| Field | Specification |
|---|---|
| Target | |
| Alternative names | Interleukin-5;IL-5;B-cell differentiation factor I;Eosinophil differentiation factor;T-cell replacing factor;TRF;IL5; |
| Gene ID | |
| UniProt # | |
| Reactivity | |
| Applications | |
| Immunogen | Expression system for standard: NS0; Immunogen sequence: I20-S134 |
| Expression system | |
| Expression region | |
| Assay type | |
| Sample type(s) | Cell Culture Supernatant, Serum, Plasma, Urine |
| Sensitivity | |
| Detection range | |
| Assay time | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Assay Principle
This sandwich ELISA quantifies human interleukin-5 (gene IL5) in cell culture supernatants, serum, plasma (heparin, EDTA) and urine. Strips are pre-coated with a capture antibody. After the sample is added, a biotinylated detection antibody binds the captured protein, and an avidin–biotin–peroxidase complex supplies the enzyme. TMB develops color in proportion to the bound target; the reaction is stopped and read at 450 nm, and concentrations are read from the standard curve.
Interleukin-5 (IL-5) is a homodimeric cytokine produced mainly by Th2 cells and group 2 innate lymphoid cells. Through a receptor of IL-5Rα and the common beta chain, it activates JAK2–STAT5 and RAS–MAPK signaling to control eosinophil production, survival and migration, making it central to research on eosinophilic inflammation and asthma.
Kit Components
| Anti-Human IL5 Pre-coated 96-well strip microplate | 1 plate (96 wells) |
|---|---|
| Human IL5 Standard | 2 vials, 10 ng/tube |
| Human IL5 Biotinylated antibody (100x) | 100 µL |
| Avidin-Biotin-Peroxidase Complex (100x) | 100 µL |
| Sample Diluent | 30 mL |
| Antibody Diluent | 12 mL |
| Avidin-Biotin-Peroxidase Diluent | 12 mL |
| Color Developing Reagent (TMB) | 10 mL |
| Stop Solution | 10 mL |
| Wash Buffer (25x) | 20 mL |
| Adhesive plate sealers | 4 |
Also required (not supplied): microplate reader for 450 nm; incubator; automated plate washer (optional); precision pipettes for 0.5 µL through 1 mL volumes of aqueous solutions; multichannel pipettes (recommended for many samples); deionized or distilled water; 500 mL graduated cylinders; test tubes for dilution.
Kit components are not sold separately.
Performance Data
| Species | Human |
|---|---|
| Sample types | Cell culture supernatants, serum, plasma (heparin, EDTA) and urine |
| Assay range | 7.8 pg/mL–500 pg/mL |
| Sensitivity | <2 pg/mL |
| Standard | Recombinant human IL5 (Ile20–Ser134), expressed in NS0 |
| Cross-reactivity | None detected against related proteins in supplier testing |
| Intra-assay CV | 6.4–7.8% (3 samples, n = 16 each) |
| Inter-assay CV | 6.5–9.3% (3 samples, n = 24 each) |
| Format | 96 wells, removable strips |
Sample dilution: start at 1:1. In the supplier’s tests, serum and plasma samples measured below the lowest standard.
Example standard curve (OD450, pg/mL): 0 = 0.041; 7.8 = 0.083; 15.6 = 0.148; 31.3 = 0.268; 62.5 = 0.469; 125 = 0.943; 250 = 1.483; 500 = 1.982. Run a new standard curve on every plate.
Storage & Handling
Store at 4 °C for up to 6 months or −20 °C for up to 12 months. Avoid repeated freeze–thaw cycles. Ships on gel ice and tolerates up to 3 days at room temperature; freeze on arrival.
Safety
Sample data
| Concentration (pg/ml) | 0 | 7.8 | 15.6 | 31.3 | 62.5 | 125 | 250 | 500 |
| O.D. | 0.041 | 0.083 | 0.148 | 0.268 | 0.469 | 0.943 | 1.483 | 1.982 |
Intra/inter assay consistency
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 9 | 73 | 238 | 8 | 70 | 234 |
| Standard deviation | 0.7 | 4.67 | 16.42 | 0.74 | 4.55 | 20.12 |
| CV (%) | 7.8% | 6.4% | 6.9% | 9.3% | 6.5% | 8.6% |
Kit components
Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4Materials required but not provided
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Liu Tingting et al. (2024) Single-cell transcriptomics analysis of bullous pemphigoid unveils immune-stromal crosstalk in type 2 inflammatory disease. Nature Communications. 10.1038/s41467-024-50283-3
Yi-Giien Tsai et al. (2019) Increased Type 2 Innate Lymphoid Cells in Patients with Drug Reaction with Eosinophilia and Systemic Symptoms Syndrome. Journal of Investigative Dermatology. 10.1016/j.jid.2018.10.048
Qifeng Li et al. (2021) ILC2s induce adaptive Th2-type immunity in different stages of tuberculosis through the Notch-GATA3 pathway. International Immunopharmacology. 10.1016/j.intimp.2021.108330
Yishan Xiong et al. (2026) A prospective cohort study of ultra-rush subcutaneous immunotherapy in dust mite-induced allergic rhinitis. World Allergy Organization Journal. 10.1016/j.waojou.2026.101334
Zhen Tang et al. (2017) Peripheral proinflammatory cytokines in Chinese patients with generalised anxiety disorder. Journal of Affective Disorders. 10.1016/j.jad.2017.08.082
Wei Qing et al. (2021) Relationship between Th17-mediated immunity and airway inflammation in childhood neutrophilic asthma. Allergy Asthma and Clinical Immunology. 10.1186/s13223-020-00504-3
Wen Cuiju et al. (2020) IL-2 and IL-2R gene polymorphisms and immune function in people residing in areas with high background radiation, Yangjiang, China. International Journal of Radiation Biology. 10.1080/09553002.2020.1820607
Wu Hong et al. (2022) Proportion of natural killer cells in peripheral blood lymphocytes is correlated with cytokine levels in patients with type 2 diabetes mellitus and prediabetes: a preliminary report. International Journal of Diabetes in Developing Countries. 10.1007/s13410-022-01069-0
