| Field | Specification |
|---|---|
| Target | |
| Alternative names | Interleukin-3;IL-3;Hematopoietic growth factor;Mast cell growth factor;MCGF;Multipotential colony-stimulating factor;P-cell-stimulating factor;IL3; |
| Gene ID | |
| UniProt # | |
| Reactivity | |
| Applications | |
| Immunogen | Expression system for standard: E.coli; Immunogen sequence: A20-F152 |
| Expression system | |
| Expression region | |
| Assay type | |
| Sample type(s) | Cell Culture Supernatant, Serum, Plasma |
| Sensitivity | |
| Detection range | |
| Assay time | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Assay Principle
This sandwich ELISA quantifies human interleukin-3 (gene IL3) in cell culture supernatants, serum and plasma ( heparin, EDTA, citrate). Strips are pre-coated with a capture antibody. After the sample is added, a biotinylated detection antibody binds the captured protein, and an avidin–biotin–peroxidase complex supplies the enzyme. TMB develops color in proportion to the bound target; the reaction is stopped and read at 450 nm, and concentrations are read from the standard curve.
Interleukin-3 (IL-3) is a hematopoietic cytokine secreted mainly by activated T cells and mast cells. Through a receptor of IL-3Rα (CD123) and the common beta chain, it activates JAK2–STAT5 signaling to support the growth and differentiation of multiple blood cell progenitors and to activate basophils, eosinophils and monocytes.
Kit Components
| Anti-Human IL3 Pre-coated 96-well strip microplate | 1 plate (96 wells) |
|---|---|
| Human IL3 Standard | 2 vials, 10 ng/tube |
| Human IL3 Biotinylated antibody (100x) | 100 µL |
| Avidin-Biotin-Peroxidase Complex (100x) | 100 µL |
| Sample Diluent | 30 mL |
| Antibody Diluent | 12 mL |
| Avidin-Biotin-Peroxidase Diluent | 12 mL |
| Color Developing Reagent (TMB) | 10 mL |
| Stop Solution | 10 mL |
| Wash Buffer (25x) | 20 mL |
| Adhesive plate sealers | 4 |
Also required (not supplied): microplate reader for 450 nm; incubator; automated plate washer (optional); precision pipettes for 0.5 µL through 1 mL volumes of aqueous solutions; multichannel pipettes (recommended for many samples); deionized or distilled water; 500 mL graduated cylinders; test tubes for dilution.
Kit components are not sold separately.
Performance Data
| Species | Human |
|---|---|
| Sample types | Cell culture supernatants, serum and plasma ( heparin, EDTA, citrate) |
| Assay range | 15.6 pg/mL–1,000 pg/mL |
| Sensitivity | <1 pg/mL |
| Standard | Recombinant human IL3 (Ala20–Phe152), expressed in E. coli |
| Cross-reactivity | None detected against related proteins in supplier testing |
| Intra-assay CV | 4.7–7.6% (3 samples, n = 16 each) |
| Inter-assay CV | 4.7–7.6% (3 samples, n = 24 each) |
| Format | 96 wells, removable strips |
Sample dilution: start at 1:1. In the supplier’s tests, serum and plasma samples measured below the lowest standard.
Example standard curve (OD450, pg/mL): 0 = 0.027; 15.6 = 0.073; 31.2 = 0.108; 62.5 = 0.195; 125 = 0.353; 250 = 0.674; 500 = 1.188; 1000 = 2.014. Run a new standard curve on every plate.
Storage & Handling
Store at 4 °C for up to 6 months or −20 °C for up to 12 months. Avoid repeated freeze–thaw cycles. Ships on gel ice and tolerates up to 3 days at room temperature; freeze on arrival.
Safety
Sample data
| Concentration (pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
| O.D. | 0.027 | 0.073 | 0.108 | 0.195 | 0.353 | 0.674 | 1.188 | 2.014 |
Intra/inter assay consistency
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 33 | 205 | 387 | 31 | 190 | 374 |
| Standard deviation | 1.68 | 9.63 | 21.736 | 1.73 | 8.93 | 28.42 |
| CV (%) | 7.6% | 4.7% | 7.2% | 5.6% | 4.7% | 7.6% |
Kit components
Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4Materials required but not provided
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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McAlpine Cameron S. et al. (2021) Astrocytic interleukin-3 programs microglia and limits Alzheimer’s disease. Nature. 10.1038/s41586-021-03734-6
Nana Huang et al. (2026) IL-3 Modulates Microglia Polarization and Attenuates Neuroinflammation in Traumatic Brain Injury. Advanced Science. 10.1002/advs.202504511
Palma B Dalla et al. (2015) Osteolytic lesions, cytogenetic features and bone marrow levels of cytokines and chemokines in multiple myeloma patients: Role of chemokine (C-C motif) ligand 20. Leukemia. 10.1038/leu.2015.259
Akter Sarmin et al. (2023) Altered IL-3 and lipocalin-2 levels are associated with the pathophysiology of major depressive disorder: a case-control study. BMC Psychiatry. 10.1186/s12888-023-05354-y
Ran-Jing Tan et al. (2016) A 21–35 kDa Mixed Protein Component from Helicobacter pylori Activates Mast Cells Effectively in Chronic Spontaneous Urticaria. Helicobacter. 10.1111/hel.12312
Marina Bolzoni et al. (2012) Immunomodulatory drugs lenalidomide and pomalidomide inhibit multiple myeloma-induced osteoclast formation and the RANKL/OPG ratio in the myeloma microenvironment targeting the expression of adhesion molecules. Experimental Hematology. 10.1016/j.exphem.2012.11.005
