Human Osteoblasts-Rheumatoid Arthritis (HOb-RA)

Overview

Human Osteoblasts-Rheumatoid Arthritis (HOb-RA) is a cell model used for research applications where physiologically relevant identity and donor background support interpretation of experimental readouts. Human osteoblast derived from Bone (Osteoblasts-Rheumatoid Arthritis) associated with rheumatoid arthritis (RA) within the Musculoskeletal system.

Bone is a dynamic tissue, being continuously remodeled by the coordinated actions of osteoclasts and osteoblasts. Osteoblasts, the bone-forming cells, are derived originally from pluripotent mesenchymal stem cells. They synthesize and secrete organic extracellular matrix, osteoid, which is composed primarily of type I collagen. Osteoid is calcified by osteoblasts and during this process the cells become encased in lacunae within the calcified material and become osteocytes. Osteoblasts express protease-activated receptor-1 and vascular endothelial cell growth factor [1]. Studies show that leukemia inhibitory factor can bind to the osteoblast cell surface and induce bone formation both in vitro and in vivo [2]. The balance between osteoblast recruitment, proliferation, differentiation and apoptosis in sutures between cranial bones is essential for calvarial bone formation [3]. iXCells Biotechnologies provides high quality Human Osteoblasts-Rheumatoid Arthritis (HOb-RA), which are isolated from bone of patients with rheumatoid arthritis (RA) and cryopreserved at P2, with >0.5 million cells in each vial. RA provide an excellent model system to study the patophisiology of that disease in vitro, including matrix mineralization, effects of inflammatory mediators and bone morphogenic proteins, physiological control of bone remodeling and regulation of bone metabolism. HOb-RA are characterized by the cytochemical detection of AP and mineral deposition. These HOb-RA are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi and can further expand for 10 population doublings in Osteoblast Growth Medium (Cat# MD-0054) under the condition suggested by iXCells Biotechnologies.

Key elements and design rationale

• Cell identity: osteoblast (Primary Cells, Custom Cells)
• Source context: Bone; Osteoblasts-Rheumatoid Arthritis; Musculoskeletal
• Donor background: Disease/condition: rheumatoid arthritis (RA)
• Biosafety level: BSL-2 (follow your institution’s biosafety program and local regulations)

Product-specific elements (such as tissue source, donor background, and cell classification) help frame how results should be interpreted across assays and experimental conditions.

Biological background

Cells originating from the Musculoskeletal system are commonly studied to understand tissue-specific physiology, signaling, and responses to perturbations in controlled in vitro settings.

Across primary and specialty cell models, experimental outcomes can be influenced by donor heterogeneity, passage history, confluence, and media composition. For interpretation, it is common to validate key markers or functional phenotypes in the user’s assay context and to document culture variables consistently.

Research relevance and current trends

• Increasing use of primary and specialty cells to improve translational relevance for target biology and phenotypic screening.
• Adoption of 3D culture formats and co-culture systems to better capture tissue microenvironments and cell–cell interactions.
• Integration of functional readouts with single-cell and multi-omics profiling to connect phenotype with molecular state.

Common research applications

• Profile identity markers by flow cytometry or immunostaining in cultured cells
• Quantify functional responses to defined stimuli relevant to the model system
• Compare baseline phenotype across donors/conditions using gene expression profiling
• Evaluate matrix remodeling and differentiation programs in musculoskeletal cell models
• Model disease-associated phenotypes and compare responses to matched controls (assay dependent)

Interpretation typically focuses on how a perturbation (e.g., cytokine exposure, metabolic stress, genetic manipulation, or compound treatment) shifts marker profiles or functional readouts relative to an appropriate control matched for donor and culture variables.

Notes for experimental interpretation

• Donor-to-donor heterogeneity can influence baseline phenotype and treatment response; include biological replicates when feasible.
• Passage number, confluence, and media composition can shift gene expression and functional readouts; track and report these variables consistently.
• Contamination control (including routine mycoplasma monitoring) supports reproducibility in downstream assays.
• Use appropriate negative/positive controls for the readout (e.g., unstimulated controls, pathway agonists/antagonists) to contextualize observed changes.

SKU:BHC18500258

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