| Field | Specification |
|---|---|
| Applications | |
| Assay type | |
| Sample type(s) | Serum | Plasma |
| Sensitivity | |
| Detection range | |
| Detection method | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
The Human HTNV IgG ELISA Kit quantifies human IgG antibodies against HTNV in serum and plasma. The 96-well microplate is pre-coated, and the colorimetric signal is read at 450 nm.
Values are expressed relative to the antibody standard supplied with the kit.
Specifications
| Assay Type | Quantitative |
|---|---|
| Detection Method | Colorimetric (OD 450 nm) |
| Sample Type | Serum, Plasma |
| Sensitivity | 0.25 ng/mL |
| Detection Range | 1.56 - 100 ng/mL |
| Recovery | 80-120% |
| Kit Size | 96 T |
| Shipping | 2-8 °C |
Kit Components
| Component | Quantity |
|---|---|
| ELISA Microtiter plate | 96 wells |
| 20×Concentrated Wash Buffer | 50 mL |
| HRP Conjugate | 12 mL |
| Sample Diluent | 12 mL |
| Substrate Reagent A | 6 mL |
| Substrate Reagent B | 6 mL |
| Stop Solution | 6 mL |
| Positive Control | 1 mL |
| Negative Control | 1 mL |
| Plate Sealer | 3 pieces |
| Sealed Bag | 1 piece |
| Manual | 1 copy |
Assay Procedure
Bring all reagents and samples to room temperature (25 °C) before use. All the reagents should be mixed thoroughly by gently swirling before pipetting. Avoid foaming. The unused ELISA Microtiter plate should be sealed as soon as possible and stored at 2-8 °C.
- Number the sample and control in order (in duplicate), and keep a record of control wells and sample wells. Set 1 well for blank control, 3 wells for negative control and 2 wells for positive control. Samples need test in duplicate (A blank well is not needed with dual-wavelength reading).
- A) Add 100 µL of Positive/Negative Control to Positive/Negative Control wells, keep the blank control well empty. b) Dilute the tested Serum with Sample Diluent at 1:10 into sample well (add 100 µL of Samp- le Diluent and add 10 µL of Serum sample), mix fully.
- Gently tap the plate to mix thoroughly. Cover the ELISA Microtiter plate with sealer. Incubate for 30 min at 37 °C protected from light.
- Remove the plate sealer and aspirate the liquid of each well. Repeat the washing procedure for 5 times with Wash Buffer and immerse for 30-60 sec each time. Invert the plate and pat it against thick clean absorbent paper (If bubbles exist in the wells, clean tips can be used to prick them).
- Add 100 µL of HRP Conjugate to each well except the blank control well.
- Cover the ELISA Microtiter plate with sealer. Incubate for 30 minutes at 37 °C protected from light.
- Repeat step 4.
- Add 50 µL of Substrate Reagent A and 50 µL of Substrate Reagent B to each well. Gently tap the plate to mix thoroughly. Cover with a new plate sealer. Incubate for 15 min at 37 °C protected from light.
- Add 50 µL of Stop Solution to each well, gently tap the plate to mix thoroughly.
- Set the microplate reader wavelength at 450 nm (it is recommended to set the dual wavelength at 450 nm/630 nm) to detect A value of each well. Blank well is not essential when using dual wavelength 450 nm/630 nm for detection. Note: Read the results within 10 min.
Required but not supplied: a microplate reader for 450 nm (dual wavelength 450 nm / 630 nm recommended), precision pipettes and tips, a 37 °C incubator or water bath, deionized or distilled water, and absorbent paper.
Storage & Handling
For Research Use Only. Not for use in diagnostic procedures.
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