| Field | Specification |
|---|---|
| Target | |
| Alternative names | Hepatocyte growth factor;Hepatopoietin-A;Scatter factor;SF;Hepatocyte growth factor alpha chain;Hepatocyte growth factor beta chain;HGF;HPTA; |
| Gene ID | |
| UniProt # | |
| Reactivity | |
| Applications | |
| Immunogen | Expression system for standard: NS0; Immunogen sequence: Q32-R494 (alpha) & V495-S728 (beta) |
| Expression system | |
| Expression region | |
| Assay type | |
| Sample type(s) | Cell Culture Supernatant, Serum, Plasma |
| Sensitivity | |
| Detection range | |
| Assay time | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Assay Principle
This sandwich ELISA quantifies human hepatocyte growth factor (gene HGF) in cell culture supernatants, serum and plasma (EDTA, citrate). Strips are pre-coated with a capture antibody. After the sample is added, a biotinylated detection antibody binds the captured protein, and an avidin–biotin–peroxidase complex supplies the enzyme. TMB develops color in proportion to the bound target; the reaction is stopped and read at 450 nm, and concentrations are read from the standard curve.
Hepatocyte growth factor (HGF, also called scatter factor) is a secreted protein that binds and activates the receptor tyrosine kinase MET. Originally identified as a strong mitogen for liver cells, it stimulates growth, motility and morphogenesis in many epithelial and endothelial cell types, and the HGF/MET axis is widely studied in tissue repair, liver regeneration and cancer cell invasion.
Kit Components
| Anti-Human HGF Pre-coated 96-well strip microplate | 1 plate (96 wells) |
|---|---|
| Human HGF Standard | 2 vials, 10 ng/tube |
| Human HGF Biotinylated antibody (100x) | 100 µL |
| Avidin-Biotin-Peroxidase Complex (100x) | 100 µL |
| Sample Diluent | 30 mL |
| Antibody Diluent | 12 mL |
| Avidin-Biotin-Peroxidase Diluent | 12 mL |
| Color Developing Reagent (TMB) | 10 mL |
| Stop Solution | 10 mL |
| Wash Buffer (25x) | 20 mL |
| Adhesive plate sealers | 4 |
Also required (not supplied): microplate reader for 450 nm; incubator; automated plate washer (optional); precision pipettes for 0.5 µL through 1 mL volumes of aqueous solutions; multichannel pipettes (recommended for many samples); deionized or distilled water; 500 mL graduated cylinders; test tubes for dilution.
Kit components are not sold separately.
Performance Data
| Species | Human |
|---|---|
| Sample types | Cell culture supernatants, serum and plasma (EDTA, citrate) |
| Assay range | 125 pg/mL–8,000 pg/mL |
| Sensitivity | <5 pg/mL |
| Standard | Recombinant human HGF (Gln32–Arg494), expressed in NS0 |
| Cross-reactivity | None detected against related proteins in supplier testing |
| Intra-assay CV | 5.8–7.0% (3 samples, n = 16 each) |
| Inter-assay CV | 5.9–8.7% (3 samples, n = 24 each) |
| Format | 96 wells, removable strips |
Sample dilution: start at 1:100. In the supplier’s tests, serum and plasma samples measured 222–1562 pg/mL.
Example standard curve (OD450, pg/mL): 0 = 0.024; 125 = 0.082; 250 = 0.142; 500 = 0.273; 1000 = 0.523; 2000 = 1.03; 4000 = 1.741; 8000 = 2.466. Run a new standard curve on every plate.
Storage & Handling
Store at 4 °C for up to 6 months or −20 °C for up to 12 months. Avoid repeated freeze–thaw cycles. Ships on gel ice and tolerates up to 3 days at room temperature; freeze on arrival.
Safety
Sample data
| Concentration (pg/ml) | 0 | 125 | 250 | 500 | 1000 | 2000 | 4000 | 8000 |
| O.D. | 0.024 | 0.082 | 0.142 | 0.273 | 0.523 | 1.03 | 1.741 | 2.466 |
Intra/inter assay consistency
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 169 | 1246 | 4183 | 168 | 1364 | 4239 |
| Standard deviation | 9.8 | 87.22 | 267.71 | 9.91 | 118.66 | 313.68 |
| CV (%) | 5.8% | 7% | 6.4% | 5.9% | 8.7% | 7.4% |
Kit components
Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4Materials required but not provided
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Can’t Find What You’re Looking For? We can help you source the best match or customize an ELISA solution for your study. Options may include alternative target synonyms, different species reactivity, sample type/matrix compatibility (serum/plasma/lysate/supernatant), assay format (sandwich/competitive), sensitivity/range, detection chemistry (colorimetric/fluorescent/chemiluminescent), plate format (pre-coated/uncoated, strips vs full plate), and bulk or custom packaging. Click Talk to a Scientist to submit a request form, email us at support@biohippo.com, or explore our Research Services for additional support. Our team will be in contact with you shortly.
Baiyao Wang et al. (2022) Cancer-Associated Fibroblasts Promote Radioresistance of Breast Cancer Cells via the HGF/c-Met Signaling Pathway. International Journal of Radiation Oncology Biology Physics. 10.1016/j.ijrobp.2022.12.029
Hao Chang et al. (2013) Surface-mediated functional gene delivery: An effective strategy for enhancing competitiveness of endothelial cells over smooth muscle cells. Biomaterials. 10.1016/j.biomaterials.2013.01.065
Li J et al. (2017) Autophagy-dependent generation of Axin2+ cancer stem-like cells promotes hepatocarcinogenesis in liver cirrhosis. Oncogene. 10.1038/onc.2017.272
Linjie Wang et al. (2023) Biomimetic scaffold-based stem cell transplantation promotes lung regeneration. Bioengineering & Translational Medicine. 10.1002/btm2.10535
Francesca Casciaro et al. (2021) Unravelling Heterogeneity of Amplified Human Amniotic Fluid Stem Cells Sub-Populations. Cells. 10.3390/cells10010158
Tsung-Teng Huang et al. (2022) Blockade of c-Met-Mediated Signaling Pathways by E7050 Suppresses Growth and Promotes Apoptosis in Multidrug-Resistant Human Uterine Sarcoma Cells. International Journal of Molecular Sciences. 10.3390/ijms232314884
Maraldi Tullia et al. (2015) Role of Hepatocyte Growth Factor in the Immunomodulation Potential of Amniotic Fluid Stem Cells. Stem Cells Translational Medicine. 10.5966/sctm.2014-0266
A.A. Soto-Gamez et al. (2023) Mesenchymal stem cell-derived HGF attenuates radiation-induced senescence in salivary glands via compensatory proliferation. Radiotherapy and Oncology. 10.1016/j.radonc.2023.109984
Valentina Marassi et al. (2024) Native characterization and QC profiling of human amniotic mesenchymal stromal cell vesicular fractions for secretome-based therapy. Talanta. 10.1016/j.talanta.2024.126216
Yu Nanlan et al. (2020) Twist1 Contributes to the Maintenance of Some Biological Properties of Dermal Papilla Cells in vitro by Forming a Complex With Tcf4 and β-Catenin. Frontiers in Cell and Developmental Biology. 10.3389/fcell.2020.00824
