| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | huIgG1k, human IgG1 kappa |
| Conjugate | |
| Formulation | |
| Host | |
| Product Type | |
| Shipping | |
| Storage |
Product Overview
Human IgG1k from human myeloma plasma is used as a negative control(1,2) for work involving human IgG1 isotype antibodies or recombinant proteins. The molecular weight of this myeloma derived protein has varied for some lots; running at about 135 kD in SDS-PAGE, compared to the expected 150 kD for normal human IgG1. This was due to a smaller heavy chain, which ran at approximately 40 kD when reduced.
Validated Applications
| Application | Recommended Dilution |
|---|---|
| Flow Cytometry (FC) | See datasheet |
| Immunohistochemistry (IHC) | See datasheet |
| EIA | See datasheet |
| Western Blot (WB) | See datasheet |
Product Specifications
| Clone | Myeloma |
|---|---|
| Clonality | Monoclonal |
| Host Species | Human |
| Isotype | IgG1 kappaappa |
| Species Reactivity | multiple species |
| Conjugate | APC, Biotin, FITC, PE, Unconjugated |
| Storage | Store at 2 - 5°C. Freeze/Thawing is not recommended. |
Immunogen / Antigen
Please refer to product datasheet for immunogen details.
Safety & Handling
For Research Use Only (RUO). Not for diagnostic or therapeutic use. Handle as a potential biohazard in accordance with good laboratory practices.
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This antibody has been validated for FC, IHC, EIA, WB. Always refer to the product datasheet for recommended dilution ranges and appropriate positive control samples for each application type.
Validated reactivity has been confirmed for multiple species. Cross-reactivity with additional species may exist based on sequence homology but has not been confirmed by the manufacturer. For unlisted species, we recommend running a preliminary validation experiment.
This antibody was raised in Human. For indirect detection (WB, IHC, IF), select an anti-Human secondary antibody that matches the isotype. For direct detection, check the variant selector for conjugated formats. Confirm secondary antibody compatibility before ordering.
Please refer to the product datasheet for detailed immunogen information. This data is available on the manufacturer datasheet linked in the Documents tab.
Please refer to the storage conditions on the product label and datasheet. As a general best practice: upon receipt, aliquot into single-use volumes to minimize freeze-thaw cycling, which can progressively reduce antibody performance and lead to aggregation.
Validated reactivity covers multiple species samples. Performance can vary across different cell lines and tissue types depending on target antigen expression levels and epitope accessibility. We recommend verifying target expression in your specific model system using a known positive control before proceeding to experimental samples.
BioHippo offers customization and value-added services for qualified research programs:
- Bulk Pricing: Volume discounts available for orders of 5 or more units. Request a quote through the Contact Us page.
- Custom Conjugation: Select antibody products are available for custom conjugation to fluorophores, biotin, HRP, or other detection labels upon request. Minimum quantities apply.
- Custom Formats: Lyophilized or concentrated formats may be available for select products. Inquire before ordering.
- Technical Support: Our scientific team is available to assist with application optimization, protocol troubleshooting, and experimental design.
For customization inquiries, please use the Contact Us page or reach out to our support team directly.
