor subunit beta;IL-6 receptor subunit beta;IL-6R subunit beta;IL-6R-beta;IL-6RB;CDw130;Interleukin-6 signal transducer;Membrane glycoprotein 130;gp130;Oncostatin-M receptor subunit alpha;CD130;IL6ST; |
| Gene ID | |
| UniProt # | |
| Reactivity | |
| Applications | |
| Immunogen | Expression system for standard: NS0; Immunogen sequence: L24-E619 |
| Expression system | |
| Expression region | |
| Assay type | |
| Sample type(s) | Cell Culture Supernatant, Serum, Plasma |
| Sensitivity | |
| Detection range | |
| Assay time | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Assay Principle
This sandwich ELISA quantifies human interleukin-6 receptor subunit beta (gene IL6ST) in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Strips are pre-coated with a capture antibody. After the sample is added, a biotinylated detection antibody binds the captured protein, and an avidin–biotin–peroxidase complex supplies the enzyme. TMB develops color in proportion to the bound target; the reaction is stopped and read at 450 nm, and concentrations are read from the standard curve.
Glycoprotein 130 (gp130, encoded by IL6ST) is the shared signal-transducing receptor subunit for the IL-6 cytokine family, including IL-6, IL-11, LIF, OSM and CNTF. Upon cytokine binding it forms homodimers or heterodimers with LIFR or OSMR and activates JAK kinases and STAT3 signaling, underlying roles in immunity, hematopoiesis and development.
Kit Components
| Anti-Human IL6ST Pre-coated 96-well strip microplate | 1 plate (96 wells) |
|---|---|
| Human IL6ST Standard | 2 vials, 10 ng/tube |
| Human IL6ST Biotinylated antibody (100x) | 100 µL |
| Avidin-Biotin-Peroxidase Complex (100x) | 100 µL |
| Sample Diluent | 30 mL |
| Antibody Diluent | 12 mL |
| Avidin-Biotin-Peroxidase Diluent | 12 mL |
| Color Developing Reagent (TMB) | 10 mL |
| Stop Solution | 10 mL |
| Wash Buffer (25x) | 20 mL |
| Adhesive plate sealers | 4 |
Also required (not supplied): microplate reader for 450 nm; incubator; automated plate washer (optional); precision pipettes for 0.5 µL through 1 mL volumes of aqueous solutions; multichannel pipettes (recommended for many samples); deionized or distilled water; 500 mL graduated cylinders; test tubes for dilution.
Kit components are not sold separately.
Performance Data
| Species | Human |
|---|---|
| Sample types | Cell culture supernatants, serum and plasma (heparin, EDTA, citrate) |
| Assay range | 125 pg/mL–8,000 pg/mL |
| Sensitivity | <10 pg/mL |
| Standard | Recombinant human IL6ST (Leu24–Glu619), expressed in NS0 |
| Cross-reactivity | None detected against related proteins in supplier testing |
| Intra-assay CV | 4.6–7.1% (3 samples, n = 16 each) |
| Inter-assay CV | 6.3–7.5% (3 samples, n = 24 each) |
| Format | 96 wells, removable strips |
Sample dilution: start at 1:200. In the supplier’s tests, serum and plasma samples measured about 190–446 ng/mL.
Example standard curve (OD450, pg/mL): 0 = 0.029; 125 = 0.078; 250 = 0.143; 500 = 0.234; 1000 = 0.424; 2000 = 0.846; 4000 = 1.289; 8000 = 1.909. Run a new standard curve on every plate.
Storage & Handling
Store at 4 °C for up to 6 months or −20 °C for up to 12 months. Avoid repeated freeze–thaw cycles. Ships on gel ice and tolerates up to 3 days at room temperature; freeze on arrival.
Safety
Sample data
| Concentration (pg/ml) | 0 | 125 | 250 | 500 | 1000 | 2000 | 4000 | 8000 |
| O.D. | 0.029 | 0.078 | 0.143 | 0.234 | 0.424 | 0.846 | 1.289 | 1.909 |
Intra/inter assay consistency
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 186 | 1115 | 3384 | 178 | 1076 | 3150 |
| Standard deviation | 13.2 | 73.59 | 156.6%6 | 12.63 | 80.7 | 198.45 |
| CV (%) | 7.1% | 6.6% | 4.6% | 7.1% | 7.5% | 6.3% |
Kit components
Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4Materials required but not provided
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Zhang Di et al. (2022) LC-MS/MS based metabolomics and proteomics reveal candidate biomarkers and molecular mechanism of early IgA nephropathy. Clinical Proteomics. 10.1186/s12014-022-09387-5
Chen Guojie et al. (2024) NUSAP1 Promotes Immunity and Apoptosis by the SHCBP1/JAK2/STAT3 Phosphorylation Pathway to Induce Dendritic Cell Generation in Hepatocellular Carcinoma. Journal of Immunotherapy. 10.1097/CJI.0000000000000531
Narjes Babchia et al. (2019) The bidirectional crosstalk between metastatic uveal melanoma cells and hepatic stellate cells engenders an inflammatory microenvironment. Experimental Eye Research. 10.1016/j.exer.2019.02.012
