| Field | Specification |
|---|---|
| Target | |
| Alternative names | Macrophage colony-stimulating factor 1;CSF-1;M-CSF;MCSF;Lanimostim;Processed macrophage colony-stimulating factor 1;CSF1; |
| Gene ID | |
| UniProt # | |
| Reactivity | |
| Applications | |
| Immunogen | Expression system for standard: E.coli; Immunogen sequence: E33-S190 |
| Expression system | |
| Expression region | |
| Assay type | |
| Sample type(s) | Cell Culture Supernatant, Serum, Plasma, Saliva, Urine |
| Sensitivity | |
| Detection range | |
| Assay time | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Assay Principle
This sandwich ELISA quantifies human macrophage colony-stimulating factor 1 (gene CSF1) in cell culture supernatants, serum, plasma (heparin, EDTA), saliva and urine. Strips are pre-coated with a capture antibody. After the sample is added, a biotinylated detection antibody binds the captured protein, and an avidin–biotin–peroxidase complex supplies the enzyme. TMB develops color in proportion to the bound target; the reaction is stopped and read at 450 nm, and concentrations are read from the standard curve.
M-CSF (CSF1) is a cytokine that supports the survival, proliferation and differentiation of monocytes, macrophages and their precursors by signaling through the CSF1R receptor. It is also needed for osteoclast formation and bone resorption, and influences inflammation, fertility and cell migration, so it is measured in studies of macrophage biology, bone remodeling and inflammatory disease.
Kit Components
| Anti-Human CSF1 Pre-coated 96-well strip microplate | 1 plate (96 wells) |
|---|---|
| Human CSF1 Standard | 2 vials, 10 ng/tube |
| Human CSF1 Biotinylated antibody (100x) | 100 µL |
| Avidin-Biotin-Peroxidase Complex (100x) | 100 µL |
| Sample Diluent | 30 mL |
| Antibody Diluent | 12 mL |
| Avidin-Biotin-Peroxidase Diluent | 12 mL |
| Color Developing Reagent (TMB) | 10 mL |
| Stop Solution | 10 mL |
| Wash Buffer (25x) | 20 mL |
| Adhesive plate sealers | 4 |
Also required (not supplied): microplate reader for 450 nm; incubator; automated plate washer (optional); precision pipettes for 0.5 µL through 1 mL volumes of aqueous solutions; multichannel pipettes (recommended for many samples); deionized or distilled water; 500 mL graduated cylinders; test tubes for dilution.
Kit components are not sold separately.
Performance Data
| Species | Human |
|---|---|
| Sample types | Cell culture supernatants, serum, plasma (heparin, EDTA), saliva and urine |
| Assay range | 62.5 pg/mL–4,000 pg/mL |
| Sensitivity | <10 pg/mL |
| Standard | Recombinant human CSF1 (Glu33–Ser190), expressed in E. coli |
| Cross-reactivity | None detected against related proteins in supplier testing |
| Intra-assay CV | 4.6–5.3% (3 samples, n = 16 each) |
| Inter-assay CV | 4.6–6.5% (3 samples, n = 24 each) |
| Format | 96 wells, removable strips |
Sample dilution: start at 1:1. In the supplier’s tests, serum and plasma samples measured below the lowest standard.
Example standard curve (OD450, pg/mL): 0 = 0.008; 62.5 = 0.078; 125 = 0.116; 250 = 0.225; 500 = 0.407; 1000 = 0.753; 2000 = 1.292; 4000 = 1.91. Run a new standard curve on every plate.
Storage & Handling
Store at 4 °C for up to 6 months or −20 °C for up to 12 months. Avoid repeated freeze–thaw cycles. Ships on gel ice and tolerates up to 3 days at room temperature; freeze on arrival.
Safety
Sample data
| Concentration (pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | 4000 |
| O.D. | 0.008 | 0.116 | 0.225 | 0.407 | 0.753 | 1.292 | 1.91 | 2.44 |
Intra/inter assay consistency
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 152 | 681 | 1139 | 140 | 716 | 1136 |
| Standard deviation | 8.05 | 32.68 | 52.39 | 9.1 | 40.09 | 52.25 |
| CV (%) | 5.3% | 4.8% | 4.6% | 6.5% | 5.6% | 4.6% |
Kit components
Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4Materials required but not provided
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Heng Zhang et al. (2026) The CSF1+ tumor cell–SPP1+ macrophage axis drives gastric cancer progression and immunotherapy resistance. Frontiers in Immunology. 10.3389/fimmu.2026.1817573
Zhu Wei et al. (2019) Loss of the androgen receptor suppresses intrarenal calcium oxalate crystals deposition via altering macrophage recruitment/M2 polarization with change of the miR-185-5p/CSF-1 signals. Cell Death & Disease. 10.1038/s41419-019-1358-y
Lien Huang-Chun et al. (2025) Carcinomas exhibiting epithelial–mesenchymal transition manifest an M2 macrophage-enriched tumor immune microenvironment. Breast Cancer Research. 10.1186/s13058-025-02119-1
Calabriso Nadia et al. (2015) Multiple anti-inflammatory and anti-atherosclerotic properties of red wine polyphenolic extracts: differential role of hydroxycinnamic acids, flavonols and stilbenes on endothelial inflammatory gene expression. European Journal of Nutrition. 10.1007/s00394-015-0865-6
