| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | CMT1F; CMT2E; NF-L; NF68; NFL; 68 kDa neurofilament protein; Neurofilament triplet L protein |
| Detection Range | |
| Gene ID | |
| Product Type | |
| Sample Type(s) | serum, plasma, cerebrospinal fluid or other biological fluids. |
| Sensitivity | |
| Species | |
| UniProt # |
Assay Principle
Neurofilament, Light Polypeptide (NEFL) is a biomarker protein expressed in human tissues and biological fluids. It is also referred to as CMT1F; CMT2E; NF-L. Dysregulation of NEFL has been implicated in neurological function and brain physiology, making it a relevant biomarker for investigational studies.
Quantitative measurement of NEFL enables researchers to characterize its abundance in Serum, Plasma, Cerebrospinal Fluid and correlate levels with experimental conditions. Studies investigating NEFL have contributed to the understanding of neurodegeneration, disease pathogenesis, and therapeutic target validation.
This sandwich ELISA kit provides a validated, reproducible format for NEFL quantification across a defined standard curve range of 15.6-1000pg/mL. The assay supports experimental workflows in Neuroscience Research research.
Kit Components
Each kit contains sufficient reagents for one 96-well plate (approximately 40 samples in duplicate with standard curve):
Pre-coated 96-well microplate (capture antibody), HRP-conjugated detection antibody, recombinant standard protein, sample/standard diluent, concentrated wash buffer, TMB substrate solution, stop solution, and product manual.
Validated Lot Colorimetric ReadoutPerformance Data
| Assay Type | Sandwich ELISA |
|---|---|
| Detection Range | 15.6-1000pg/mL |
| Sensitivity (LLOD) | 5.9pg/mL |
| Validated Sample Types | Serum, Plasma, Cerebrospinal Fluid |
| Detection Method | Colorimetric (450 nm / 540–570 nm reference) |
| Plate Format | 96-well microplate |
| UniProt Accession | P07196 |
| NCBI Gene ID | 4747 |
| Shelf Life | Traditional: 12 months | Ready-to-use: 16 months |
Assay Procedure Summary
Add samples and standards to pre-coated capture antibody wells and incubate. Wash, add HRP-conjugated detection antibody, and incubate. Wash again, add TMB substrate, and stop the reaction. Read absorbance at 450 nm (reference 540–570 nm). Interpolate sample concentrations from the standard curve using a 4-parameter logistic (4-PL) fit.
Pre-warm all kit components to room temperature (18–25 °C) for 30 minutes before starting. Run all samples in duplicate. Centrifuge samples at ≥1,000 × g before use to remove particulates.
Safety & Intended Use
Intended UseResearch Use Only (RUO)VendorDLdevelopCatalog No.DL-NEFL-Hu, DLR-NEFL-HuSpeciesHumanUniProtP07196
This kit is validated for serum and plasma. Centrifuge samples at ≥1,000 × g for 15 min to remove particulates. Avoid hemolyzed, lipemic, or heat-inactivated samples. Pre-dilute samples within the linear range before committing full plates.
Minimum detectable concentration: 5.9pg/mL. Quantitative standard curve range: 15.6-1000pg/mL. Samples above the upper limit must be diluted; near-lower-limit samples require duplicate measurement.
A typical sandwich ELISA protocol for this kit requires 3.5–5 hours. Pre-warm reagents to room temperature (18–25 °C) for at least 30 minutes before starting and prepare standard dilutions while the capture plate equilibrates.
A standard microplate spectrophotometer set to 450 nm (reference: 540–570 nm) is required. An automated plate washer reduces wash-step variability. Calibrate and warm up your reader before measuring. Blank using the zero-standard or buffer-only well before constructing the standard curve.
Store the unopened kit at 2–8 °C until the expiry date. Shelf life: Traditional: 12 months | Ready-to-use: 16 months from date of manufacture. Reconstituted standard is stable at 4 °C for ≤1 week; prepare fresh for critical experiments.
Looking for a custom format, bulk quantity, or specialized formulation? We offer flexible sourcing options. Contact us to discuss your specific requirements.
