| Field | Specification |
|---|---|
| Target | |
| Alternative names | FLAP-1; FLIIAP1; GCF-2; GCF2; HUFI-1; MGC10947; MGC119738; MGC119739; TRIP; GC-binding factor 2|NEDD8-conjugating enzyme|transcription factor 9-like |
| UniProt # | |
| Species | |
| Applications | |
| Assay type | |
| Sensitivity | |
| Detection range | |
| Catalog no. (Mfr.) | |
| Main SKU |
Scientific Background
The GCF2 cDNA contains an internal region with 98% nucleotide identity with the first 309 bp of the GCF cDNA, but the remainder of the 2 sequences share no significant homology. The predicted 752-amino acid GCF2 protein has a pI of 4.4. The authors confirmed the calculated molecular mass of 83 kD by mass spectroscopy. However, GCF2 migrates as a 160-kD protein by SDS-PAGE. the discrepancy between the calculated and observed masses is due either to the acidic nature of the protein or to an unusual ability of GCF2 to form very stable dimers. GCF2 binds to EGFR promoter fragments in vitro. Cotransfection assays showed that GCF2 represses transcription from 3 different promoters, including that of EGFR.
Assay Principle
This assay employs a two-site sandwich ELISA to quantitate LRRFIP1 in samples. An antibody specific for LRRFIP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLRRFIP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LRRFIP1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LRRFIP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Performance Data
| Assay Type | Sandwich ELISA |
|---|---|
| Detection Range | 0.312-20 ng/mL |
| Sensitivity | 0.127 ng/mL |
| Species Reactivity | Human (Homo sapiens) |
| Stability | The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C). |
| Manufacturer No. | AE34990HU |
| UniProt # | Q32MZ4 |
| Alternative Names | FLAP-1; FLIIAP1; GCF-2; GCF2; HUFI-1; MGC10947; MGC119738; MGC119739; TRIP; GC-binding factor 2|NEDD8-conjugating enzyme|transcription factor 9-like |
Assay Workflow
The kit includes the components required for standard-curve quantification (see the kit manual for the component table and validated sample types). Absorbance is read on a standard microplate reader and concentrations are interpolated from the standard curve.
Safety & Handling
For research use only (RUO). Not for use in diagnostic or therapeutic procedures. Handle all samples and reagents using standard laboratory safety practices.
Related Products
Explore additional Human ELISA kits from Wuhan Abebio Science Co Ltd, or contact us for related assays for LRRFIP1 research.
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