| Field | Specification |
|---|---|
| Target | |
| Alternative names | Insulin-like growth factor-binding protein 7;IBP-7;IGF-binding protein 7;IGFBP-7;IGFBP-rP1;MAC25 protein;PGI2-stimulating factor;Prostacyclin-stimulating factor;Tumor-derived adhesion factor;TAF;IGFBP7;MAC25, PSF; |
| Gene ID | |
| UniProt # | |
| Reactivity | |
| Applications | |
| Immunogen | Expression system for standard: NS0; Immunogen sequence: D30-L282 |
| Expression system | |
| Expression region | |
| Assay type | |
| Sample type(s) | Cell Culture Supernatant, Serum, Plasma, Urine |
| Sensitivity | |
| Detection range | |
| Assay time | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Assay Principle
This sandwich ELISA quantifies human insulin-like growth factor-binding protein 7 (gene IGFBP7) in cell culture supernatants, serum, plasma (heparin, EDTA) and urine. Strips are pre-coated with a capture antibody. After the sample is added, a biotinylated detection antibody binds the captured protein, and an avidin–biotin–peroxidase complex supplies the enzyme. TMB develops color in proportion to the bound target; the reaction is stopped and read at 450 nm, and concentrations are read from the standard curve.
IGFBP-7 (also called IGFBP-rP1 or mac25) is a secreted protein related to the IGF-binding proteins but binding IGF-1 and IGF-2 only weakly. It promotes cell adhesion, stimulates prostacyclin production and interacts with CD93 in angiogenesis, and it is studied in vascular biology, senescence and kidney injury.
Kit Components
| Anti-Human IGFBP7 Pre-coated 96-well strip microplate | 1 plate (96 wells) |
|---|---|
| Human IGFBP7 Standard | 2 vials, 40 ng/tube |
| Human IGFBP7 Biotinylated antibody (100x) | 100 µL |
| Avidin-Biotin-Peroxidase Complex (100x) | 100 µL |
| Sample Diluent | 30 mL |
| Antibody Diluent | 12 mL |
| Avidin-Biotin-Peroxidase Diluent | 12 mL |
| Color Developing Reagent (TMB) | 10 mL |
| Stop Solution | 10 mL |
| Wash Buffer (25x) | 20 mL |
| Adhesive plate sealers | 4 |
Also required (not supplied): microplate reader for 450 nm; incubator; automated plate washer (optional); precision pipettes for 0.5 µL through 1 mL volumes of aqueous solutions; multichannel pipettes (recommended for many samples); deionized or distilled water; 500 mL graduated cylinders; test tubes for dilution.
Kit components are not sold separately.
Performance Data
| Species | Human |
|---|---|
| Sample types | Cell culture supernatants, serum, plasma (heparin, EDTA) and urine |
| Assay range | 625 pg/mL–40,000 pg/mL |
| Sensitivity | <20 pg/mL |
| Standard | Recombinant human IGFBP7 (Asp30–Leu282), expressed in NS0 |
| Cross-reactivity | None detected against related proteins in supplier testing |
| Intra-assay CV | 7.0–7.5% (3 samples, n = 16 each) |
| Inter-assay CV | 7.3–8.1% (3 samples, n = 24 each) |
| Format | 96 wells, removable strips |
Sample dilution: start at 1:10. In the supplier’s tests, serum and plasma samples measured about 50 ng/mL.
Example standard curve (OD450, ng/mL): 0 = 0.01; 0.625 = 0.072; 1.25 = 0.139; 2.5 = 0.253; 5 = 0.481; 10 = 0.842; 20 = 1.437; 40 = 2.327. Run a new standard curve on every plate.
Storage & Handling
Store at 4 °C for up to 6 months or −20 °C for up to 12 months. Avoid repeated freeze–thaw cycles. Ships on gel ice and tolerates up to 3 days at room temperature; freeze on arrival.
Safety
Sample data
| Concentration (ng/ml) | 0 | 0.625 | 1.25 | 2.5 | 5 | 10 | 20 | 40 |
| O.D. | 0.01 | 0.072 | 0.139 | 0.253 | 0.481 | 0.842 | 1.437 | 2.327 |
Intra/inter assay consistency
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 1629 | 4265 | 18882 | 1617 | 4427 | 20605 |
| Standard deviation | 114.03 | 319.87 | 1340.62 | 118.04 | 358.58 | 1669 |
| CV (%) | 7% | 7.5% | 7.1% | 7.3% | 8.1% | 8.1% |
Kit components
Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4Materials required but not provided
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Can’t Find What You’re Looking For? We can help you source the best match or customize an ELISA solution for your study. Options may include alternative target synonyms, different species reactivity, sample type/matrix compatibility (serum/plasma/lysate/supernatant), assay format (sandwich/competitive), sensitivity/range, detection chemistry (colorimetric/fluorescent/chemiluminescent), plate format (pre-coated/uncoated, strips vs full plate), and bulk or custom packaging. Click Talk to a Scientist to submit a request form, email us at support@biohippo.com, or explore our Research Services for additional support. Our team will be in contact with you shortly.
Gabriela Krejčová et al. (2023) Macrophage-derived insulin antagonist ImpL2 induces lipoprotein mobilization upon bacterial infection. EMBO Journal. 10.15252/embj.2023114086
Alfredo G. Casanova et al. (2024) Oxidative stress and renal status of farmers exposed to pesticides in Seville (Spain). Science of the Total Environment. 10.1016/j.scitotenv.2024.175180
Bank Jonna R. et al. (2019) Urinary TIMP-2 Predicts the Presence and Duration of Delayed Graft Function in Donation After Circulatory Death Kidney Transplant Recipients. Transplantation. 10.1097/TP.0000000000002472
Obata Yumi et al. (2023) Clinical usefulness of urinary biomarkers for early prediction of acute kidney injury in patients undergoing transaortic valve implantation. Scientific Reports. 10.1038/s41598-023-46015-0
Kevin Esmeijer et al. (2021) The predictive value of TIMP-2 and IGFBP7 for kidney failure and 30-day mortality after elective cardiac surgery. Scientific Reports. 10.1038/s41598-020-80196-2
Maria Serena Benassi et al. (2015) Tissue and serum IGFBP7 protein as biomarker in high-grade soft tissue sarcoma. American Journal of Cancer Research. PMID: 26807324
de Rooij Esther N. M. et al. (2024) Urinary beta-2 microglobulin increases whereas TIMP-2 and IGFBP7 decline after unilateral nephrectomy in healthy kidney donors. Scientific Reports. 10.1038/s41598-024-62246-1
Chong Chen et al. (2020) Clinical study of serum IGFBP7 in predicting lymphatic metastasis in patients with lung adenocarcinoma. Current Problems in Cancer. 10.1016/j.currproblcancer.2020.100584
Supakit Khacha-ananda et al. (2024) Exploring the link: DNA methylation and kidney injury markers in farmers exposed to glyphosate-surfactant herbicides. Regulatory Toxicology and Pharmacology. 10.1016/j.yrtph.2024.105765
Hui Wun Fung et al. (2025) The use of urinary biomarkers as prognostic tools: predicting kidney outcomes in pediatric acute kidney injury. Pediatric Nephrology. 10.1007/s00467-025-06920-0
