| Field | Specification |
|---|---|
| Analyte | |
| Analyte category | |
| Organism | |
| Applications | |
| Assay type | |
| Assay target | |
| Assay area | |
| Target type | |
| Sample type(s) | Serum, Plasma, Cell Culture Supernatant |
| Detection method | |
| Detection instrument(s) | Flow Cytometer (Luminex-compatible or equivalent) |
| Instrument required | |
| Assay time | |
| Storage | |
| Shipping | |
| Catalog no. (Mfr.) | |
| Main SKU |
Overview
Flow Cytometry Assay pre-mixed antigen standard. For use with Super-X PlexTM Human IGF-I/ IGF-II Panel assays:
Panel
Pre-Mixed Antigen Standards
Note
Order this item if additional standard is required for on-going assays. This item is included with single or multiplex panels of above analytes.
Kit Contents
- Cytokine Standard
- Concentration (pg/mL) Human IGF-I 20,000 pg/mL Human IGF-II 20,000
- USE: Reconstitute with 250 uL of the standard diluent
- ( Provided with Sample Diluent Kit ) to provide the
- above listed concentrations.
- Preparation of IGF-I/ IGF-II standard curve:
- Prepare a 4X Serial Dilution using the following table:
- ( Prepare enough for standard in duplicate)
- 150 uL total per well. For each new dilution, Mix by
- pipetting up/down 5 times. Change Pipette tips after
- each dilution. Keep standards on ice until use. Standard Conc. (pg/mL) 20,000 pg/mL (Undiluted)
- 1:4 5,000 pg/mL
- 1:16 1,250 pg/mL
- 1:64 313 pg/mL
- 1:256 78 pg/mL
- 1:1024 20 pg/mL
- 1:4096 5 pg/mL Blank 0 pg/mL
- Volume from Previous +
- Standard Diluent ------
- Reconstituted Above
- 50 uL
- 50 uL
- 50 uL
- 50 uL
- 50 uL
- 50 uL _---- + + + + + + + 150 ul 150 ul 150 ul 150 ul 150 ul 150 ul 150 ul
- NOTE: Order this item if additional standard is required
- for on-going assays. This item is included with single
- or multiplex panels of above analytes.
Storage
Store Refrigerated ( 4-8°C.) prior to
⚠ Caution
Dispose contents carefully, flush with water to avoid reaction with lead drain lines.
| Parameter | Value |
|---|---|
| Sample Volume | 15.0 µL per test |
| Intra-Assay CV | <10% |
| Inter-Assay CV | <20% |
| Recovery | 70–130% |
| Cross-Reactivity (within panel) | Negligible |
| Approximate Assay Time | ~3 Hours |
Performance data sourced from Antigenix America product datasheet. Actual values may vary by lot. Refer to certificate of analysis supplied with kit.
What sample types are compatible?
This Human Super-X Plex™ assay is compatible with serum, plasma, cell culture supernatant, and cell lysate samples. A species-matched Sample Diluent Kit (DKH100 (Human/Primate Serum/Plasma)) must be ordered separately.
What instrument is required?
A flow cytometer capable of detecting PE fluorescence is required. The assay is compatible with Luminex-based platforms and standard flow cytometers with bead-based multiplex detection capability.
What analytes are included in this panel?
IGF-1, IGF-II.
How much sample is needed per test?
15 µL per test. 2 Plex kit provides sufficient reagents for a full standard curve plus samples.
Looking for a specific analyte, species, or panel configuration not listed? The Super-X Plex™ platform is designed for flexibility — from off-the-shelf single-plex assays to fully configured custom multi-plex panels and bespoke kit builds.
Custom Multi-Plex Panel Design
Individual Super-X Plex™ assays within the same species group can be combined into custom multiplex configurations without additional cross-reactivity validation. Select any combination of analytes from the available group catalog — from 2-plex to 24-plex — and receive a single pre-mixed standard and matched bead set. Custom panels are available for Human, Mouse, Rat, Canine, and Non-Human Primate matrices.
Custom Kit Services
Beyond panel configuration, BioHippo and Antigenix America offer extended custom kit services to support specialized study designs:
- Analyte selection: Access to the full Super-X Plex™ catalog, including analytes not listed online. If your target is not shown, contact us — additional cytokines, chemokines, growth factors, and soluble receptors may be available.
- Species expansion: Custom assay development for species beyond the standard catalog (Equine, Feline, Porcine, Bovine, and others) may be available upon inquiry.
- Sample matrix compatibility: Assays are validated for serum, plasma, cell culture supernatant, and cell lysate. Custom matrix validation or adjusted diluent formulations can be explored for non-standard sample types.
- Kit size and format: Standard kits are supplied as 96-test formats. Smaller pilot formats (32-test) are available for select panels. Bulk quantities and custom packaging for high-throughput or multi-site studies can be quoted on request.
- Pre-mixed standard panels: Custom pre-mixed lyophilized standards can be formulated to match your exact panel composition, simplifying standard curve preparation across multiple assay runs.
- White-label and OEM supply: For core facilities, CROs, or institutional programs requiring custom-labeled or privately packaged kits, OEM supply arrangements can be discussed.
How to Request
Click Talk to a Scientist to submit a customization request form, email us at support@biohippo.com, or explore our Research Services for additional support options. Our team will review your requirements and respond promptly.
