| Field | Specification |
|---|---|
| Organism | |
| Applications | |
| Tissue | |
| Disease | FAB M2 Acute Myeloid Leukemia |
| Age | |
| Sex | |
| Growth properties | |
| Biosafety level | |
| Product format | Frozen |
| Storage | |
| Shipping | |
| Catalog no. (Mfr.) | |
| Main SKU |
Overview
The AML14 cell line morphologically appears undifferentiated when grown continuously in suspension culture without the addition of exogenous hematopoietic growth factors. AML14 cells can be induced into eosinophilic differentiation with a combination of IL-3, GM-CSF, and IL-5.
Key elements and design rationale
- Model identity: Human Hematopoietic‐Myeloid Leukemia Cell Line (AML14) is supplied as a tumor cell line derived from Human peripheral blood.
- Growth properties: Suspension, Immature myeloid precursors
- Growth conditions: PriCoat™ T25 Flasks (G299) are recommended for optimal cell culture. RPMI 1640 Medium (TM503) + 8% FBS + 2 mM L-Glutamine (G275) + 1 mM Sodium Pyruvate (TM057) + 50 µM 2-Mercaptoethanol (CH045) + 1% Penicillin/Streptomycin Solution (G255), 37.0°C, 5% CO₂.
- Product format: Frozen, BSL-2
This cell-based model is generally used in immunology, hematology, and signaling studies. Donor/background information is available for contextual interpretation.
Biological background
Therefore, this cell line is suitable for research aiming to understand the regulation and moelcular mechanisms of eosinophil growth and differentiation. Additional cell lines from this panel: Cat. T8172 - Human Hematopoietic‐Myeloid Leukemia Cell Line (AML14.3D10) Donor/background information provided for this product: Male, 68, FAB M2 Acute Myeloid Leukemia.
Research relevance and current trends
- Cell-line models continue to be used for tumor phenotype comparison, pathway perturbation studies, and assay development in controlled in vitro systems.
- Engineered and subtype-defined tumor lines are often used to compare growth behavior, reporter output, and response patterns across matched experimental conditions.
- When metastatic or lineage features are described, investigators commonly interpret results alongside morphology, passage history, and culture environment.
Common research applications
- Cancer biology studies that compare proliferation-associated behavior, morphology, and pathway responses in vitro.
- Assay development for treatment response, reporter monitoring, or phenotype comparison under matched culture conditions.
- Side-by-side comparison of engineered versus parental background characteristics when relevant to the study design.
Changes in morphology, growth rate, viability, or reporter signal are typically interpreted together with passage history, culture matrix, and the specified growth conditions for the model.
Notes for experimental interpretation
- Morphology, doubling behavior, and reporter or marker output can shift with passage number, substrate choice, and medium composition; these variables should be recorded alongside experimental readouts.
- Matched controls such as parental cells, untreated cultures, or parallel cultures maintained under identical conditions help distinguish background effects from biology of interest.
Culture and product details
- Growth Conditions: PriCoat™ T25 Flasks (G299) are recommended for optimal cell culture. RPMI 1640 Medium (TM503) + 8% FBS + 2 mM L-Glutamine (G275) + 1 mM Sodium Pyruvate (TM057) + 50 µM 2-Mercaptoethanol (CH045) + 1% Penicillin/Streptomycin Solution (G255), 37.0°C, 5% CO₂.
- Seeding Density (cells/ml): 30,000 - 35,000 (every 3-4 days) DO NOT ALLOW CELLS TO EXCEED 1x10^6 CELLS/ML
- Thaw cells quickly in a 37°C water bath while agitating gently (maximum 2 minutes). The vial cap should be kept above the water level to minimize the risk of contamination.
- Decontaminate the vial by spraying and wiping the exterior of the vial with 70% ethanol. From this point onwards, all operations should be strictly carried out inside a biological safety cabinet using aseptic conditions.
- Transfer the cell suspension into a 15ml sterile conical tube containing 5ml of pre-warmed, complete growth media. Centrifuge cells at 125xg for 5-7 minutes.
- Aspirate the supernatant without disturbing the cell pellet. Re-suspend the cell pellet in the recommended pre-warmed, complete growth media and dispense into a T25 culture flask.
- Incubate the cells at the recommended conditions.
How should I handle live cells once I receive them?
https://www.abmgood.com/immortalized-cells-documents.html
Following these guidelines will help ensure optimal cell viability and performance.
Why are these cells classified as biosafety level II?
What is your warranty or return policy?
Please refer to the following link for full information:
https://www.abmgood.com/terms
For additional questions, our Order team is happy to assist and can be reached at order@abmgood.com.
How many times can cells divide?
Primary cells have a limited lifespan and will undergo a finite number of population doublings before entering senescence. The exact number varies by cell type and culture conditions.
Immortalized cell lines are capable of extended or indefinite proliferation under proper culture conditions, although growth characteristics may vary between lines.
Do I need Applied Cell Extracellular Matrix (G422) if I am using PriCoat™ flasks?
Cell line sourcing and selection (species, tissue, and disease model matching) · Stable cell line engineering (overexpression, knockdown, knockout via CRISPR/Cas9, shRNA, sgRNA) · Reporter gene integration (GFP, RFP, luciferase, fluorescent/bioluminescent constructs) · Genome editing and knockin (point mutations, tagged endogenous proteins, conditional alleles) · Inducible expression systems (Tet-On/Off and regulatable constructs) · Drug resistance marker selection (puromycin, G418, hygromycin, and others) · Custom growth and media optimisation for specific assay requirements · Scale-up production for high-throughput screening campaigns · Authentication and QC services (STR profiling, mycoplasma testing, viability assessment). Talk to a Scientist or contact support@biohippo.com.
