| Field | Specification |
|---|---|
| Target | |
| Alternative names | Lymphocyte activation gene 3 protein; LAG-3; CD antigen CD223; LAG3; FDC |
| Gene ID | |
| UniProt # | |
| Reactivity | |
| Applications | |
| Immunogen | Expression system for standard: CHO; Immunogen sequence: Leu23-Leu450 |
| Assay type | |
| Sample type(s) | Cell Culture Supernatant, Serum, Plasma |
| Sensitivity | |
| Detection range | |
| Assay time | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Assay Principle
This sandwich ELISA quantifies human lymphocyte activation gene 3 protein (gene LAG3) in cell culture supernatants, serum and plasma (heparin, EDTA). Strips are pre-coated with a capture antibody. After the sample is added, a biotinylated detection antibody binds the captured protein, and an avidin–biotin–peroxidase complex supplies the enzyme. TMB develops color in proportion to the bound target; the reaction is stopped and read at 450 nm, and concentrations are read from the standard curve.
LAG-3 (CD223) is an inhibitory immune checkpoint receptor expressed on activated T cells and some other lymphocytes. Binding to MHC class II on antigen-presenting cells, or to FGL1, dampens T-cell receptor signaling and limits T-cell proliferation and effector function. LAG-3 often acts together with PD-1, and it is widely studied in T-cell exhaustion, autoimmunity and cancer immunotherapy.
Kit Components
| Anti-Human LAG3 Pre-coated 96-well Strip Microplate | 12 strips of 8 wells |
|---|---|
| Human LAG3 Standard | 2 × 10 ng/tube |
| Human LAG3 Biotinylated Antibody (100x) | 100 µL |
| Avidin-Biotin-Peroxidase Complex (100x) | 100 µL |
| Sample Diluent | 30 mL |
| Antibody Diluent | 12 mL |
| Avidin-Biotin-Peroxidase Diluent | 12 mL |
| Color Developing Reagent (TMB) | 10 mL |
| Stop Solution | 10 mL |
| Wash Buffer (25x) | 20 mL |
| Plate Sealers | 4 |
Also required (not supplied): microplate reader for 450 nm; incubator; automated plate washer (optional); precision pipettes for 0.5 µL through 1 mL volumes of aqueous solutions; multichannel pipettes (recommended for many samples); deionized or distilled water; 500 mL graduated cylinders; test tubes for dilution.
Performance Data
| Species | Human |
|---|---|
| Sample types | Cell culture supernatants, serum, plasma (heparin, EDTA) |
| Assay range | 93.8–6,000 pg/mL |
| Sensitivity | <15 pg/mL |
| Standard | Recombinant human LAG3 (Leu23–Leu450), expressed in CHO |
| Cross-reactivity | No significant cross-reactivity or interference with LAG3 analogs in supplier testing; untested analogs cannot be excluded. |
Storage & Handling
Store at 4 °C for up to 6 months or −20 °C for up to 12 months. Avoid repeated freeze–thaw cycles. Ships on gel ice and tolerates up to 3 days at room temperature; freeze on arrival.
Safety
Sample data
| Concentration (pg/ml) | 0 | 93.8 | 187.5 | 375 | 750 | 1500 | 3000 | 6000 |
| O.D. | 0.051 | 0.109 | 0.159 | 0.281 | 0.513 | 0.923 | 1.608 | 2.329 |
Intra/inter assay consistency
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 100 | 622 | 2155 | 126 | 601 | 2836 |
| Standard deviation | 2.9 | 16.86 | 93.31 | 9.32 | 24.28 | 109.47 |
| CV (%) | 2.9% | 2.7% | 4.3% | 7.4% | 4.0% | 3.9% |
Kit components
Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4Can’t Find What You’re Looking For? We can help you source the best match or customize an ELISA solution for your study. Options may include alternative target synonyms, different species reactivity, sample type/matrix compatibility (serum/plasma/lysate/supernatant), assay format (sandwich/competitive), sensitivity/range, detection chemistry (colorimetric/fluorescent/chemiluminescent), plate format (pre-coated/uncoated, strips vs full plate), and bulk or custom packaging. Click Talk to a Scientist to submit a request form, email us at support@biohippo.com, or explore our Research Services for additional support. Our team will be in contact with you shortly.
