| Field | Specification |
|---|---|
| Target | |
| Alternative names | IFNA2; IFNA2A; IFNA2B; IFNA2C; Interferon alpha-2; IFN-alpha-2; Interferon alpha-A; LeIF A |
| Gene ID | |
| UniProt # | |
| Reactivity | |
| Applications | |
| Immunogen | Expression system for standard: E.coli; Immunogen sequence: C24-E189 |
| Expression system | |
| Expression region | |
| Assay type | |
| Sample type(s) | Cell Culture Supernatant, Serum |
| Sensitivity | |
| Detection range | |
| Assay time | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Assay Principle
This sandwich ELISA quantifies human interferon alpha-2 (gene IFNA2) in cell culture supernatants and serum. Strips are pre-coated with a capture antibody. After the sample is added, a biotinylated detection antibody binds the captured protein, and an avidin–biotin–peroxidase complex supplies the enzyme. TMB develops color in proportion to the bound target; the reaction is stopped and read at 450 nm, and concentrations are read from the standard curve.
Interferon alpha-2 (IFNA2) is a type I interferon produced by leukocytes, including plasmacytoid dendritic cells and macrophages, in response to viral infection. It signals through the IFNAR1/IFNAR2 receptor and the JAK-STAT pathway to induce antiviral, antiproliferative and immunomodulatory genes, and it is one of the most studied interferon subtypes.
Kit Components
| Anti-Human IFNA2 Pre-coated 96-well strip microplate | 1 plate (96 wells) |
|---|---|
| Human IFNA2 Standard | 2 vials, 1 ng/tube |
| Human IFNA2 Biotinylated antibody (100x) | 100 µL |
| Avidin-Biotin-Peroxidase Complex (100x) | 100 µL |
| Sample Diluent | 30 mL |
| Antibody Diluent | 12 mL |
| Avidin-Biotin-Peroxidase Diluent | 12 mL |
| Color Developing Reagent (TMB) | 10 mL |
| Stop Solution | 10 mL |
| Wash Buffer (25x) | 20 mL |
| Adhesive plate sealers | 4 |
Also required (not supplied): microplate reader for 450 nm; incubator; automated plate washer (optional); precision pipettes for 0.5 µL through 1 mL volumes of aqueous solutions; multichannel pipettes (recommended for many samples); deionized or distilled water; 500 mL graduated cylinders; test tubes for dilution.
Kit components are not sold separately.
Performance Data
| Species | Human |
|---|---|
| Sample types | Cell culture supernatants and serum |
| Assay range | 3.12 pg/mL–200 pg/mL |
| Sensitivity | <1 pg/mL |
| Standard | Recombinant human IFNA2 (Cys24–Glu189), expressed in E. coli |
| Cross-reactivity | None detected against related proteins in supplier testing |
| Intra-assay CV | 3.9–4.3% (3 samples, n = 16 each) |
| Inter-assay CV | 5.1–5.9% (3 samples, n = 24 each) |
| Format | 96 wells, removable strips |
Sample dilution: start at 1:1. In the supplier’s tests, serum samples measured below the lowest standard.
Example standard curve (OD450, pg/mL): 0 = 0.014; 3.12 = 0.039; 6.25 = 0.076; 12.5 = 0.12; 25 = 0.255; 50 = 0.485; 100 = 1.02; 200 = 1.955. Run a new standard curve on every plate.
Storage & Handling
Store at 4 °C for up to 6 months or −20 °C for up to 12 months. Avoid repeated freeze–thaw cycles. Ships on gel ice and tolerates up to 3 days at room temperature; freeze on arrival.
Safety
Sample data
| Concentration (pg/ml) | 0 | 3.12 | 6.25 | 12.5 | 25 | 50 | 100 | 200 |
| O.D. | 0.014 | 0.039 | 0.076 | 0.12 | 0.255 | 0.485 | 1.02 | 1.955 |
Intra/inter assay consistency
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 5 | 19 | 69 | 4 | 21 | 72 |
| Standard deviation | 0.21 | 0.82 | 2.69 | 0.20 | 1.24 | 4.10 |
| CV(%) | 4.2% | 4.3% | 3.9% | 5.1% | 5.9% | 5.7% |
Kit components
Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4Materials required but not provided
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Lu Wang et al. (2024) Decreased plasma docosahexaenoic acid concentration in chronic obstructive pulmonary disease patients with pulmonary Hypertension: Findings from human lipidomics and transcriptomics analysis. Clinica Chimica ACTA. 10.1016/j.cca.2024.119899
Zhang Yu et al. (2011) A novel atherogenic epitope from Mycobacterium tuberculosis heat shock protein 65 enhances atherosclerosis in rabbit and LDL receptor-deficient mice. Heart and Vessels. 10.1007/s00380-011-0183-8
