Human IgG1-kappa-[scFv-heavy-kappa]2 Recombinant Isotype Control Antibody (SAA2439)

SKU:BHA21421241
Overview
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Human IgG1-kappa-[scFv-heavy-kappa]2 monoclonal isotype control antibody (clone SAA2439), unconjugated. Serves as a negative control in Flow Cytometry experiments, helping distinguish specific staining from background signal.
Isotype IgG1-kappa-[scFv-heavy-kappa]2
Clone number SAA2439
Clonality Monoclonal
Host Human
Conjugate(s) Unconjugated
Application(s) Flow Cytometry
Options selector
Catalog no. Size
HV080313-20UG 20 ug
HV080313-50UG 50 ug
HV080313-100UG 100 ug
HV080313-1MG 1 mg
Available Options

Select the variant that best fits your experiment. Availability and lead time may vary by option.

  • Options: Size: 20 ug, 50 ug, 100 ug, 1 mg
  • Lead time: options listed in "Availability Content"; other statuses may take longer.
  • Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
  • Shipping: Please contact us to confirm shipping conditions for this product.
  • Upon receipt: store at recommended temperature as soon as possible.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Host Human
Clone SAA2439
Clonality
  • Monoclonal
Isotype
  • IgG1-kappa-[scFv-heavy-kappa]2
Applications
  • Flow Cytometry
Purity >95% as determined by SDS-PAGE.
Purification Protein A/G purified from cell culture supernatant.
Form Liquid
Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Storage buffer 0.01M PBS, pH 7.4.
Catalog no. (Mfr.) HV080313
Main SKU BHA21421241

Product Overview

Isotype Control

Human IgG1-kappa-[scFv-heavy-kappa]2 Recombinant Isotype Control Antibody (SAA2439) is a monoclonal isotype control antibody (clone SAA2439). Isotype control antibodies match the host species, isotype and conjugate of a test antibody but are directed against an antigen absent from the sample, providing a measure of non-specific background binding (Fc-receptor interactions, non-specific membrane adhesion) in immunoassays.

Use this control alongside human-derived IgG1-kappa-[scFv-heavy-kappa]2 primary antibodies to set gating and background thresholds.

Specifications

Host Species Human
Isotype IgG1-kappa-[scFv-heavy-kappa]2
Clonality Monoclonal
Clone Number SAA2439
Conjugate Unconjugated
Form Liquid
Purity >95% as determined by SDS-PAGE.
Purification Protein A/G purified from cell culture supernatant.
Storage Buffer 0.01M PBS, pH 7.4.
Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Applications & Recommended Use

Vendor-listed applications: Flow Cytometry.

Run the isotype control at the same concentration and staining conditions as the corresponding test antibody to obtain a matched background reference.

Storage & Handling

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Supplied in 0.01M PBS, pH 7.4.

Safety

For research use only. Not intended for diagnostic or therapeutic procedures. Follow standard laboratory safety practices when handling.

Q.What is this isotype control used for?
A.It provides a matched negative control: an antibody of the same host, isotype as your test antibody but with no specificity for antigens in your sample, so any signal it produces represents non-specific background.
Q.Which applications is it listed for?
A.The manufacturer lists Flow Cytometry. Use it under the same conditions and concentration as the test antibody in that application.
Q.How do I choose the right isotype control?
A.Match three properties of your test antibody: host species (Human), isotype (IgG1-kappa-[scFv-heavy-kappa]2), and label (unconjugated here). All three must match for the background estimate to be valid.
Q.How should it be stored?
A.Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.

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Experience the power of Celltrypse™, c-LEcta's innovative enzyme solution for gentle and efficient cell dissociation. Request your free sample and discover a superior alternative for your cell culture workflows.

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