| Field | Specification |
|---|---|
| Target | |
| Alternative names | Transforming growth factor beta-1;TGF-beta-1;Latency-associated peptide;LAP;TGFB1;TGFB; |
| Gene ID | |
| UniProt # | |
| Reactivity | |
| Applications | |
| Immunogen | Expression system for standard: NS0; Immunogen sequence: L30-R278 |
| Expression system | |
| Expression region | |
| Assay type | |
| Sample type(s) | Cell Culture Supernatant, Cell Lysate, Serum, Plasma |
| Sensitivity | |
| Detection range | |
| Assay time | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Assay Principle
This sandwich ELISA quantifies human transforming growth factor beta-1 proprotein (gene TGFB1) in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Strips are pre-coated with a capture antibody. After the sample is added, a biotinylated detection antibody binds the captured protein, and an avidin–biotin–peroxidase complex supplies the enzyme. TMB develops color in proportion to the bound target; the reaction is stopped and read at 450 nm, and concentrations are read from the standard curve.
TGF-β1 is the prototypical member of the TGF-β family of cytokines. It is secreted in a latent form held by its latency-associated peptide and, once activated, signals through TGFBR1/TGFBR2 and SMAD proteins to control cell growth, differentiation, extracellular matrix production and immune tolerance, making it central to fibrosis, immunology and cancer research.
Kit Components
| Anti-Human LAP(TGF-Beta1) Pre-coated 96-well strip microplate | 1 plate (96 wells) |
|---|---|
| Human LAP(TGF-Beta1) Standard | 2 vials, 10 ng/tube |
| Human LAP(TGF-Beta1) Biotinylated antibody (100x) | 100 µL |
| Avidin-Biotin-Peroxidase Complex (100x) | 100 µL |
| Sample Diluent | 30 mL |
| Antibody Diluent | 12 mL |
| Avidin-Biotin-Peroxidase Diluent | 12 mL |
| Color Developing Reagent (TMB) | 10 mL |
| Stop Solution | 10 mL |
| Wash Buffer (25x) | 20 mL |
| Adhesive plate sealers | 4 |
Also required (not supplied): microplate reader for 450 nm; incubator; automated plate washer (optional); precision pipettes for 0.5 µL through 1 mL volumes of aqueous solutions; multichannel pipettes (recommended for many samples); deionized or distilled water; 500 mL graduated cylinders; test tubes for dilution.
Kit components are not sold separately.
Performance Data
| Species | Human |
|---|---|
| Sample types | Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) |
| Assay range | 62.5 pg/mL–4,000 pg/mL |
| Sensitivity | <10 pg/mL |
| Standard | Recombinant human TGFB1 (Leu30–Arg278), expressed in NS0 |
| Cross-reactivity | None detected against related proteins in supplier testing |
| Intra-assay CV | 4.5–8.3% (3 samples, n = 16 each) |
| Inter-assay CV | 5.0–8.3% (3 samples, n = 24 each) |
| Format | 96 wells, removable strips |
Sample dilution: start at 1:100. In the supplier’s tests, serum and plasma samples measured about 80 ng/mL.
Example standard curve (OD450, pg/mL): 0 = 0.052; 62.5 = 0.129; 125 = 0.186; 250 = 0.272; 500 = 0.471; 1000 = 0.78; 2000 = 1.245; 4000 = 1.936. Run a new standard curve on every plate.
Storage & Handling
Store at 4 °C for up to 6 months or −20 °C for up to 12 months. Avoid repeated freeze–thaw cycles. Ships on gel ice and tolerates up to 3 days at room temperature; freeze on arrival.
Safety
Sample data
| Concentration (pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | 4000 |
| O.D. | 0.052 | 0.129 | 0.186 | 0.272 | 0.471 | 0.78 | 1.245 | 1.936 |
Intra/inter assay consistency
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 87 | 673 | 2000 | 90 | 698 | 2085 |
| Standard deviation | 3.91 | 43.74 | 126 | 4.5 | 57.93 | 148.35 |
| CV (%) | 4.5% | 6.5% | 8.3% | 5% | 8.3% | 7% |
Kit components
Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4Materials required but not provided
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Activating reagent preparation
LAP (TGF-beta1) is mostly contained as inactive form in samples, please activate it before assay. Don’t activate recombinant LAP (TGF-beta1). Solution A: 1N HCI: add 8.33ml of 12N HCI into 91.67ml of H2O. Solution B: 1.2N NaOH/0.5M HEPES: add 12ml of 10N NaOH and 11.9g HEPES into 75ml of H2O, add H2O to adjust volume to 100ml.
Can’t Find What You’re Looking For? We can help you source the best match or customize an ELISA solution for your study. Options may include alternative target synonyms, different species reactivity, sample type/matrix compatibility (serum/plasma/lysate/supernatant), assay format (sandwich/competitive), sensitivity/range, detection chemistry (colorimetric/fluorescent/chemiluminescent), plate format (pre-coated/uncoated, strips vs full plate), and bulk or custom packaging. Click Talk to a Scientist to submit a request form, email us at support@biohippo.com, or explore our Research Services for additional support. Our team will be in contact with you shortly.
Lu Chen et al. (2021) Programmable immune activating electrospun fibers for skin regeneration. Bioactive Materials. 10.1016/j.bioactmat.2021.02.022
Chuan Wang et al. (2023) Circulating Tfr/Tfh cell imbalance may contribute to the immunopathogenesis of pemphigus. Journal of the European Academy of Dermatology and Venereology. 10.1111/jdv.19338
Yanjuan Liu et al. (2021) The TGF-β/miR-31/CEACAM1-S axis inhibits CD4+CD25+ Treg differentiation in systemic lupus erythematosus. Immunology and Cell Biology. 10.1111/imcb.12449
Dan Lv et al. (2024) Microbial imidazole propionate affects glomerular filtration rate in patients with diabetic nephropathy through association with HSP90α. Biochimica et Biophysica ACTA-Molecular Cell Research. 10.1016/j.bbamcr.2024.119703
Das Dayasagar et al. (2023) Dominance and improved survivability of human γδT17 cell subset aggravates the immunopathogenesis of pemphigus vulgaris. Immunologic Research. 10.1007/s12026-023-09413-0
Limin Bai et al. (2023) Impact of a Novel Hydrogel with Injectable Platelet-Rich Fibrin in Diabetic Wound Healing. Journal of Diabetes Research. 10.1155/2023/7532637
Kai Tian et al. (2023) Autologous i-PRF promotes healing of radiation-induced skin injury. Wound Repair and Regeneration. 10.1111/wrr.13083
Hu Feng et al. (2018) Article Effect and Mechanism of Ganoderma lucidum Polysaccharides on Human Fibroblasts and Skin Wound Healing in Mice. Chinese Journal of Integrative Medicine. 10.1007/s11655-018-3060-9
