| Field | Specification |
|---|---|
| Organism | |
| Applications | |
| Tissue | |
| Disease | Left hard palate (local recurrence = 3A cell line) and lymph node metastasis (=3B cell line) |
| Age | |
| Sex | |
| Ethnicity | |
| Growth properties | |
| Biosafety level | |
| Product format | Frozen |
| Storage | |
| Shipping | |
| Catalog no. (Mfr.) | |
| Main SKU |
Overview
The UM-HMC series, encompassing UM-HMC-1, UM-HMC-3B, and UM-HMC-3A, are pioneering cell lines derived from human salivary mucoepidermoid carcinomas, pivotal for advancing research in oncology and cellular biology. Originating from a primary tumor (UM-HMC-1) from a male patient, and recurrence and lymph node metastasis from a female patient (UM-HMC-3A and UM-HMC-3B, respectively), these cell lines have been instrumental in characterizing the tumorigenic potential and cancer stem cell properties within salivary gland carcinomas.
Key elements and design rationale
- Model identity: Human Mucoepidermoid Carcinoma Cell Line (UM-HMC-3A) is supplied as a tumor cell line derived from Human salivary gland.
- Growth properties: Adherent, epithelial
- Growth conditions: PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. PriGrow III (TM003) + 10% FBS(Regular*) + 1% L-glutamine (G275) + 400 ng/ml hydrocortisone (TM066) + 20 ng/ml rhEGF (Z100139) + 5 ug/ml recombinant human Insulin (Z101065) + 1% Penicillin/Streptomycin Solution (G255), 37.0°C, 5% CO₂.Note: re-suspend cells well at each stage (thawing, post-centrifugation etc.) as cells have a tendency to clump. Freeze-thaw viability for this cell line varies. For best results, seed thawed cells, collect floating cells the next day, centrifuge and re-plate into a new flask. *Do not heat-inactivate
- Product format: Frozen, BSL-2
This cell-based model is generally used in neurobiology, differentiation, and cell signaling studies. Donor/background information is available for contextual interpretation.
Biological background
Studies utilizing these cell lines have shed light on critical aspects of cancer biology, including the role of ALDH/CD44 in identifying tumorigenic cancer stem cells, the necessity of mTOR signaling for the survival of these cancer stem cells, and the influence of p53 in regulating Bmi-1-driven self-renewal and stemness. As such, the UM-HMC series represents a valuable resource for researchers aiming to explore the molecular mechanisms underlying salivary gland cancer progression, metastasis, and treatment resistance, thereby offering potential pathways for developing targeted therapies.Complete list of cell lines from this panel: Cat. T8160 - Human Mucoepidermoid Carcinoma Cell Line (UM-HMC-3A)Cat. T8161 - Human Mucoepidermoid Carcinoma Cell Line (UM-HMC-3B) Cat. T8173 - Human Mucoepidermoid Carcinoma Cell Line (UM-HMC-1) Donor/background information provided for this product: Female, 73, Caucasian, , Left hard palate (local recurrence = 3A cell line) and lymph node metastasis (=3B cell line), T4BN, stage IVb, intermediate grade, + for both perineural and angio-lymphatic invasion..
Research relevance and current trends
- Cell-line models continue to be used for tumor phenotype comparison, pathway perturbation studies, and assay development in controlled in vitro systems.
- Engineered and subtype-defined tumor lines are often used to compare growth behavior, reporter output, and response patterns across matched experimental conditions.
- When metastatic or lineage features are described, investigators commonly interpret results alongside morphology, passage history, and culture environment.
Common research applications
- Neurobiology-focused studies of growth state, differentiation-associated morphology, and cell signaling changes in culture.
- Cell-based assays that compare experimental perturbations across defined media and substrate conditions.
- Phenotype tracking using morphology, marker expression, or reporter output where applicable.
Changes in morphology, growth rate, viability, or reporter signal are typically interpreted together with passage history, culture matrix, and the specified growth conditions for the model.
Notes for experimental interpretation
- Morphology, doubling behavior, and reporter or marker output can shift with passage number, substrate choice, and medium composition; these variables should be recorded alongside experimental readouts.
