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Interleukin 12 (IL-12) is an anti-inflammatory cytokine made up of a 40 kDa (p40) subunit and a 35 kDa (p35) subunit, also known as a natural killer cell stimulatory factor (NKSF) or cytotoxic lymphocyte maturation factor (CLMF). The IL-12 and IL-23 (another heterodimeric cytokine) share the p40 subunit. IL-12 inhibits the synthesis of numbers of cytokines, including IFN-gamma, IL-2, IL-3, TNF, and GM-CSF produced by activated macrophages and by helper T-cells.
Assay Principle
TYPICAL DATA This standard curve(R 2 =1.000) is provided for demonstration only. A standard curve should be generated for each set of samples assayed.
SENSITIVITY The minimum detectable dose (MOD) of human IL-12/IL-23 p40 is typically 5 pg/ml.
The Intra-CV is < 10%, the Inter-CV is < 12%.
Human IL-12/IL-23 p40 ELISA Kit is a Solid-phase sandwich ELISA for the quantitative determination of IL-12/IL-23 p40. The microplate is pre-coated with a capture antibody specific to the target. Target present in the sample or standard binds the immobilized antibody, is detected with a biotinylated detection antibody and streptavidin-HRP, and quantified colorimetrically against a standard curve.
Kit Components
The kit provides a pre-coated microplate, target standard for standard-curve generation, detection reagents (detection antibody and enzyme conjugate), wash buffer, substrate, and stop solution. Refer to the product datasheet for the complete component list and quantities.
Performance Data
| Target | IL-12/IL-23 p40 |
|---|---|
| Species | Human |
| Assay Type | Sandwich |
| Test Principle | Solid-phase sandwich ELISA |
| Sensitivity | 5 pg/mL |
| Standard | Recombinant Human IL-12/IL-23 p40 |
| Specificity | This assay recognizes natural and recombinant human IL-12/IL-23 P40. |
| Storage | Store the unopened kit at 2-8°C. |
✓ Quality tested for reproducibility and lot-to-lot consistency
Safety & Handling
This assay has a sensitivity of 5 pg/mL. Samples reading outside the range should be diluted or concentrated.
The kit provides a pre-coated microplate, target standard for standard-curve generation, detection reagents (detection antibody and enzyme conjugate), wash buffer, substrate, and stop solution. See the datasheet for the full component list and quantities.
A standard microplate reader capable of measuring absorbance at 450 nm is required for this sandwich ELISA.
Customization & Add-ons: Need this ELISA kit tailored to your workflow? We can help with bulk or custom pack sizes, species or analyte variants, matched antibody pairs and standards, alternate plate formats (48- or 96-well, strip-well), and assay development or custom ELISA solutions for targets not in our catalog. Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services — our team will follow up with feasibility details and next steps.
