| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Interleukin-1 beta; IL-1 beta; Catabolin; IL1B; IL1F2 |
| Assay Time | |
| Assay Type | |
| Detection Range | |
| Expression System | |
| Gene ID | |
| Immunogen | Expression system for standard: E.coli; Immunogen sequence: A117-S269 |
| Product Type | |
| Reactivity | |
| Sample Type(s) | cell culture supernatants, serum and plasma (heparin, EDTA, citrate) |
| Sensitivity | |
| Storage | |
| Target | |
| UniProt # |
Background
Also known as: Interleukin-1 beta, IL-1 beta, Catabolin, IL1B, IL1F2.
Human IL-1 beta (IL1B) is widely studied as a molecular readout in experimental models where changes in protein abundance reflect underlying biology. This target is frequently investigated in Molecular & Cellular Biology research contexts. Cytokines and chemokines act as soluble messengers that coordinate immune cell activation, trafficking, and effector functions. Their concentrations can change rapidly in response to infection, tissue injury, or immune stimulation.
Biological function and signaling context
In immune signaling networks, cytokine production is often induced by pattern-recognition pathways and inflammatory transcriptional programs, while feedback regulators can dampen responses to restore homeostasis. Chemokine gradients guide leukocyte migration, influencing which cell populations accumulate at a site and how long they persist.
Why it matters in research
- Immune activation readout: Shifts in abundance can reflect pathway engagement and cellular activation state.
- Microenvironment profiling: Levels can help characterize inflammatory tone in tissues or biofluids.
- Response monitoring: Time-course measurements support interpretation of stimulus, treatment, or infection models.
Disease and translational relevance
Many cytokines and chemokines are reported to associate with inflammatory, autoimmune, infectious, and oncology-related processes. In research settings, interpreting changes benefits from pairing this analyte with complementary markers (e.g., upstream triggers, downstream effectors, and cell-type indicators) and considering matrix effects.
Sample data
| Concentration (pg/ml) | 0 | 3.9 | 7.8 | 15.6 | 31.2 | 62.5 | 125 | 250 |
| O.D. | 0.022 | 0.074 | 0.105 | 0.249 | 0.423 | 0.707 | 1.095 | 1.830 |
Intra/inter assay consistency
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 23 | 56 | 158 | 26 | 62 | 173 |
| Standard deviation | 1 | 3.4 | 8.5 | 1.6 | 3.9 | 12.4 |
| CV (%) | 4.5% | 6.1% | 5.4% | 6.3% | 6.4% | 7.2% |
Kit components
| Catalog number | Description | Quantity | Storage of opened/reconstituted material |
|---|---|---|---|
| EZ0392-CA | Rabbit anti- human IL1B polyclonal antibody (Capture Antibody) | 500 µl, 0.4 mg/mL (recommended dilution 1:100) | Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. |
| EZ0392-DA | Biotinylated goat anti- human IL1B polyclonal antibody (Detection Antibody) | 500 µl, 25 µg/mL (recommended dilution 1:100) | |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 500 µl (recommended dilution 1:100) | |
| EK0392-ST | Lyophilized recombinant human IL1B standard | 1 ng/tube×3 | Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit. |
Materials required but not provided
- Microplate reader in standard size.
- Automated plate washer.
- Incubator.
- Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
- Clean tubes and Eppendorf tubes.
- 96 well microplate (Cat# AR1100)
- Plate Sealers.
- Capture Antibody Diluent: PBS.
- Reagent Diluent: 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered.
- Substrate Solution: Tetramethylbenzidine (Cat# AR1104)
- Stop Solution: 2 N H2SO4 (Cat# AR1105)
- Wash Buffer (PBS and PBS-T). Recipes:
- PBS: 8g NaCl, 0.2g KCl, 1.15g Na2HPO4, 0.2g KH2PO4, adjust the total volume to 1 L with distilled water, pH 7.2-7.4, 0.2 µm filtered.
- PBS-T: 0.1% Tween® 20 in PBS, pH 7.2-7.4.
- *Item 6 – 12 are included in the EZ Set Accessory Kit (EZA001)
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