| Field | Specification |
|---|---|
| UniProt # | |
| Applications | |
| Sensitivity | |
| Detection range | |
| Detection method | |
| Assay time | |
| Storage | |
| Shipping | |
| Catalog no. (Mfr.) | |
| Main SKU |
Scientific Background
This ELISA kit applies to the in vitro quantitative determination of Human IL-33 concentrations in serum, plasma and other biological fluids.
Assay Principle
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IL-33. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IL-33 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IL-33, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human IL-33. You can calculate the concentration of Human IL-33 in the samples by comparing the OD of the samples to the standard curve.
Performance Specifications
| Sensitivity | 9.38 pg/mL |
|---|---|
| Detection Range | 15.63-1000 pg/mL |
| Total Assay Time | 3 h 30 min |
| Compatible Sample Types | Serum, plasma and other biological fluids |
| Species Reactivity | Human |
| Detection Method | Sandwich |
| Precision (CV) | Both intra-CV and inter-CV are < 10%. |
| Recovery Rate | 80%-120% |
| Storage | 2-8℃,12 months |
✓ Research-Grade Validation
Specificity
This kit recognizes Human IL-33 in samples. No significant cross-reactivity or interference between Human IL-33 and analogues was observed
Safety & Regulatory
Handle reagents in accordance with institutional biosafety guidelines. Refer to the Safety Data Sheet (SDS) for complete hazard and handling information. Contains components that may require special disposal procedures per local regulations.
This kit is validated for use with Serum, plasma and other biological fluids. For unlisted matrices (e.g., tissue lysate, urine), perform a spike-and-recovery experiment to confirm assay performance before generating reportable data. Sample dilution in the kit's provided diluent is recommended to minimize matrix interference.
The minimum detectable concentration (sensitivity) of this kit is 9.38 pg/mL. Values below this threshold should be reported as below the limit of detection (<LOD) and should not be extrapolated from the standard curve.
The total assay time from sample addition to absorbance reading is approximately 3 h 30 min, including all incubation, wash, and substrate steps. Hands-on time is typically 1–2 hours; most steps involve passive plate incubation. Plan the assay as a single uninterrupted session for best results.
Standard components of this Sandwich ELISA Kit typically include: pre-coated microplate (96-well strip format), lyophilized or liquid recombinant IL-33 standard, detection antibody, streptavidin-HRP conjugate, TMB substrate, stop solution, wash buffer concentrate, and sample/standard diluent. Refer to the kit insert or datasheet for the exact component list and storage requirements.
This kit uses colorimetric (TMB/HRP) detection and requires a standard microplate absorbance reader capable of measuring at 450 nm. A reference wavelength of 570 nm or 630 nm is recommended to reduce background. No specialized fluorescence or luminescence reader is needed. Ensure the instrument is calibrated and the plate is clean and free of condensation before reading.
Tissue samples may contain endogenous or exogenous proteases during processing, leading to degradation of extracted proteins. Therefore, it's necessary to add protease inhibitors during processing to ensure the integrity of target proteins. If customers can keep samples cold and handle them quickly during processing, omitting the protease inhibitor may not have a significant effect. After preparation, samples should be tested promptly or immediately aliquoted and frozen at -20°C or -80°C.
What is the range of enzyme activity of your IL-2 freeze-dried powder
What is the principle of adding stop solution to stop color reaction in ELISA experiment?
Can’t Find What You’re Looking For? We can help you source the best match or customize an ELISA solution for your study. Options may include alternative target synonyms, different species reactivity, sample type/matrix compatibility (serum/plasma/lysate/supernatant), assay format (sandwich/competitive), sensitivity/range, detection chemistry (colorimetric/fluorescent/chemiluminescent), plate format (pre-coated/uncoated, strips vs full plate), and bulk or custom packaging. Click Talk to a Scientist to submit a request form, email us at support@biohippo.com, or explore our Research Services for additional support. Our team will be in contact with you shortly.
