| Field | Specification |
|---|---|
| Target | |
| Alternative names | Interleukin-1 alpha;IL-1 alpha;Hematopoietin-1;IL1A;IL1F1; |
| Gene ID | |
| UniProt # | |
| Reactivity | |
| Applications | |
| Immunogen | Expression system for standard: E.coli; Immunogen sequence: S113-A271 |
| Expression system | |
| Expression region | |
| Assay type | |
| Sample type(s) | Cell Culture Supernatant, Serum, Plasma |
| Sensitivity | |
| Detection range | |
| Assay time | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Assay Principle
This single-incubation sandwich ELISA quantifies human interleukin-1 alpha (gene IL1A) in cell culture supernatants, serum and plasma ( heparin, EDTA, citrate). The strips are coated with an anti-tag antibody. Samples and standards are added to the wells together with the kit’s antibody cocktail (Antibody A and Antibody B); the resulting antibody–target complex is held on the plate through the tag. After washing, TMB substrate is added and the HRP label on the detection antibody converts it to a colored product in proportion to the bound target. The reaction is stopped and the plate is read at 450 nm. The supplier markets the Quick format as a 90-minute assay.
Interleukin-1 alpha (IL-1α) is an IL-1 family cytokine stored inside many non-immune cells and released when cells are damaged, acting as an alarmin. It signals through IL1R1 and IL1RAP via MyD88 and IRAK kinases to activate NF-κB and MAPK pathways, and it is studied in sterile inflammation, fever and tissue injury.
Kit Components
| Anti-tag Pre-coated 96-well Strip Microplate | 12 strips of 8 wells |
|---|---|
| Human IL1A Standard | 2 × 10 ng/tube |
| Human IL1A Antibody A | 3 mL |
| Human IL1A Antibody B | 3 mL |
| Sample Diluent | 15 mL |
| TBS-T Wash Buffer (25x) | 12 mL |
| Color Developing Reagent (TMB) | 10 mL |
| Stop Solution | 10 mL |
| Adhesive Plate Sealers | 2 |
Also required (not supplied): microplate reader for 450 nm; automated plate washer (optional); precision pipettes for 0.5 µL through 1 mL volumes of aqueous solutions; multichannel pipettes (recommended for many samples); deionized or distilled water; 500 mL graduated cylinders; test tubes for dilution; horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3 mm.
Performance Data
| Species | Human |
|---|---|
| Sample types | Cell culture supernatants, serum and plasma ( heparin, EDTA, citrate) |
| Assay range | 4.7 pg/mL–300 pg/mL |
| Sensitivity | <0.5 pg/mL |
| Standard | Recombinant human IL1A (Ser113–Ala271), expressed in E. coli |
| Cross-reactivity | None detected against related proteins in supplier testing |
Storage & Handling
Store at 4 °C for up to 6 months. Ships on gel ice and tolerates up to 3 days at room temperature; refrigerate on arrival.
Safety
Sample data
| Concentration (pg/ml) | 0 | 4.7 | 9.4 | 18.8 | 37.5 | 75 | 150 | 300 |
| O.D. | 0.041 | 0.097 | 0.162 | 0.367 | 0.582 | 0.866 | 1.335 | 2.033 |
Intra/inter assay consistency
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 14 | 52 | 99 | 15 | 49 | 103 |
| Standard deviation | 0.98 | 2.49 | 4.35 | 1.06 | 2.4 | 6.07 |
| CV (%) | 7% | 4.8% | 4.4% | 7.1% | 4.9% | 5.9% |
Kit components
Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x)|1|12ml Color Developing Reagent (TMB)|1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|PieceMaterials required but not provided
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional)
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
- Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.
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