| Field | Specification |
|---|---|
| Mfr No | |
| Assay Type | |
| Detection Range | |
| Product Type | |
| Sample Type(s) | cell culture supernatant, serum, plasma, cell lysate |
| Sensitivity | |
| Species | |
| Storage | |
| Target |
Assay Principle
Human Menin ELISA is a solid phase ELISA designed to measure human Menin levels in cell culture supernatants, serum, plasma and cell lysates. The detection range is from 0.18 to 150 ng/mL. The levels of human Menin samples are parallel to the standard curves obtained using the kit standards linearly. These results indicate that this kit can be used to determine relative mass values for human Menin protein.
Typical Data This standard curve(R 2 =1.000) is provided in Fig. 3 for demonstration only. A standard curve should be generated for each set of samples assayed.
Sensitivity The minimum detectable dose (MOD) of human Menin is typically 100 pg/ml.
Human Menin ELISA Kit is a Solid-phase sandwich ELISA for the quantitative determination of Menin. The microplate is pre-coated with a capture antibody specific to the target. Target present in the sample or standard binds the immobilized antibody, is detected with a biotinylated detection antibody and streptavidin-HRP, and quantified colorimetrically against a standard curve.
Kit Components
The kit provides a pre-coated microplate, target standard for standard-curve generation, detection reagents (detection antibody and enzyme conjugate), wash buffer, substrate, and stop solution. Refer to the product datasheet for the complete component list and quantities.
Performance Data
| Target | Menin |
|---|---|
| Species | Human |
| Sample Type | cell culture supernatant, serum, plasma, cell lysate |
| Assay Type | Sandwich |
| Test Principle | Solid-phase sandwich ELISA |
| Sensitivity | 100 pg/mL |
| Detection Range | 0.18–150 ng/mL |
| Standard | Recombinant Human Menin |
| Specificity | This assay recognizes natural and recombinant human Menin. |
| Storage | Store the unopened kit at 2-8°C. |
✓ Quality tested for reproducibility and lot-to-lot consistency
Safety & Handling
This kit is validated for cell culture supernatant, serum, plasma, cell lysate. Other matrices may require additional in-lab validation.
This assay has a sensitivity of 100 pg/mL and a detection range of 0.18–150 ng/mL. Samples reading outside the range should be diluted or concentrated.
The kit provides a pre-coated microplate, target standard for standard-curve generation, detection reagents (detection antibody and enzyme conjugate), wash buffer, substrate, and stop solution. See the datasheet for the full component list and quantities.
A standard microplate reader capable of measuring absorbance at 450 nm is required for this sandwich ELISA.
Customization & Add-ons: Need this ELISA kit tailored to your workflow? We can help with bulk or custom pack sizes, species or analyte variants, matched antibody pairs and standards, alternate plate formats (48- or 96-well, strip-well), and assay development or custom ELISA solutions for targets not in our catalog. Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services — our team will follow up with feasibility details and next steps.
