| Field | Specification |
|---|---|
| Target | |
| Alternative names | Platelet-derived growth factor subunit B;PDGF subunit B;PDGF-2;Platelet-derived growth factor B chain;Platelet-derived growth factor beta polypeptide;Proto-oncogene c-Sis;Becaplermin;PDGFB;PDGF2, SIS; |
| Gene ID | |
| UniProt # | |
| Reactivity | |
| Applications | |
| Immunogen | Expression system for standard: E.coli; Immunogen sequence: (S87-T211) + (S82-T190) |
| Expression system | |
| Expression region | |
| Assay type | |
| Sample type(s) | Cell Culture Supernatant, Cell Lysate, Serum, Plasma |
| Sensitivity | |
| Detection range | |
| Assay time | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Assay Principle
This sandwich ELISA quantifies human platelet-derived growth factor subunit A (gene PDGFA) in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Strips are pre-coated with a capture antibody. After the sample is added, a biotinylated detection antibody binds the captured protein, and an avidin–biotin–peroxidase complex supplies the enzyme. TMB develops color in proportion to the bound target; the reaction is stopped and read at 450 nm, and concentrations are read from the standard curve.
PDGF-A is a polypeptide chain of platelet-derived growth factor that forms PDGF-AA homodimers or PDGF-AB heterodimers with PDGF-B. Acting mainly through PDGFRα, it stimulates proliferation, migration and survival of mesenchymal cells and is needed for development of lung alveoli, the gut, oligodendrocytes and Leydig cells. It is studied in development, wound healing and fibrosis.
Kit Components
| Anti-Human PDGF-AB Pre-coated 96-well strip microplate | 1 plate (96 wells) |
|---|---|
| Human PDGF-AB Standard | 2 vials, 10 ng/tube |
| Human PDGF-AB Biotinylated antibody (100x) | 100 µL |
| Avidin-Biotin-Peroxidase Complex (100x) | 100 µL |
| Sample Diluent | 30 mL |
| Antibody Diluent | 12 mL |
| Avidin-Biotin-Peroxidase Diluent | 12 mL |
| Color Developing Reagent (TMB) | 10 mL |
| Stop Solution | 10 mL |
| Wash Buffer (25x) | 20 mL |
| Adhesive plate sealers | 4 |
Also required (not supplied): microplate reader for 450 nm; incubator; automated plate washer (optional); precision pipettes for 0.5 µL through 1 mL volumes of aqueous solutions; multichannel pipettes (recommended for many samples); deionized or distilled water; 500 mL graduated cylinders; test tubes for dilution.
Kit components are not sold separately.
Performance Data
| Species | Human |
|---|---|
| Sample types | Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) |
| Assay range | 31.2 pg/mL–2,000 pg/mL |
| Sensitivity | <2 pg/mL |
| Standard | Recombinant human PDGFA ((S87-T211)), expressed in E. coli |
| Cross-reactivity | None detected against related proteins in supplier testing |
| Intra-assay CV | 5.7–6.6% (3 samples, n = 16 each) |
| Inter-assay CV | 7.2–7.9% (3 samples, n = 24 each) |
| Format | 96 wells, removable strips |
Example standard curve (OD450, pg/mL): 0 = 0.023; 31.2 = 0.067; 62.5 = 0.103; 125 = 0.187; 250 = 0.328; 500 = 0.634; 1000 = 1.146; 2000 = 2.02. Run a new standard curve on every plate.
Storage & Handling
Store at 4 °C for up to 6 months or −20 °C for up to 12 months. Avoid repeated freeze–thaw cycles. Ships on gel ice and tolerates up to 3 days at room temperature; freeze on arrival.
Safety
Sample data
| Concentration (pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
| O.D. | 0.023 | 0.067 | 0.103 | 0.187 | 0.328 | 0.634 | 1.146 | 2.02 |
Intra/inter assay consistency
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 60 | 259 | 808 | 62 | 271 | 865 |
| Standard deviation | 3.96 | 14.76 | 51.71 | 4.52 | 19.51 | 68.33 |
| CV (%) | 6.6% | 5.7% | 6.4% | 7.3% | 7.2% | 7.9% |
Kit components
Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4Materials required but not provided
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Liu Xiaoyao et al. (2022) Genipin modified lyophilized platelet-rich fibrin scaffold for sustained release of growth factors to promote bone regeneration. Frontiers in Physiology. 10.3389/fphys.2022.1007692
Limin Bai et al. (2023) Impact of a Novel Hydrogel with Injectable Platelet-Rich Fibrin in Diabetic Wound Healing. Journal of Diabetes Research. 10.1155/2023/7532637
Filippelli Mariaelena et al. (2021) Anti-inflammatory Effect of Curcumin, Homotaurine, and Vitamin D3 on Human Vitreous in Patients With Diabetic Retinopathy. Frontiers in Neurology. 10.3389/fneur.2020.592274
Quanguang Ren et al. (2019) Platelet-derived alpha-granules are associated with inflammation in patients with NK/T-cell lymphoma-associated hemophagocytic syndrome. Cytokine. 10.1016/j.cyto.2019.154878
Leonardo R. Rocha et al. (2017) Bone intramedullary reaming grafts the fracture site with CD146+ skeletal progenitors and downmodulates the inflammatory environment. Injury-International Journal of the Care of the Injured. 10.1016/S0020-1383(17)30774-X
Takeuchi Michiko et al. (2012) Human platelet-rich plasma promotes axon growth in brain–spinal cord coculture. Neuroreport. 10.1097/WNR.0b013e3283567196
Mubin Hosnuter et al. (2016) Functional assessment of autologous platelet-rich plasma (PRP) after long-term storage at −20 °C without any preservation agent. Journal of Plastic Surgery and Hand Surgery. 10.1080/2000656X.2016.1237956
