Human IL1b(Interleukin 1 Beta) ELISA Kit

SKU:BHE15204121
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Overview
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Quantitative sandwich ELISA kit for detecting Human IL1b (Interleukin 1 Beta) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. Detection range: 15.63-1000 pg/mL; Sensitivity/LoD: 5.8 pg/mL.
Target IL1b
Reactivity Human
Sample Type(s) serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Type Sandwich
Sensitivity 5.8 pg/mL
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Options selector
Catalog no. Size
ELK1270-96T 96 T
ELK1270-48T 48 T
ELK1270-96TX5 96 T X 5
Available Options
  • Please chose the Right size for your study: Choose 1×48T for pilot runs or limited samples, 1×96T for standard studies, or 5×96T for screening/high throughput.
  • Replicates & controls: Confirm you’ll have enough wells for standards, blanks, positive/negative controls, and technical replicates.
  • Timeline planning: these kits may require 5–7 business days lead to delivery.

If you’re unsure which format fits your sample count or workflow, contact us before ordering.

Field Specification
Mfr No ELK1270
Alternative Names IL-1B;IL1-B; IL1-Beta; IL1F2; IL-1β; Interleukin-1 Family Member 2; Catabolin
Assay Time
  • 3.5h
Assay Type
  • Sandwich
Detection Range 15.63-1000 pg/mL
Product Type
  • ELISA Kits
Reactivity
  • Human
Sample Type(s) serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity 5.8 pg/mL
Target IL1b
UniProt # P01584

Scientific background

IL1b (Interleukin 1 Beta) is an interleukin-family cytokine that helps coordinate immune cell communication and inflammatory responses.

Interleukin levels often shift quickly after immune stimulation, making them useful for time-course studies of inflammation and immune activation.

Quantifying interleukins can help interpret pathway engagement alongside phenotypic readouts (e.g., immune cell infiltration, fever, or tissue injury markers).

Why it matters

  • Quantify IL1b (Interleukin 1 Beta) to compare biological changes across conditions, doses, or time points.
  • Generate concentration data from a standard curve to support biomarker and mechanistic studies.

How the ELISA works

Designed for Human samples, this kit uses a The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IL1b. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human IL1b. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human IL1b, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IL1b in the samples is then determined by comparing the OD of the samples to the standard curve.. After binding and washing, signal is converted to concentration using a standard curve.