OTUD6A in Airway Epithelial Cells Exacerbates Allergic Asthma by Promoting Airway Inflammation and Airway Remodeling Through Deubiquitination of hResistin/mRELMα (2026) Advanced Science. 10.1002/advs.202516355
A SCID mouse-human lung xenograft model of SARS-CoV-2 infection (2021) Theranostics. 10.7150/thno.58321
The glycocalyx of Lacticaseibacillus paracasei NPB01 presents two different rhamnose-rich polysaccharides with non-equivalent immunomodulating activities (2025) CARBOHYDRATE POLYMERS. 10.1016/j.carbpol.2025.123742
Sitravatinib Targets TYRO3 to Augment the Antitumor Immune Response of PD-1 Blockade in Hepatocellular Carcinoma (2025) CLINICAL CANCER RESEARCH. 10.1158/1078-0432.CCR-24-4338
Matrix-Bound EGF Promotes Malignant Phenotypes of Breast Cancer Organoids in the Biomimetic ECM of Alginate (2025) Materials Today Bio. 10.1016/j.mtbio.2025.101818
SUMOylated IL-33 in the nucleus stabilizes the transcription factor IRF1 in hepatocellular carcinoma cells to promote immune escape (2023) Science Signaling. 10.1126/scisignal.abq3362
Excess heme orchestrates progesterone resistance in uterine endometrial cancer through macrophage polarization and the IL-33/PAX8/PGR axis (2025) BIOMEDICINE & PHARMACOTHERAPY. 10.1016/j.biopha.2025.118008
Sappanone A Exerted Strong Anti-Inflammatory Effects in the Treatment of Atopic Dermatitis Through IL-21R-Mediated JAK1/STAT3 Pathway (2025) PHYTOTHERAPY RESEARCH. 10.1002/ptr.70123
The Alarmin Triad—IL-25, IL-33, and TSLP—Serum Levels and Their Clinical Implications in Chronic Spontaneous Urticaria (2024) INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES. 10.3390/ijms25042026
Relation of T Cell Profile with Vitamin D Receptor and Vitamin D-Binding Protein Gene Polymorphisms in Atopy (2024) INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES. 10.3390/ijms25169021
Molecular hydrogen alleviates asthma through inhibiting IL-33/ILC2 axis (2021) INFLAMMATION RESEARCH. 10.1007/s00011-021-01459-w
Profile of immune response during nasal challenge with dermatophagoides pteronyssinus in subjects with allergic airway diseases (2024) Journal of Inflammation-London. 10.1186/s12950-024-00415-9
Sulforaphane inhibits the migration and invasion of BPDE-induced lung adenocarcinoma cells by regulating NLRP12 (2024) TOXICOLOGY AND APPLIED PHARMACOLOGY. 10.1016/j.taap.2024.116916
Salivary IL-33 and sST2 levels in relation to TLR2 rs111200466 polymorphism and periodontitis (2023) ORAL DISEASES. 10.1111/odi.14675
Prognostic value of interleukin-33, sST2, myeloperoxidase, and matrix metalloproteinase-9 in acute aortic dissection (2023) Frontiers in Cardiovascular Medicine. 10.3389/fcvm.2022.1084321
Systemic and local cytokine profile and risk factors for persistent allergic airway inflammation in patients sensitised to house dust mite allergens (2021) BMC Pulmonary Medicine. 10.1186/s12890-021-01798-8
A role for serum cytokines and cell adhesion molecules in the non?invasive diagnosis of colorectal cancer (2022) Oncology Letters. 10.3892/ol.2022.13443
Serum Soluble ST2 and IL–33 Levels in Finnish Patients With Juvenile Idiopathic Arthritis (2025) APMIS. 10.1111/apm.70083
Anti-IgE Significantly Changes Circulating Interleukin-25, Vitamin-D and Interleukin-33 Levels in Patients with Allergic Asthma (2019) CURRENT PHARMACEUTICAL DESIGN. 10.2174/1381612825666190930095725
A case of asthma with Behcet’s disease: successful treatment with omalizumab and its effects on recurrent aphthous lesions (2020) IMMUNOPHARMACOLOGY AND IMMUNOTOXICOLOGY. 10.1080/08923973.2020.1789656
The function of IL-33/ST2 signaling axis in treg cells activating fibrosis in IgG4-related disease (2022) HUMAN IMMUNOLOGY. 10.1016/j.humimm.2022.01.009
FUT8 promotes HDM-induced glycolysis, epithelial-mesenchymal transition and inflammation in bronchial epithelial cells by inducing N-glycosylation modification of GLUT1 (2025) BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS. 10.1016/j.bbrc.2025.152721
Wheat‐dependent exercise‐induced anaphylaxis in Chinese people: a clinical research on 33 cases with antigenic analysis of wheat proteins (2020) CLINICAL AND EXPERIMENTAL DERMATOLOGY. 10.1111/ced.14035
Increased TSLP, IL-33, IL-25, IL-19, IL 21 and amphiregulin (AREG) levels in chronic rhinosinusitis with nasal polyp (2019) EUROPEAN ARCHIVES OF OTO-RHINO-LARYNGOLOGY. 10.1007/s00405-019-05379-8
Biomarkers of type 2 and non-type 2 inflammation in asthma exacerbations (2024) Central European Journal of Immunology. 10.5114/ceji.2024.141345
