| Field | Specification |
|---|---|
| Mfr No | |
| Detection Range | |
| Product Type | |
| Sample Type(s) | serum, plasma |
| Sensitivity | |
| Species | |
| Storage | |
| Target |
Assay Principle
Tribioscience’s Fast Human Neurofilament-Light Chain (NF-L) ELISA is designed to quantitatively detect human NF-L levels in serum, plasma, and other biological samples. The main feature is that the kit uses our novel proprietary approaches to combine samples and detections into a one-step instead of the complicated traditional methods. It makes the assay simple, easy, accurate, and fast. The Hands-on time can be within 2 hours, with no need for 4-5 hours (Fig. 1). The detection range is from 0.156 to 10 ng/mL. The levels of human NF-L samples are parallel to the standard curves obtained using the kit standards linearly. Therefore, the kit can be used to determine relative mass values for natural human NF-L protein.
Typical Data This standard curve (R 2 =1.000) is provided for demonstration only. A standard curve should be generated for each set of samples assayed. Fig. 3 is an example of typical Data.
Sensitivity The minimum detectable dose (MOD) of human is typically 100 pg/ml. The Intra-assay CV and the Inter-assay CV are ˂10%.
Human Neurofilament-Light Chain (NF-L) ELISA Kit is a Solid-phase ELISA for the quantitative determination of Neurofilament-Light Chain (NF-L). The microplate is pre-coated with a capture antibody specific to the target. Target present in the sample or standard binds the immobilized antibody, is detected with a biotinylated detection antibody and streptavidin-HRP, and quantified colorimetrically against a standard curve.
Kit Components
The kit provides a pre-coated microplate, target standard for standard-curve generation, detection reagents (detection antibody and enzyme conjugate), wash buffer, substrate, and stop solution. Refer to the product datasheet for the complete component list and quantities.
Performance Data
| Target | Neurofilament-Light Chain (NF-L) |
|---|---|
| Species | Human |
| Sample Type | serum, plasma |
| Test Principle | Solid-phase ELISA |
| Sensitivity | 100 pg/mL |
| Detection Range | 0.156–10 ng/mL |
| Precision | Intra-assay CV 10% |
| Standard | Recombinant Human Neurofilament-Light Chain (NF-L) |
| Specificity | This assay recognizes natural and recombinant human NF-L. |
| Storage | Store the unopened kit at 2-8°C. | Components: Return unused wells to the foil pouch. Reseal along the entire edge of the zip-seal. May be stored for up to 1 month at 2-8°C.; Aliquot and store at -20°C for up to 1 month in a manual defrost freezer. Avoid repeated freeze-thaw cycles.; May be stored for up to 3 months at 2-8°C. |
✓ Quality tested for reproducibility and lot-to-lot consistency
Safety & Handling
This kit is validated for serum, plasma. Other matrices may require additional in-lab validation.
This assay has a sensitivity of 100 pg/mL and a detection range of 0.156–10 ng/mL. Samples reading outside the range should be diluted or concentrated.
The kit provides a pre-coated microplate, target standard for standard-curve generation, detection reagents (detection antibody and enzyme conjugate), wash buffer, substrate, and stop solution. See the datasheet for the full component list and quantities.
A standard microplate reader capable of measuring absorbance at 450 nm is required for this sandwich ELISA.
Customization & Add-ons: Need this ELISA kit tailored to your workflow? We can help with bulk or custom pack sizes, species or analyte variants, matched antibody pairs and standards, alternate plate formats (48- or 96-well, strip-well), and assay development or custom ELISA solutions for targets not in our catalog. Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services — our team will follow up with feasibility details and next steps.