Sample types: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

  • Detection range: 15.63-1000 pg/mL
  • Sensitivity/LoD: 5.8 pg/mL
  • Assay time: 3.5h
▶Is there a reward for publishing papers using ELK ELISA Kit?
If you use our ELISA Kit and have published a paper, you can get a reward of up to 300 USD, please contact our sales staff for the policy. Reward table (by IF): 2 ó (IF) < 5 ? 50 USD 5 ó (IF) < 10 ? 100 USD 10 ó (IF) < 15 ? 200 USD (IF) ò 15 ? 300 USD
▶What is the storage temperature of the kit after receipt? Can it be placed at -20øC?
Unopened kit can be stored at 4ø C 6 months; -20ø C 12 months
▶How about the accuracy and stability of your kits? How long is the shelf life? There won't be any problem during transportation, right?
The accuracy and stability of our kits are very good, all the products have been verified by thermal stability experiments in the R&D stage, and must undergo a three steps quality check before shipment, and the special stabilizers developed by ELK can well guarantee the activity of each reagent. The validity period is 4ø C 6 months, -20ø C 12 months, to ensure that the reagent kit 4 degrees transportation is safer and more stable
▶What is the purification method of the antibody in the kit?
Affinity chromatography
▶Can your kits be used for clinical diagnosis?
Research use only, not for clinical diagnosis.
▶How many samples can be tested in one kit?
96T Standard curve: NO Duplicatewells; Samples: NO Duplicatewells ? Tested Samples: 88; EasyStep method: 96 Standard curve: Duplicate wells; Samples: NO Duplicatewells ? Tested Samples: 80; EasyStep method: 96 Standard curve: NO Duplicatewells; Samples: Duplicatewells ? Tested Samples: 44; EasyStep method: 48 Standard curve: Duplicate wells; Samples: Duplicatewells ? Tested Samples: 40; EasyStep method: 48 48T Standard curve: NO Duplicatewells; Samples: NO Duplicatewells ? Tested Samples: 40; EasyStep method: 48 Standard curve: Two Duplicatewells; Samples: NO Duplicatewells ? Tested Samples: 32; EasyStep method: 48 Standard curve: NO Duplicatewells; Samples: Two Duplicatewells ? Tested Samples: 20; EasyStep method: 24 Standard curve: Two Duplicatewells; Samples: Two Duplicatewells ? Tested Samples: 16; EasyStep method: 24
▶How should the standard be stored after dissolution? 4?? How long can it be stored after dissolution?
The standard should be preparsd right before use, and used as soon as possible within 24 hours after dissolution to avoid inaccurate quantification due to partial degradation. The stock solution can be stored until -20 and then used within one week, other concentrations of liquid can not be stored, the next timeyou use the liquid need to be re-diluted from the stock solution
▶Can you provide a more concentrated standard so that the detection range can cover the concentration of my sample?
The maximum value and detection range of the standards in the kit are determined by the assay data at the R&D stage and cannot be changed arbitrarily. If the sample concentration is too high, it can be diluted. The dilution ratio can be determined by pre-test. Generally speaking, if the sample concentration is too high, the detection can be completed by dilution. If there is a definite need for a kit with the appropriate range and sensitivity, customized services are available.
▶I don't know how many times my sample should be diluted? Can you provide me with a detailed description?
The dilution should be determined by pre-experiment (for the accuracy of the experiment, make a prediction of the target protein content of the sample first. Then compare the detection range of the kit, if it exceeds the corresponding dilution, it is recommended to take 2-3 samples, each sample to do a gradient dilution, such as (stock solution, 1:5, 1:20), the detection of the sample OD in the range of the standard curve S2_54, for the optimal dilution of the sample)
▶Can saline be used for sample dilution?
In order to ensure that all wells are in the same condition, please use the sample diluent provided in the kit, not saline or other self-prepared reagents when diluting the samples.
▶Can this kit be used to test cell lysate? If yes, is it possible to use the lysate directly, or do we need to separate the nucleus part of the cell?
Cell lysate can be detected, and the supernatant can be centrifuged directly after cell lysis.
▶How should the data be calculated after the experiment?
Reply: The data measured in the experiment can be imported into the professional curve software CurveExpert1.38 or 1.4, which can help you automatically match a most suitable curve. Please refer to the technical support article in our website for the specific operation steps.
▶How much sample volume do I need to prepare?
Reply: Generally, 100ul is required, 200ul is required for 1 duplicate well and so on (only 50ul is required for the competition method). If it is determined that the sample needs to be diluted a certain number of times, the sample amount can be reducedaccordingly. For ELISA kits using the sandwich principle, a sample volume of 100ul is required for a single-well test; for ELISA kits using the competition principle, a sample volume of 50ug is required for a single-well test; in addition, we have also launched a version of the micro-volume sampling method, which requires a sample volume of only 25ul for a single-well test, please feel free to contact us.
▶Concentrated biotinylated antibody and HRP enzyme conjugate are not enough?
The reagents in the kit are shipped in sufficient quantities in consideration of packaging and usage losses. so there is no risk of running out of reagents for normal use. Before use, centrifugation is required to minimize the loss of tube wall adhesion, and because multiple experiments will Increase the loss, it is necessary to pay attention to the control of loss when using in batches.

Can?t Find What You?re Looking For? We can help you source the best match or customize an ELISA solution for your study. Options may include alternative target synonyms, different species reactivity, sample type/matrix compatibility (serum/plasma/lysate/supernatant), assay format (sandwich/competitive), sensitivity/range, detection chemistry (colorimetric/fluorescent/chemiluminescent), plate format (pre-coated/uncoated, strips vs full plate), and bulk or custom packaging. Click Talk to a Scientist to submit a request form, email us at support@biohippo.com, or explore our Research Services for additional support. Our team will be in contact with you shortly.

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Can your kits be used for clinical diagnosis?
Research use only, not for clinical diagnosis.
I don't know how many times my sample should be diluted? Can you provide me with a detailed description?
The dilution should be determined by pre-experiment (for the accuracy of the experiment, make a prediction of the target protein content of the sample first. Then compare the detection range of the kit, if it exceeds the corresponding dilution, it is recommended to take 2-3 samples, each sample to do a gradient dilution, such as (stock solution, 1:5, 1:20), the detection of the sample OD in the range of the standard curve S2_54, for the optimal dilution of the sample)
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