| Field | Specification |
|---|---|
| Target | |
| Alternative names | Plasminogen activator inhibitor 1;PAI;PAI-1;Endothelial plasminogen activator inhibitor;Serpin E1;SERPINE1;PAI1, PLANH1; |
| Gene ID | |
| UniProt # | |
| Reactivity | |
| Applications | |
| Immunogen | Expression system for standard: NS0; Immunogen sequence: S22-P402 |
| Expression system | |
| Expression region | |
| Assay type | |
| Sample type(s) | Cell Culture Supernatant, Cell Lysate, Serum, Plasma |
| Sensitivity | |
| Detection range | |
| Assay time | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Assay Principle
This sandwich ELISA quantifies human plasminogen activator inhibitor 1 (gene SERPINE1) in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA, citrate ). Strips are pre-coated with a capture antibody. After the sample is added, a biotinylated detection antibody binds the captured protein, and an avidin–biotin–peroxidase complex supplies the enzyme. TMB develops color in proportion to the bound target; the reaction is stopped and read at 450 nm, and concentrations are read from the standard curve.
Plasminogen activator inhibitor 1 (PAI-1, SERPINE1) is the main inhibitor of tissue-type and urokinase-type plasminogen activators and so acts as a brake on fibrinolysis. It also influences cell adhesion and migration and is strongly induced by TGF-β and other stimuli, making it a common target in studies of thrombosis, fibrosis, metabolism and cancer.
Kit Components
| Anti-Human SERPINE1 Pre-coated 96-well strip microplate | 1 plate (96 wells) |
|---|---|
| Human SERPINE1 Standard | 2 vials, 10 ng/tube |
| Human SERPINE1 Biotinylated antibody (100x) | 100 µL |
| Avidin-Biotin-Peroxidase Complex (100x) | 100 µL |
| Sample Diluent | 30 mL |
| Antibody Diluent | 12 mL |
| Avidin-Biotin-Peroxidase Diluent | 12 mL |
| Color Developing Reagent (TMB) | 10 mL |
| Stop Solution | 10 mL |
| Wash Buffer (25x) | 20 mL |
| Adhesive plate sealers | 4 |
Also required (not supplied): microplate reader for 450 nm; incubator; automated plate washer (optional); precision pipettes for 0.5 µL through 1 mL volumes of aqueous solutions; multichannel pipettes (recommended for many samples); deionized or distilled water; 500 mL graduated cylinders; test tubes for dilution.
Kit components are not sold separately.
Performance Data
| Species | Human |
|---|---|
| Sample types | Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA, citrate ) |
| Assay range | 156 pg/mL–10,000 pg/mL |
| Sensitivity | <10 pg/mL |
| Standard | Recombinant human SERPINE1 (Ser22–Pro402), expressed in NS0 |
| Cross-reactivity | None detected against related proteins in supplier testing |
| Intra-assay CV | 6.1–7.7% (3 samples, n = 16 each) |
| Inter-assay CV | 7.6–8.7% (3 samples, n = 24 each) |
| Format | 96 wells, removable strips |
Sample dilution: start at 1:1. In the supplier’s tests, serum and plasma samples measured 0.59–22.44 ng/mL.
Example standard curve (OD450, pg/mL): 0 = 0.055; 156 = 0.101; 312 = 0.138; 625 = 0.221; 1250 = 0.406; 2500 = 0.712; 5000 = 1.466; 10000 = 2.268. Run a new standard curve on every plate.
Storage & Handling
Store at 4 °C for up to 6 months or −20 °C for up to 12 months. Avoid repeated freeze–thaw cycles. Ships on gel ice and tolerates up to 3 days at room temperature; freeze on arrival.
Safety
Sample data
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.055 | 0.101 | 0.138 | 0.221 | 0.406 | 0.712 | 1.466 | 2.268 |
Intra/inter assay consistency
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 203 | 1208 | 5496 | 198 | 1244 | 5980 |
| Standard deviation | 12.38 | 71.27 | 423.19 | 15.04 | 95.78 | 520.26 |
| CV (%) | 6.1% | 7.7%% | 7.7% | 7.6% | 7.7% | 8.7% |
Kit components
Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4Materials required but not provided
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Bai Qin et al. (2025) Hexokinase 2-mediated histone H3K18la promotes PAI-1-dependent thrombosis in acute myeloid leukemia via tumor-endothelial crosstalk. Nature Communications. 10.1038/s41467-025-65259-0
Xinxin Zhang et al. (2021) Targeting inflammation-associated AMPK//Mfn-2/MAPKs signaling pathways by baicalein exerts anti-atherosclerotic action. Phytotherapy Research. 10.1002/ptr.7149
Sun Yan et al. (2018) Primed atypical ductal hyperplasia-associated fibroblasts promote cell growth and polarity changes of transformed epithelium-like breast cancer MCF-7 cells via miR-200b/c-IKKβ signaling. Cell Death & Disease. 10.1038/s41419-017-0133-1
Nargesi Arash Aghajani et al. (2015) The lost correlation between heat shock protein 70 (HSPA1A) and plasminogen activator inhibitor-1 in patients with type 2 diabetes and albuminuria. Cell Stress & Chaperones. 10.1007/s12192-015-0634-8
Wei Wu et al. (2016) Disequilibrium of Blood Coagulation and Fibrinolytic System in Patients With Coronary Artery Ectasia. Medicine. 10.1097/MD.0000000000002779
Abolhasani Sakhavat et al. (2019) Evaluation of Serum Levels of Inflammation, Fibrinolysis and Oxidative Stress Markers in Coronary Artery Disease Prediction: A Cross-Sectional Study. Arquivos Brasileiros de Cardiologia. 10.5935/abc.20190159
Chang-Ye Hui et al. (2013) Oligodeoxyribonucleotides derived from salmon sperm DNA: An alternative to defibrotide. Biologicals. 10.1016/j.biologicals.2012.12.003
Yıldırım Malik Ejder et al. (2017) The Association of Plasminogen Activator Inhibitor Type 1 (PAI-1) Level and PAI-1 4G/5G Gene Polymorphism with the Formation and the Grade of Endometrial Cancer. Biochemical Genetics. 10.1007/s10528-017-9796-7
S. He et al. (2017) Cardiometabolic risks vary by weight status in pediatric kidney and liver transplant recipients: A cross-sectional, single-center study in the USA. Pediatric Transplantation. 10.1111/petr.12984
Esin Sogutlu Sari et al. (2014) The prevalence of 4G/5G polymorphism of plasminogen activator inhibitor-1 (PAI-1) gene in central serous chorioretinopathy and its association with plasma PAI-1 levels. Cutaneous and Ocular Toxicology. 10.3109/15569527.2013.854372
