| Field | Specification |
|---|---|
| Target | |
| Alternative names | Interleukin 6 receptor ;Interleukin 6 receptor, isoform CRA_a ;IL6R ;hCG_1810911 ; |
| Gene ID | |
| UniProt # | |
| Reactivity | |
| Applications | |
| Immunogen | Expression system for standard: NS0; Immunogen sequence: L20-D358 |
| Expression system | |
| Expression region | |
| Assay type | |
| Sample type(s) | Cell Culture Supernatant, Serum, Plasma, Urine |
| Sensitivity | |
| Detection range | |
| Assay time | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Assay Principle
This sandwich ELISA quantifies human interleukin-6 receptor subunit alpha (gene IL6R) in cell culture supernatants, serum, plasma (heparin, EDTA, citrate) and urine. Strips are pre-coated with a capture antibody. After the sample is added, a biotinylated detection antibody binds the captured protein, and an avidin–biotin–peroxidase complex supplies the enzyme. TMB develops color in proportion to the bound target; the reaction is stopped and read at 450 nm, and concentrations are read from the standard curve.
The IL-6 receptor alpha chain (IL-6R, CD126) binds IL-6 but needs gp130 (IL6ST) to transmit signals. The membrane form is limited to hepatocytes and some immune cells, while a soluble form allows IL-6 to act on gp130-expressing cells through trans-signaling. It is studied in inflammation, acute phase responses and hematopoiesis.
Kit Components
| Anti-Human IL6R Pre-coated 96-well strip microplate | 1 plate (96 wells) |
|---|---|
| Human IL6R Standard | 2 vials, 10 ng/tube |
| Human IL6R Biotinylated antibody (100x) | 100 µL |
| Avidin-Biotin-Peroxidase Complex (100x) | 100 µL |
| Sample Diluent | 30 mL |
| Antibody Diluent | 12 mL |
| Avidin-Biotin-Peroxidase Diluent | 12 mL |
| Color Developing Reagent (TMB) | 10 mL |
| Stop Solution | 10 mL |
| Wash Buffer (25x) | 20 mL |
| Adhesive plate sealers | 4 |
Also required (not supplied): microplate reader for 450 nm; incubator; automated plate washer (optional); precision pipettes for 0.5 µL through 1 mL volumes of aqueous solutions; multichannel pipettes (recommended for many samples); deionized or distilled water; 500 mL graduated cylinders; test tubes for dilution.
Kit components are not sold separately.
Performance Data
| Species | Human |
|---|---|
| Sample types | Cell culture supernatants, serum, plasma (heparin, EDTA, citrate) and urine |
| Assay range | 31.2 pg/mL–2,000 pg/mL |
| Sensitivity | <10 pg/mL |
| Standard | Recombinant human IL6R (Leu20–Asp358), expressed in NS0 |
| Cross-reactivity | None detected against related proteins in supplier testing |
| Intra-assay CV | 5.3–6.7% (3 samples, n = 16 each) |
| Inter-assay CV | 5.7–6.7% (3 samples, n = 24 each) |
| Format | 96 wells, removable strips |
Sample dilution: start at 1:100. In the supplier’s tests, serum and plasma samples measured 10837–53930 pg/mL.
Example standard curve (OD450, pg/mL): 0 = 0.031; 31.2 = 0.127; 62.5 = 0.21; 125 = 0.356; 250 = 0.613; 500 = 1.012; 1000 = 1.615; 2000 = 2.39. Run a new standard curve on every plate.
Storage & Handling
Store at 4 °C for up to 6 months or −20 °C for up to 12 months. Avoid repeated freeze–thaw cycles. Ships on gel ice and tolerates up to 3 days at room temperature; freeze on arrival.
Safety
Sample data
| Concentration (pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
| O.D. | 0.031 | 0.127 | 0.21 | 0.356 | 0.613 | 1.012 | 1.615 | 2.39 |
Intra/inter assay consistency
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 54 | 271 | 821 | 59 | 259 | 773 |
| Standard deviation | 2.86 | 17.07 | 41.87 | 3.77 | 17.35 | 44.06 |
| CV (%) | 5.3% | 6.7% | 5.7% | 6.4% | 6.7% | 5.7% |
Kit components
Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4Materials required but not provided
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Zhou Yang et al. (2021) Beta-Lapachone Attenuates BMSC-Mediated Neuroblastoma Malignant Transformation by Inhibiting Gal-3/Gal-3BP/IL6 Axis. Frontiers in Pharmacology. 10.3389/fphar.2021.766909
Chen Guojie et al. (2024) NUSAP1 Promotes Immunity and Apoptosis by the SHCBP1/JAK2/STAT3 Phosphorylation Pathway to Induce Dendritic Cell Generation in Hepatocellular Carcinoma. Journal of Immunotherapy. 10.1097/CJI.0000000000000531
