| Field | Specification |
|---|---|
| Target | |
| Alternative names | Intercellular adhesion molecule 1;ICAM-1;Major group rhinovirus receptor;CD54;ICAM1; |
| Gene ID | |
| UniProt # | |
| Reactivity | |
| Applications | |
| Immunogen | Expression system for standard: NS0; Immunogen sequence: Q28-E480 |
| Expression system | |
| Expression region | |
| Assay type | |
| Sample type(s) | Cell Culture Supernatant, Cell Lysate, Serum, Plasma |
| Sensitivity | |
| Detection range | |
| Assay time | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Assay Principle
This sandwich ELISA quantifies human intercellular adhesion molecule 1 (gene ICAM1) in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Strips are pre-coated with a capture antibody. After the sample is added, a biotinylated detection antibody binds the captured protein, and an avidin–biotin–peroxidase complex supplies the enzyme. TMB develops color in proportion to the bound target; the reaction is stopped and read at 450 nm, and concentrations are read from the standard curve.
ICAM-1 (CD54) is a cell surface glycoprotein of the immunoglobulin superfamily that is strongly induced on endothelial and immune cells by inflammatory cytokines. It binds the integrins LFA-1 and Mac-1 on leukocytes, supporting their firm adhesion and passage across blood vessel walls as well as T-cell activation, and it is a widely used marker of endothelial activation and inflammation.
Kit Components
| Anti-Human ICAM1 Pre-coated 96-well strip microplate | 1 plate (96 wells) |
|---|---|
| Human ICAM1 Standard | 2 vials, 10 ng/tube |
| Human ICAM1 Biotinylated antibody (100x) | 100 µL |
| Avidin-Biotin-Peroxidase Complex (100x) | 100 µL |
| Sample Diluent | 30 mL |
| Antibody Diluent | 12 mL |
| Avidin-Biotin-Peroxidase Diluent | 12 mL |
| Color Developing Reagent (TMB) | 10 mL |
| Stop Solution | 10 mL |
| Wash Buffer (25x) | 20 mL |
| Adhesive plate sealers | 4 |
Also required (not supplied): microplate reader for 450 nm; incubator; automated plate washer (optional); precision pipettes for 0.5 µL through 1 mL volumes of aqueous solutions; multichannel pipettes (recommended for many samples); deionized or distilled water; 500 mL graduated cylinders; test tubes for dilution.
Kit components are not sold separately.
Performance Data
| Species | Human |
|---|---|
| Sample types | Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) |
| Assay range | 156 pg/mL–10,000 pg/mL |
| Sensitivity | <10 pg/mL |
| Standard | Recombinant human ICAM1 (Gln28–Glu480), expressed in NS0 |
| Cross-reactivity | None detected against related proteins in supplier testing |
| Intra-assay CV | 4.3–5.8% (3 samples, n = 16 each) |
| Inter-assay CV | 5.2–6.5% (3 samples, n = 24 each) |
| Format | 96 wells, removable strips |
Sample dilution: start at 1:100. In the supplier’s tests, serum and plasma samples measured about 100–150 ng/mL.
Example standard curve (OD450, pg/mL): 0 = 0.056; 156 = 0.097; 312 = 0.151; 625 = 0.242; 1250 = 0.434; 2500 = 0.843; 5000 = 1.489; 10000 = 2.184. Run a new standard curve on every plate.
Storage & Handling
Store at 4 °C for up to 6 months or −20 °C for up to 12 months. Avoid repeated freeze–thaw cycles. Ships on gel ice and tolerates up to 3 days at room temperature; freeze on arrival.
Safety
Sample data
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.056 | 0.097 | 0.151 | 0.242 | 0.434 | 0.843 | 1.489 | 2.184 |
Intra/inter assay consistency
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 272 | 1923 | 4746 | 248 | 1929 | 4549 |
| Standard deviation | 14.41 | 111.53 | 204.07 | 14.13 | 125.38 | 236.54 |
| CV (%) | 5.3% | 5.8% | 4.3% | 5.7% | 6.5% | 5.2% |
Kit components
Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4Materials required but not provided
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Jiawen Zou et al. (2025) Coaxial 3D Printing of Blood Vessels for Monitoring Monocyte-Endothelium Interactions and Drug Discovery. Advanced Healthcare Materials. 10.1002/adhm.202404132
Aya A. Eltaibany et al. (2026) Modeling Neuroimmunological Interactions at the Blood–Brain Barrier Using In Vitro 3D Human Organoids: Inflammation and Ischemia–Reperfusion Injury. Cells. 10.3390/cells15131173
Su Yingxue et al. (2019) MicroRNA-181a-5p and microRNA-181a-3p cooperatively restrict vascular inflammation and atherosclerosis. Cell Death & Disease. 10.1038/s41419-019-1599-9
Mingwei Xu et al. (2022) Genetic Arg-304-His substitution in GRK5 protects against sepsis progression by alleviating NF-κB-mediated inflammation. International Immunopharmacology. 10.1016/j.intimp.2022.109629
Bartosz Słomiński et al. (2017) The KL-VS polymorphism of KLOTHO gene is protective against retinopathy incidence in patients with type 1 diabetes. Biochimica et Biophysica ACTA-Molecular Basis of Disease. 10.1016/j.bbadis.2017.12.015
Tong Ming et al. (2020) Elevated Expression of Serum Endothelial Cell Adhesion Molecules in COVID-19 Patients. Journal of Infectious Diseases. 10.1093/infdis/jiaa349
Rui Zhou et al. (2019) miR-499 released during myocardial infarction causes endothelial injury by targeting α7-nAchR. Journal of Cellular and Molecular Medicine. 10.1111/jcmm.14474
Qing-Yu Lu et al. (2024) Luteolin Alleviates the TNF-α-Induced Inflammatory Response of Human Microvascular Endothelial Cells via the Akt/MAPK/NF-κB Pathway. Mediators of Inflammation. 10.1155/mi/6393872
Amanda F Barros et al. (2015) Is there interaction between gut microbial profile and cardiovascular risk in chronic kidney disease patients?. Future Microbiology. 10.2217/fmb.14.140
Paolo Maggi et al. (2017) Cardiovascular risk in advanced naïve HIV-infected patients starting antiretroviral therapy: Comparison of three different regimens - PREVALEAT II cohort. Atherosclerosis. 10.1016/j.atherosclerosis.2017.05.004