- Matched controls such as parental cells, untreated cultures, or parallel cultures maintained under identical conditions help distinguish background effects from biology of interest.
Culture and product details
- Growth Conditions: PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. PriGrow III (TM003) + 10% FBS(Regular*) + 1% L-glutamine (G275) + 400 ng/ml hydrocortisone (TM066) + 20 ng/ml rhEGF (Z100139) + 5 ug/ml recombinant human Insulin (Z101065) + 1% Penicillin/Streptomycin Solution (G255), 37.0°C, 5% CO₂.Note: re-suspend cells well at each stage (thawing, post-centrifugation etc.) as cells have a tendency to clump. Freeze-thaw viability for this cell line varies. For best results, seed thawed cells, collect floating cells the next day, centrifuge and re-plate into a new flask. *Do not heat-inactivate
- Split Ratio: 1:10 to 1:4
- Thaw cells quickly in a 37°C water bath while agitating gently (maximum 2 minutes). The vial cap should be kept above the water level to minimize the risk of contamination.
- Decontaminate the vial by spraying and wiping the exterior of the vial with 70% ethanol. From this point onwards, all operations should be strictly carried out inside a biological safety cabinet using aseptic conditions.
- Transfer the cell suspension into a 15ml sterile conical tube containing 5ml of pre-warmed, complete growth media. Pipette up and down several times to beak up clumps. Centrifuge cells at 800 RPM for 5-7 minutes.
- Aspirate the supernatant without disturbing the cell pellet. Re-suspend the cell pellet in the recommended pre-warmed, complete growth media and dispense into a T25 culture flask.
- Incubate the cells at the recommended conditions.
- Aspirate the culture media, gently wash adherent layer with 1X PBS 2-3 times, and add 2-3ml of pre-warmed 0.25% Trypsin-EDTA to the culture vessel.
- Observe the cells under a microscope to confirm detachment (typically within 5-10 minutes). Cells that are difficult to detach can be put in 37°C, for several minutes to facilitate detachment.
- Neutralize Trypsin-EDTA by adding an equal volume of the complete growth media into the culture vessel.
- Transfer the culture suspension into a sterile centrifuge tube, and centrifuge at 800 RPM for 5 minutes. The actual centrifuge duration and speed may vary depending on the cell type.
- Aspirate the supernatant, and re-suspend the pellet with pre-warmed fresh complete growth media. Add appropriate aliquots of the cell suspension to new culture vessels, as desired.
- Incubate the cells at the recommended conditions.
How should I handle live cells once I receive them?
https://www.abmgood.com/immortalized-cells-documents.html
Following these guidelines will help ensure optimal cell viability and performance.
Why are these cells classified as biosafety level II?
What is your warranty or return policy?
Please refer to the following link for full information:
https://www.abmgood.com/terms
For additional questions, our Order team is happy to assist and can be reached at order@abmgood.com.
How many times can cells divide?
Primary cells have a limited lifespan and will undergo a finite number of population doublings before entering senescence. The exact number varies by cell type and culture conditions.
Immortalized cell lines are capable of extended or indefinite proliferation under proper culture conditions, although growth characteristics may vary between lines.
Do I need Applied Cell Extracellular Matrix (G422) if I am using PriCoat™ flasks?
Cell line sourcing and selection (species, tissue, and disease model matching) · Stable cell line engineering (overexpression, knockdown, knockout via CRISPR/Cas9, shRNA, sgRNA) · Reporter gene integration (GFP, RFP, luciferase, fluorescent/bioluminescent constructs) · Genome editing and knockin (point mutations, tagged endogenous proteins, conditional alleles) · Inducible expression systems (Tet-On/Off and regulatable constructs) · Drug resistance marker selection (puromycin, G418, hygromycin, and others) · Custom growth and media optimisation for specific assay requirements · Scale-up production for high-throughput screening campaigns · Authentication and QC services (STR profiling, mycoplasma testing, viability assessment). Talk to a Scientist or contact support@biohippo.com.
