| Field | Specification |
|---|---|
| Target | |
| Alternative names | Lipopolysaccharide-binding protein;LBP;LBP; |
| Gene ID | |
| UniProt # | |
| Reactivity | |
| Applications | |
| Immunogen | Expression system for standard: NS0; Immunogen sequence: A26-V481 |
| Expression system | |
| Expression region | |
| Assay type | |
| Sample type(s) | Cell Culture Supernatant, Cell Lysate, Serum, Plasma |
| Sensitivity | |
| Detection range | |
| Assay time | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Assay Principle
This sandwich ELISA quantifies human lipopolysaccharide-binding protein (gene LBP) in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Strips are pre-coated with a capture antibody. After the sample is added, a biotinylated detection antibody binds the captured protein, and an avidin–biotin–peroxidase complex supplies the enzyme. TMB develops color in proportion to the bound target; the reaction is stopped and read at 450 nm, and concentrations are read from the standard curve.
Lipopolysaccharide-binding protein (LBP) is an acute-phase plasma protein made mainly by the liver. It binds the lipid A part of bacterial lipopolysaccharide and hands it to CD14, which then presents it to the TLR4/MD-2 receptor, greatly increasing cell sensitivity to Gram-negative bacteria. LBP is studied in innate immunity, sepsis and inflammation research.
Kit Components
| Anti-Human LBP Pre-coated 96-well strip microplate | 1 plate (96 wells) |
|---|---|
| Human LBP Standard | 2 vials, 50 ng/tube |
| Human LBP Biotinylated antibody (100x) | 100 µL |
| Avidin-Biotin-Peroxidase Complex (100x) | 100 µL |
| Sample Diluent | 30 mL |
| Antibody Diluent | 12 mL |
| Avidin-Biotin-Peroxidase Diluent | 12 mL |
| Color Developing Reagent (TMB) | 10 mL |
| Stop Solution | 10 mL |
| Wash Buffer (25x) | 20 mL |
| Adhesive plate sealers | 4 |
Also required (not supplied): microplate reader for 450 nm; incubator; automated plate washer (optional); precision pipettes for 0.5 µL through 1 mL volumes of aqueous solutions; multichannel pipettes (recommended for many samples); deionized or distilled water; 500 mL graduated cylinders; test tubes for dilution.
Kit components are not sold separately.
Performance Data
| Species | Human |
|---|---|
| Sample types | Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) |
| Assay range | 0.78 ng/mL–50 ng/mL |
| Sensitivity | <50 pg/mL |
| Standard | Recombinant human LBP (Ala26–Val481), expressed in NS0 |
| Cross-reactivity | None detected against related proteins in supplier testing |
| Intra-assay CV | 4.5–6.4% (3 samples, n = 16 each) |
| Inter-assay CV | 5.6–6.7% (3 samples, n = 24 each) |
| Format | 96 wells, removable strips |
Sample dilution: start at 1:1000. In the supplier’s tests, serum and plasma samples measured 1000–6000 ng/mL.
Example standard curve (OD450, ng/mL): 0 = 0.021; 0.78 = 0.078; 1.56 = 0.124; 3.12 = 0.227; 6.25 = 0.398; 12.5 = 0.712; 25 = 1.242; 50 = 1.802. Run a new standard curve on every plate.
Storage & Handling
Store at 4 °C for up to 6 months or −20 °C for up to 12 months. Avoid repeated freeze–thaw cycles. Ships on gel ice and tolerates up to 3 days at room temperature; freeze on arrival.
Safety
Sample data
| Concentration (ng/ml) | 0 | 0.78 | 1.56 | 3.12 | 6.25 | 12.5 | 25 | 50 |
| O.D. | 0.021 | 0.078 | 0.124 | 0.227 | 0.398 | 0.712 | 1.242 | 1.802 |
Intra/inter assay consistency
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 1485 | 4533 | 23562 | 1518 | 4575 | 21374 |
| Standard deviation | 66.82 | 271.98 | 1507.96 | 85 | 297.37 | 1432.05 |
| CV (%) | 4.5% | 6% | 6.4% | 5.6% | 6.5% | 6.7% |
Kit components
Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4Materials required but not provided
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Mónica Manzano et al. (2022) Relationship of Diet to Gut Microbiota and Inflammatory Biomarkers in People with HIV. Nutrients. 10.3390/nu14061221
Zhang Li-Ping et al. (2023) Pyroptotic MAITs link microbial translocation with severity of alcohol-associated liver disease. Hepatology Communications. 10.1097/HC9.0000000000000159
Liu Yun et al. (2024) SERPINA3, FGA, AGP1, ITIH3 and SAA1 as novel biomarkers for eosinophilic granulomatosis with polyangiitis diagnosis and activity assessment. Rheumatology. 10.1093/rheumatology/keae187
Xiuxia Yuan et al. (2022) Gut mycobiota dysbiosis in drug-naïve, first-episode schizophrenia. Schizophrenia Research. 10.1016/j.schres.2022.10.011
