| Field | Specification |
|---|---|
| Target | |
| Alternative names | Interleukin-8;IL-8;C-X-C motif chemokine 8;Chemokine (C-X-C motif) ligand 8;Emoctakin;Granulocyte chemotactic protein 1;GCP-1;Monocyte-derived neutrophil chemotactic factor;MDNCF;Monocyte-derived neutrophil-activating peptide;MONAP;Neutrophil-activating protein 1;NAP-1;Protein 3-10C;T-cell chemotactic factor;MDNCF-a;GCP/IL-8 protein IV;IL8/NAP1 form I;Interleukin-8; (Ala-IL-8)77;GCP/IL-8 protein II;IL-8 (1-77);IL8/NAP1 form II;MDNCF-b;IL-8 (5-77);IL-8 (6-77); (Ser-IL-8)72;GCP/IL-8 protein I;IL8/NAP1 form III;Lymphocyte-derived neutrophil-activating factor;LYNAP;MDNCF-c;Neutrophil-activating factor;NAF;IL-8 (7-77);GCP/IL-8 protein V;IL8/NAP1 form IV;IL-8 (8-77);GCP/IL-8 protein VI;IL8/NAP1 form V;IL-8 (9-77);GCP/IL-8 protein III;IL8/NAP1 form VI;CXCL8;IL8; |
| Gene ID | |
| UniProt # | |
| Reactivity | |
| Applications | |
| Immunogen | Expression system for standard: E.coli; Immunogen sequence: S28-S99 |
| Expression system | |
| Expression region | |
| Assay type | |
| Sample type(s) | Cell Culture Supernatant, Serum, Plasma |
| Sensitivity | |
| Detection range | |
| Assay time | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Assay Principle
This sandwich ELISA quantifies human interleukin-8 (gene CXCL8) in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Strips are pre-coated with a capture antibody. After the sample is added, a biotinylated detection antibody binds the captured protein, and an avidin–biotin–peroxidase complex supplies the enzyme. TMB develops color in proportion to the bound target; the reaction is stopped and read at 450 nm, and concentrations are read from the standard curve.
Interleukin-8 (IL-8, CXCL8) is a CXC chemokine released by macrophages, epithelial and endothelial cells in response to inflammatory signals. Through the receptors CXCR1 and CXCR2 it attracts and activates neutrophils and supports angiogenesis. IL-8 is one of the most commonly measured inflammatory cytokines in infection, inflammation and cancer research. Rodents lack a direct ortholog.
Kit Components
| Anti-Human CXCL8 Pre-coated 96-well strip microplate | 1 plate (96 wells) |
|---|---|
| Human CXCL8 Standard | 2 vials, 10 ng/tube |
| Human CXCL8 Biotinylated antibody (100x) | 200 µL |
| Avidin-Biotin-Peroxidase Complex (100x) | 200 µL |
| Sample Diluent | 30mlx2 |
| Antibody Diluent | 25 mL |
| Avidin-Biotin-Peroxidase Diluent | 25 mL |
| Color Developing Reagent (TMB) | 20 mL |
| Stop Solution | 10 mL |
| Wash Buffer (25x) | 20 mL |
| Adhesive plate sealers | 4 |
Also required (not supplied): microplate reader for 450 nm; incubator; automated plate washer (optional); precision pipettes for 0.5 µL through 1 mL volumes of aqueous solutions; multichannel pipettes (recommended for many samples); deionized or distilled water; 500 mL graduated cylinders; test tubes for dilution.
Kit components are not sold separately.
Performance Data
| Species | Human |
|---|---|
| Sample types | Cell culture supernatants, serum and plasma (heparin, EDTA, citrate) |
| Assay range | 1.56–100 pg/mL |
| Sensitivity | <0.19 pg/mL |
| Standard | Recombinant human CXCL8 (Ser28–Ser99), expressed in E. coli |
| Cross-reactivity | None detected against related proteins in supplier testing |
| Intra-assay CV | 2.5–3.7% (3 samples, n = 16 each) |
| Inter-assay CV | 2.4–3.4% (3 samples, n = 24 each) |
| Format | 96 wells, removable strips |
Example standard curve (OD450, pg/mL): 0 = 0.017; 1.56 = 0.066; 3.12 = 0.105; 6.25 = 0.166; 12.5 = 0.298; 25 = 0.592; 50 = 1.082; 100 = 1.945. Run a new standard curve on every plate.
Storage & Handling
Store at 4 °C for up to 6 months or −20 °C for up to 12 months. Avoid repeated freeze–thaw cycles. Ships on gel ice and tolerates up to 3 days at room temperature; freeze on arrival.
Safety
Sample data
| Concentration (pg/ml) | 0 | 1.56 | 3.12 | 6.25 | 12.5 | 25 | 50 | 100 |
| O.D. | 0.017 | 0.066 | 0.105 | 0.166 | 0.298 | 0.592 | 1.082 | 1.945 |
Intra/inter assay consistency
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 3 | 10 | 30 | 3 | 7 | 29 |
| Standard deviation | 0.08 | 0.37 | 0.94 | 0.08 | 0.17 | 0.98 |
| CV (%) | 2.5% | 3.7% | 3.1% | 2.8% | 2.4% | 3.4% |
Kit components
Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4Materials required but not provided
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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