| Field | Specification |
|---|---|
| Target | |
| Alternative names | Matrilysin;3.4.24.23;Matrin;Matrix metalloproteinase-7;MMP-7;Pump-1 protease;Uterine metalloproteinase;MMP7;MPSL1, PUMP1; |
| Gene ID | |
| UniProt # | |
| Reactivity | |
| Applications | |
| Immunogen | Expression system for standard: NS0; Immunogen sequence: L18-K267 |
| Expression system | |
| Expression region | |
| Assay type | |
| Sample type(s) | Cell Culture Supernatant, Serum, Plasma, Saliva, Urine |
| Sensitivity | |
| Detection range | |
| Assay time | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Assay Principle
This sandwich ELISA quantifies human matrilysin (gene MMP7) in cell culture supernatants, serum, plasma (heparin), saliva and urine. Strips are pre-coated with a capture antibody. After the sample is added, a biotinylated detection antibody binds the captured protein, and an avidin–biotin–peroxidase complex supplies the enzyme. TMB develops color in proportion to the bound target; the reaction is stopped and read at 450 nm, and concentrations are read from the standard curve.
Matrilysin (MMP-7) is the smallest matrix metalloproteinase, lacking the hemopexin domain found in most family members. It degrades casein, gelatins and fibronectin and can activate other MMPs, and it is produced by many epithelial cells and studied in mucosal defense and tumor biology.
Kit Components
| Anti-Human MMP7 Pre-coated 96-well strip microplate | 1 plate (96 wells) |
|---|---|
| Human MMP7 Standard | 2 vials, 10 ng/tube |
| Human MMP7 Biotinylated antibody (100x) | 100 µL |
| Avidin-Biotin-Peroxidase Complex (100x) | 100 µL |
| Sample Diluent | 30 mL |
| Antibody Diluent | 12 mL |
| Avidin-Biotin-Peroxidase Diluent | 12 mL |
| Color Developing Reagent (TMB) | 10 mL |
| Stop Solution | 10 mL |
| Wash Buffer (25x) | 20 mL |
| Adhesive plate sealers | 4 |
Also required (not supplied): microplate reader for 450 nm; incubator; automated plate washer (optional); precision pipettes for 0.5 µL through 1 mL volumes of aqueous solutions; multichannel pipettes (recommended for many samples); deionized or distilled water; 500 mL graduated cylinders; test tubes for dilution.
Kit components are not sold separately.
Performance Data
| Species | Human |
|---|---|
| Sample types | Cell culture supernatants, serum, plasma (heparin), saliva and urine |
| Assay range | 156 pg/mL–10,000 pg/mL |
| Sensitivity | <6 pg/mL |
| Standard | Recombinant human MMP7 (Leu18–Lys267), expressed in NS0 |
| Cross-reactivity | None detected against related proteins in supplier testing |
| Intra-assay CV | 4.2–6.6% (3 samples, n = 16 each) |
| Inter-assay CV | 4.7–8.2% (3 samples, n = 24 each) |
| Format | 96 wells, removable strips |
Sample dilution: start at 1:1. In the supplier’s tests, serum and plasma samples measured 0.85–5.28 ng/mL.
Example standard curve (OD450, pg/mL): 0 = 0.01; 156 = 0.072; 312 = 0.176; 625 = 0.348; 1250 = 0.576; 2500 = 1.135; 5000 = 1.829; 10000 = 2.326. Run a new standard curve on every plate.
Storage & Handling
Store at 4 °C for up to 6 months or −20 °C for up to 12 months. Avoid repeated freeze–thaw cycles. Ships on gel ice and tolerates up to 3 days at room temperature; freeze on arrival.
Safety
Sample data
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.01 | 0.072 | 0.176 | 0.348 | 0.576 | 1.135 | 1.829 | 2.326 |
Intra/inter assay consistency
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 272 | 1042 | 5090 | 256 | 1008 | 5032 |
| Standard deviation | 12.51 | 43.76 | 336.64 | 15.36 | 47.37 | 412.62 |
| CV (%) | 4.6% | 4.2% | 6.6% | 6% | 4.7% | 8.2% |
Kit components
Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4Materials required but not provided
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Yu Sun et al. (2023) NanoVelcro-captured CTC number concomitant with enhanced serum levels of MMP7 and MMP9 enables accurate prediction of metastasis and poor prognosis in patients with lung adenocarcinoma. International Journal of Nanomedicine. 10.2147/IJN.S144033
Jiang Jingying et al. (2024) Serum matrix metalloproteinase-7 for discriminating biliary atresia: a diagnostic accuracy and validation study. Journal of Translational Medicine. 10.1186/s12967-024-05442-x
Hao-yu Lin et al. (2017) CCR10 activation stimulates the invasion and migration of breast cancer cells through the ERK1/2/MMP-7 signaling pathway. International Immunopharmacology. 10.1016/j.intimp.2017.07.018
Kim Kyoung Soo et al. (2011) Implication of MMP-9 and urokinase plasminogen activator (uPA) in the activation of pro-matrix metalloproteinase (MMP)-13. Rheumatology International. 10.1007/s00296-011-2095-4
Hyun M. CHOI et al. (2011) Increased levels of thymosin β4 in synovial fluid of patients with rheumatoid arthritis: association of thymosin β4 with other factors that are involved in inflammation and bone erosion in joints. International Journal of Rheumatic Diseases. 10.1111/j.1756-185X.2011.01652.x
