| Field | Specification |
|---|---|
| UniProt # | |
| Applications | |
| Assay type | |
| Sample type(s) | Serum | EDTA Plasma | Heparin Plasma | Cell Culture Supernatant |
| Sensitivity | |
| Detection range | |
| Detection method | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
The Human ICAM-1/CD54 ELISA Kit is a sandwich ELISA kit for quantitative measurement of Human intercellular adhesion molecule 1 / ICAM-1 / CD54 in serum, EDTA plasma, heparin plasma and cell culture supernatant. The 96-well microplate is pre-coated, and the colorimetric signal is read at 450 nm.
In this sandwich format, Human intercellular adhesion molecule 1 / ICAM-1 / CD54 is captured in the pre-coated wells and then bound by a detection reagent, so the optical density rises with the Human intercellular adhesion molecule 1 / ICAM-1 / CD54 concentration. Concentrations are calculated from the standard curve run on the same plate.
Specifications
| Target | ICAM1 |
|---|---|
| UniProt | P05362 |
| Assay Type | Sandwich (Quantitative) |
| Detection Method | Colorimetric (OD 450 nm) |
| Sample Type | Serum, EDTA Plasma, Heparin Plasma, Cell Culture Supernatant |
| Sensitivity | 0.13 ng/mL |
| Detection Range | 0.47 - 30 ng/mL |
| Recovery | 80-120% |
| Precision | Intra-assay CV <15%; Inter-assay CV <15% |
| Kit Size | 96 T |
| Shipping | 2-8 °C |
Assay Procedure (Summary)
Bring all reagents to room temperature and prepare working solutions and the standard series before starting.
- Add 100 µL standard or sample; incubate 1 h at 37 °C.
- Wash 3 times with 300 µL wash buffer per well.
- Add 100 µL Detection A working solution; incubate 1 h at 37 °C.
- Wash 3 times with 300 µL wash buffer per well.
- Add 100 µL Detection B working solution; incubate 30 min at 37 °C.
- Wash 5 times with 300 µL wash buffer per well.
- Add 100 µL Color Reagent; incubate 15 min at 37 °C, protected from light.
- Add 50 µL stop solution; the color turns from blue to yellow.
- Read the OD at 450 nm within 10 min (wavelength correction at 630 nm or 620 nm).
Total incubation time is about 2 h 45 min, plus washing steps. Required but not supplied: a microplate reader for 450 nm (correction at 630 nm or 620 nm), pipettes and tips, deionized or distilled water, a 500 mL graduated cylinder, a squirt bottle, manifold dispenser or plate washer, and tubes for standard dilutions.
Sample Preparation
- Serum: use a serum separator tube, let the sample clot for 30 minutes at room temperature, and centrifuge for 15 minutes at 1000 × g.
- Plasma: collect with EDTA or heparin as the anticoagulant and centrifuge for 15 minutes at 1000 × g within 30 minutes of collection.
- Assay samples promptly or store aliquots at -20 °C or below; avoid repeated freeze-thaw cycles.
- Serum and plasma samples: a 10-fold starting dilution in Standard Diluent is suggested; adjust it so readings fall within the standard curve.
- Other sample types (cell culture supernatant): validate dilution and recovery before testing.
Storage & Handling
For Research Use Only. Not for use in diagnostic procedures.
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