Human IL-1R3/IL-1RAcP/IL1RAP protein (Recombinant) (C-hFc) (STJP001374)

What it means: Mammalian (HEK293) — this protein was produced using a Mammalian (HEK293) recombinant expression system, which determines its post-translational modification profile, folding, and application suitability.

Why it matters:

• Mammalian systems (HEK293) enable human-like glycosylation and disulfide bond formation — important for receptor-binding assays and cell-based activity studies.
• Insect cell systems (Sf9) offer high-yield expression with partial glycosylation; E. coli systems yield non-glycosylated protein at high quantity.

How to interpret here: For applications requiring native-like glycosylation, Mammalian (HEK293)-expressed protein is preferred. For structural studies or antibody production where glycosylation is not critical, E. coli or insect cell proteins are suitable.

What it means: > 90% by SDS-PAGE. — purity was assessed by SDS-PAGE (and HPLC where stated) to confirm the proportion of the preparation that is the correctly-sized target protein.

Why it matters:

• High purity (>95%) minimises background from host cell contaminants in sensitive assays such as SPR/BLI, activity assays, and cell-based studies.
• Consistent lot-to-lot purity is essential for reproducibility in assay development and quantitative experiments.

How to interpret here: > 90% by SDS-PAGE. means this preparation is suitable for most research applications. Lot-specific purity data (gel image or HPLC trace) is available on the Certificate of Analysis. Contact technical support if ultra-high purity (>99%) is required for biophysical measurements.

What it means: Store at −20°C long-term — this is the recommended condition to maintain peptide or protein integrity, stability, and research-grade performance over time.

Why it matters:

• Storage at −20°C minimises hydrolysis, oxidation, and other degradation reactions that accelerate at higher temperatures.
• Adhering to the stated storage condition is required to maintain the validity of Certificate of Analysis QC data.

How to interpret here: Place the product at −20°C immediately upon receipt. For frequently used products, prepare small working aliquots to avoid repeatedly freeze-thawing the bulk stock.

Tip: Do not store thawed product at 4°C for more than 48 hours. Avoid ultra-low (−80°C) storage unless specified, as some formulations are sensitive to this temperature.

What it means: This peptide is supplied in aqueous solution — Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4.. This formulation preserves peptide stability for use in immunodepletion and competition assays.

Why it matters:

• Ready to use in standard antibody pre-incubation protocols without buffer exchange or reconstitution.
• PBS formulation is compatible with most immunoassay buffers; up to 5% DMSO may be added for poorly soluble peptides.

How to interpret here: Use this peptide directly at a 5–10 molar excess over the antibody concentration. No reconstitution is required. Prepare single-use aliquots upon receipt to preserve the stock.

What it means: UniProt accession Q9NPH3-1 is the stable, unique identifier for this protein sequence and annotation record in the Universal Protein Resource (UniProtKB) database.

Why it matters:

• The UniProt entry provides the canonical sequence, known isoforms, post-translational modification sites, disease associations, and curated literature.
• Cross-referencing results to UniProt enables standardised data reporting and database integration (PDB, STRING, OMIM, Ensembl).

How to interpret here: Use UniProt ID Q9NPH3-1 at uniprot.org to retrieve the full sequence, confirm the expressed region covered by this product, and verify which isoform is represented. The accession is also useful for downstream bioinformatic analyses.