| Field | Specification |
|---|---|
| Target | |
| Alternative names | Interleukin-23 subunit alpha; IL-23 subunit alpha; IL-23-A; Interleukin-23 subunit p19; IL-23p19; IL23A; SGRF |
| Gene ID | |
| UniProt # | |
| Reactivity | |
| Applications | |
| Immunogen | Expression system for standard: NS0; Immunogen sequence: (R20-P189)+(I23-S328) |
| Expression system | |
| Expression region | |
| Assay type | |
| Sample type(s) | Cell Culture Supernatant, Serum, Plasma |
| Sensitivity | |
| Detection range | |
| Assay time | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Assay Principle
This sandwich ELISA quantifies human interleukin-23 subunit alpha (gene IL23A) in cell culture supernatants, serum and plasma (heparin, EDTA). Strips are pre-coated with a capture antibody. After the sample is added, a biotinylated detection antibody binds the captured protein, and an avidin–biotin–peroxidase complex supplies the enzyme. TMB develops color in proportion to the bound target; the reaction is stopped and read at 450 nm, and concentrations are read from the standard curve.
IL-23 p19 (IL23A) is the subunit that combines with p40 (IL12B) to form the cytokine IL-23, released by dendritic cells and macrophages. IL-23 signals through IL-12Rβ1 and IL-23R to activate JAK2, TYK2 and STAT3, supporting the expansion and survival of IL-17–producing Th17 cells. It is a central target in studies of chronic inflammation and autoimmunity.
Kit Components
| Anti-Human IL23A Pre-coated 96-well Strip Microplate | 12 strips of 8 wells |
|---|---|
| Human IL23A Standard | 2 × 10 ng/tube |
| Human IL23A Biotinylated Antibody (100x) | 100 µL |
| Avidin-Biotin-Peroxidase Complex (100x) | 100 µL |
| Sample Diluent | 30 mL |
| Antibody Diluent | 12 mL |
| Avidin-Biotin-Peroxidase Diluent | 12 mL |
| Color Developing Reagent (TMB) | 10 mL |
| Stop Solution | 10 mL |
| Wash Buffer (25x) | 20 mL |
| Plate Sealers | 4 |
Also required (not supplied): microplate reader for 450 nm; incubator; automated plate washer (optional); precision pipettes for 0.5 µL through 1 mL volumes of aqueous solutions; multichannel pipettes (recommended for many samples); deionized or distilled water; 500 mL graduated cylinders; test tubes for dilution.
Performance Data
| Species | Human |
|---|---|
| Sample types | Cell culture supernatants, serum and plasma (heparin, EDTA) |
| Assay range | 156 pg/mL–10,000 pg/mL |
| Sensitivity | <75 pg/mL |
| Standard | Recombinant human IL23A ((R20-P189)+(I23-S328)), expressed in NS0 |
| Cross-reactivity | No significant cross-reactivity or interference with IL23A analogs in supplier testing; untested analogs cannot be excluded. |
Storage & Handling
Store at 4 °C for up to 6 months or −20 °C for up to 12 months. Avoid repeated freeze–thaw cycles. Ships on gel ice and tolerates up to 3 days at room temperature; freeze on arrival.
Safety
Sample data
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.009 | 0.11 | 0.203 | 0.348 | 0.568 | 0.975 | 1.495 | 2.001 |
Intra/inter assay consistency
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 280 | 519 | 4052 | 195 | 572 | 2736 |
| Standard deviation | 17.22 | 14.12 | 142.63 | 8.09 | 15.44 | 98.5 |
| CV(%) | 6.2% | 2.7% | 3.5% | 4.2% | 2.7% | 3.6% |
Kit components
Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4Materials required but not provided
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Maria D’Agostino et al. (2025) Protective and Anti-Inflammatory Effect of Novel Formulation Based on High and Low Molecular Weight Hyaluronic Acid and Salvia haenkei. International Journal of Molecular Sciences. 10.3390/ijms26031310
Das Dayasagar et al. (2023) Dominance and improved survivability of human γδT17 cell subset aggravates the immunopathogenesis of pemphigus vulgaris. Immunologic Research. 10.1007/s12026-023-09413-0
Haini Zhang et al. (2024) Upregulation of TLR2 in keratinocytes activates the MAPK pathway and plays a role in the pathogenesis of FHS. Journal of Dermatological Science. 10.1016/j.jdermsci.2024.11.002
Mamun-or-Rashid et al. (2024) Altered serum interleukin-17A and interleukin-23A levels may be associated with the pathophysiology and development of generalized anxiety disorder. Scientific Reports. 10.1038/s41598-024-66131-9
