| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Interferon gamma;IFN-gamma;Immune interferon;IFNG; |
| Assay Time | |
| Assay Type | |
| Detection Range | |
| Expression System | |
| Gene ID | |
| Immunogen | Expression system for standard: E.coli; Immunogen sequence: Q24-Q166 |
| Product Type | |
| Reactivity | |
| Sample Type(s) | cell culture supernatants and serum. |
| Sensitivity | |
| Storage | |
| Target | |
| UniProt # |
Background
Also known as: Interferon gamma, IFN-gamma, Immune interferon, IFNG.
Human IFN Gamma/IFNG/Interferon Gamma (IFNG) is a commonly measured biological analyte that can provide insight into cellular state and tissue physiology. This target is frequently investigated in Immunology & Inflammation research contexts. Cytokines and chemokines act as soluble messengers that coordinate immune cell activation, trafficking, and effector functions. Their concentrations can change rapidly in response to infection, tissue injury, or immune stimulation.
Biological function and signaling context
In immune signaling networks, cytokine production is often induced by pattern-recognition pathways and inflammatory transcriptional programs, while feedback regulators can dampen responses to restore homeostasis. Chemokine gradients guide leukocyte migration, influencing which cell populations accumulate at a site and how long they persist.
Why it matters in research
- Immune activation readout: Shifts in abundance can reflect pathway engagement and cellular activation state.
- Microenvironment profiling: Levels can help characterize inflammatory tone in tissues or biofluids.
- Response monitoring: Time-course measurements support interpretation of stimulus, treatment, or infection models.
Disease and translational relevance
Many cytokines and chemokines are reported to associate with inflammatory, autoimmune, infectious, and oncology-related processes. In research settings, interpreting changes benefits from pairing this analyte with complementary markers (e.g., upstream triggers, downstream effectors, and cell-type indicators) and considering matrix effects.
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Sample data
| Concentration (pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
| O.D. | 0.025 | 0.083 | 0.144 | 0.258 | 0.467 | 0.882 | 1.554 | 2.052 |
Intra/inter assay consistency
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 34 | 150 | 469 | 33 | 139 | 437 |
| Standard deviation | 1.73 | 8.7 | 29.54 | 1.91 | 9.73 | 31.9 |
| CV (%) | 7.3% | 5.8% | 7% | 5.8% | 7% | 7.3% |
Kit components
Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4Materials required but not provided
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
►How many samples can I run per plate?
Each PicoKine® kit (96-well format) typically accommodates a 7-point standard curve in duplicate, 2 non-specific binding wells, and up to 39 unknown samples in duplicate. Exact capacity may vary by kit — refer to the datasheet.
►What sample dilution should I use?
Boster recommends performing a pilot study first: run serial dilutions of your samples (e.g. 1:2, 1:4, 1:8, 1:16) to identify the range that falls within the standard curve. This is important because sample matrix and protein expression levels vary significantly by experiment.
►Why is my signal weak or absent?
The most common causes are: (1) target protein below the kit's detection limit — try concentrating samples or reducing dilution; (2) reagents not at room temperature before use; (3) insufficient incubation time; (4) expired or contaminated reagents. See Boster's full ELISA troubleshooting guide at bosterbio.com/protocol-and-troubleshooting/picokine-elisa-troubleshooting.
►Why is my background signal high?
High background is typically caused by insufficient washing (ensure thorough plate draining after each wash step), excess antibody concentration, or contaminated TMB substrate. Increase the number of wash cycles or reduce antibody concentration. Always use fresh substrate solution.
►Are the kit components sterile?
Components are bottled using aseptic techniques and heat-treated vials, but are not guaranteed sterile. If your experiment requires sterile material, filter through a 0.2 µm membrane designed for biological fluids before use.
►How do I analyze my ELISA results?
Plot absorbance (OD 450 nm) against standard concentrations and fit a 4-parameter logistic (4PL) or sigmoidal curve. Read unknown sample concentrations from the curve. Boster provides a free online ELISA data analysis tool at bosterbio.com/biology-research-tools/elisa-data-analysis-online.
►How should I store samples before running the assay?
Aliquot samples before freezing to avoid repeated freeze-thaw cycles, which can degrade the target protein. Store aliquots at -80°C for long-term use. Serum and plasma should be collected, processed, and stored under consistent conditions to minimise pre-analytical variability.
►What positive and negative controls should I include?
Include a positive control (a sample known to contain the target protein at a measurable level) and a negative control (sample matrix without the target, or a sample from a species the kit does not cross-react with). Running controls in every assay validates the assay performance and flags plate-to-plate variability.
Can’t Find What You’re Looking For? We can help you source the best match or customize an ELISA solution for your study. Options may include alternative target synonyms, different species reactivity, sample type/matrix compatibility (serum/plasma/lysate/supernatant), assay format (sandwich/competitive), sensitivity/range, detection chemistry (colorimetric/fluorescent/chemiluminescent), plate format (pre-coated/uncoated, strips vs full plate), and bulk or custom packaging. Click Talk to a Scientist to submit a request form, email us at support@biohippo.com, or explore our Research Services for additional support. Our team will be in contact with you shortly.
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