| Field | Specification |
|---|---|
| Target | |
| Alternative names | Lactadherin;Breast epithelial antigen BA46;HMFG;MFGM;Milk fat globule-EGF factor 8;MFG-E8;SED1;Lactadherin short form;Medin;MFGE8; |
| Gene ID | |
| UniProt # | |
| Reactivity | |
| Applications | |
| Immunogen | Expression system for standard: NS0; Immunogen sequence: L24-C387 |
| Expression system | |
| Expression region | |
| Assay type | |
| Sample type(s) | Cell Culture Supernatant, Serum, Plasma, Saliva, Urine, Milk |
| Sensitivity | |
| Detection range | |
| Assay time | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Assay Principle
This sandwich ELISA quantifies human lactadherin (gene MFGE8) in cell culture supernatants, serum, plasma (heparin, EDTA), saliva, urine and human milk. Strips are pre-coated with a capture antibody. After the sample is added, a biotinylated detection antibody binds the captured protein, and an avidin–biotin–peroxidase complex supplies the enzyme. TMB develops color in proportion to the bound target; the reaction is stopped and read at 450 nm, and concentrations are read from the standard curve.
Lactadherin (MFGE8, also called milk fat globule-EGF factor 8) is a secreted glycoprotein that bridges apoptotic cells to phagocytes. Its discoidin-like domains bind phosphatidylserine on dying cells, while its EGF-like domain binds αvβ3 and αvβ5 integrins on macrophages, promoting efferocytosis. MFGE8 is studied in inflammation resolution, autoimmunity, tissue repair and milk fat globules.
Kit Components
| Anti-Human MFGE8 Pre-coated 96-well strip microplate | 1 plate (96 wells) |
|---|---|
| Human MFGE8 Standard | 2 vials, 10 ng/tube |
| Human MFGE8 Biotinylated antibody (100x) | 100 µL |
| Avidin-Biotin-Peroxidase Complex (100x) | 100 µL |
| Sample Diluent | 30 mL |
| Antibody Diluent | 12 mL |
| Avidin-Biotin-Peroxidase Diluent | 12 mL |
| Color Developing Reagent (TMB) | 10 mL |
| Stop Solution | 10 mL |
| Wash Buffer (25x) | 20 mL |
| Adhesive plate sealers | 4 |
Also required (not supplied): microplate reader for 450 nm; incubator; automated plate washer (optional); precision pipettes for 0.5 µL through 1 mL volumes of aqueous solutions; multichannel pipettes (recommended for many samples); deionized or distilled water; 500 mL graduated cylinders; test tubes for dilution.
Kit components are not sold separately.
Performance Data
| Species | Human |
|---|---|
| Sample types | Cell culture supernatants, serum, plasma (heparin, EDTA), saliva, urine and human milk |
| Assay range | 62.5 pg/mL–4,000 pg/mL |
| Sensitivity | <10 pg/mL |
| Standard | Recombinant human MFGE8 (Leu24–Cys387), expressed in NS0 |
| Cross-reactivity | None detected against related proteins in supplier testing |
| Intra-assay CV | 5.6–7.6% (3 samples, n = 16 each) |
| Inter-assay CV | 5.8–8.9% (3 samples, n = 24 each) |
| Format | 96 wells, removable strips |
Sample dilution: start at 1:1. In the supplier’s tests, serum samples measured about 800 pg/mL.
Example standard curve (OD450, pg/mL): 0 = 0.006; 62.5 = 0.06; 125 = 0.129; 250 = 0.256; 500 = 0.442; 1000 = 0.829; 2000 = 1.384; 4000 = 1.979. Run a new standard curve on every plate.
Storage & Handling
Store at 4 °C for up to 6 months or −20 °C for up to 12 months. Avoid repeated freeze–thaw cycles. Ships on gel ice and tolerates up to 3 days at room temperature; freeze on arrival.
Safety
Sample data
| Concentration (pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | 4000 |
| O.D. | 0.006 | 0.06 | 0.129 | 0.256 | 0.442 | 0.829 | 1.384 | 1.979 |
Intra/inter assay consistency
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 100 | 660 | 1196 | 101 | 717 | 1149 |
| Standard deviation | 5.8 | 36.96 | 90.89 | 8.28 | 41.58 | 102.26 |
| CV (%) | 5.8% | 5.6% | 7.6% | 8.2% | 5.8% | 8.9% |
Kit components
Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4Materials required but not provided
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Jinwu Bai et al. (2023) MFG-E8 promotes osteogenic differentiation of human bone marrow mesenchymal stem cells through GSK3β/β-catenin signaling pathway. FASEB Journal. 10.1096/fj.202201417RRR
Qiongfei Pei et al. (2025) MFG-E8 in Kawasaki Disease: Role in Endothelial Injury and Diagnostic Potential. Journal of Inflammation Research. 10.2147/JIR.S547634
Sapkota Bishwa R. et al. (2020) A rare missense variant in the milk fat globule-EGF factor 8 (MFGE8) increases T2DM susceptibility and cardiovascular disease risk with population-specific effects. ACTA Diabetologica. 10.1007/s00592-019-01463-x
Wang Jing-yu et al. (2022) Goal-directed therapy based on rScO2 monitoring in elderly patients with one-lung ventilation: a randomized trial on perioperative inflammation and postoperative delirium. Trials. 10.1186/s13063-022-06654-6
Madhusmita Rout et al. (2024) Contribution of circulating Mfge8 to human T2DM and cardiovascular disease. Gene. 10.1016/j.gene.2024.148712
Becker-Dreps Sylvia et al. (2017) Innate Immune Factors in Mothers' Breast Milk and Their Lack of Association With Rotavirus Vaccine Immunogenicity in Nicaraguan Infants. Journal of the Pediatric Infectious Diseases Society. 10.1093/jpids/piv076
