| Field | Specification |
|---|---|
| Mfr No | |
| Assay Time | |
| Assay Type | |
| Product Type | |
| Sample Type(s) | cell culture supernatant, serum, plasma |
| Sensitivity | |
| Species | |
| Storage | |
| Target |
Interleukin-4 (IL4), is a secreted protein that belongs to the IL-4 / IL-13 family. IL-4 enhances both secretion and cell surface expression of IgE and IgG1. It regulates the expression of the low-affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. IL-4 is essential for the switching of B cells to IgE antibody production and for the maturation of T helper (Th) cells toward the Th2 phenotype. It participates in at least several B-cell activation processes.
Assay Principle
The Fast Human IL-4 ELISA is a solid phase ELISA designed to measure human IL-4 levels in cell culture supernatants, serum, and plasma.
Key Features Novel Proprietary ELISA Approach
Simple: Sample and Detection combined into one step.
Human IL-4 ELISA Kit is a Solid-phase sandwich ELISA for the quantitative determination of IL-4. The microplate is pre-coated with a capture antibody specific to the target. Target present in the sample or standard binds the immobilized antibody, is detected with a biotinylated detection antibody and streptavidin-HRP, and quantified colorimetrically against a standard curve.
Kit Components
The kit provides a pre-coated microplate, target standard for standard-curve generation, detection reagents (detection antibody and enzyme conjugate), wash buffer, substrate, and stop solution. Refer to the product datasheet for the complete component list and quantities.
Performance Data
| Target | IL-4 |
|---|---|
| Species | Human |
| Sample Type | cell culture supernatant, serum, plasma |
| Assay Type | Sandwich |
| Test Principle | Solid-phase sandwich ELISA |
| Sensitivity | 8.0 pg/mL |
| Assay Time | 1 hour |
| Specificity | This assay recognizes natural and recombinant human IL-4. |
| Storage | Store the unopened kit at 2-8°C. | Components: The unused wells can be stored in the sealed foil pouch containing the desiccant pack for up to 1 month at 2-8°C.; Aliquot and store at -20°C for up to 1 month in a manual defrost freezer. Avoid repeated freeze-thaw cycles.; May be stored for up to 3 months at 2-8°C. |
✓ Quality tested for reproducibility and lot-to-lot consistency
Safety & Handling
This kit is validated for cell culture supernatant, serum, plasma. Other matrices may require additional in-lab validation.
This assay has a sensitivity of 8.0 pg/mL. Samples reading outside the range should be diluted or concentrated.
The total assay time is approximately 1 hour from sample addition to plate reading.
The kit provides a pre-coated microplate, target standard for standard-curve generation, detection reagents (detection antibody and enzyme conjugate), wash buffer, substrate, and stop solution. See the datasheet for the full component list and quantities.
A standard microplate reader capable of measuring absorbance at 450 nm is required for this sandwich ELISA.
Customization & Add-ons: Need this ELISA kit tailored to your workflow? We can help with bulk or custom pack sizes, species or analyte variants, matched antibody pairs and standards, alternate plate formats (48- or 96-well, strip-well), and assay development or custom ELISA solutions for targets not in our catalog. Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services — our team will follow up with feasibility details and next steps.
