| Field | Specification |
|---|---|
| Target | |
| Alternative names | Platelet-derived growth factor subunit B; PDGF subunit B; PDGF-2; Platelet-derived growth factor B chain; Platelet-derived growth factor beta polypeptide; Proto-oncogene c-Sis; INN: Becaplermin; PDGFB; PDGF2; SIS |
| Gene ID | |
| UniProt # | |
| Reactivity | |
| Applications | |
| Immunogen | Expression system for standard: E.coli; Immunogen sequence: S82-T190 |
| Expression system | |
| Expression region | |
| Assay type | |
| Sample type(s) | Cell Culture Supernatant, Serum, Plasma |
| Sensitivity | |
| Detection range | |
| Assay time | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Assay Principle
This sandwich ELISA quantifies human platelet-derived growth factor subunit B (gene PDGFB) in cell culture supernatants, serum and plasma (heparin, EDTA). Strips are pre-coated with a capture antibody. After the sample is added, a biotinylated detection antibody binds the captured protein, and an avidin–biotin–peroxidase complex supplies the enzyme. TMB develops color in proportion to the bound target; the reaction is stopped and read at 450 nm, and concentrations are read from the standard curve.
PDGF-B is a growth factor that forms PDGF-BB homodimers or PDGF-AB heterodimers and signals through PDGF receptors. It is a strong mitogen for fibroblasts and smooth muscle cells, recruits pericytes to new blood vessels and contributes to wound healing.
Kit Components
| Anti-Human PDGF-BB Pre-coated 96-well strip microplate | 1 plate (96 wells) |
|---|---|
| Human PDGF-BB Standard | 2 vials, 10 ng/tube |
| Human PDGF-BB Biotinylated antibody (100x) | 100 µL |
| Avidin-Biotin-Peroxidase Complex (100x) | 100 µL |
| Sample Diluent | 30 mL |
| Antibody Diluent | 12 mL |
| Avidin-Biotin-Peroxidase Diluent | 12 mL |
| Color Developing Reagent (TMB) | 10 mL |
| Stop Solution | 10 mL |
| Wash Buffer (25x) | 20 mL |
| Adhesive plate sealers | 4 |
Also required (not supplied): microplate reader for 450 nm; incubator; automated plate washer (optional); precision pipettes for 0.5 µL through 1 mL volumes of aqueous solutions; multichannel pipettes (recommended for many samples); deionized or distilled water; 500 mL graduated cylinders; test tubes for dilution.
Kit components are not sold separately.
Performance Data
| Species | Human |
|---|---|
| Sample types | Cell culture supernatants, serum and plasma (heparin, EDTA) |
| Assay range | 31.2 pg/mL–2,000 pg/mL |
| Sensitivity | <10 pg/mL |
| Standard | Recombinant human PDGFB (Ser82–Thr190), expressed in E. coli |
| Cross-reactivity | None detected against related proteins in supplier testing |
| Intra-assay CV | 4.4–6.6% (3 samples, n = 16 each) |
| Inter-assay CV | 5.0–6.6% (3 samples, n = 24 each) |
| Format | 96 wells, removable strips |
Sample dilution: start at 1:1. In the supplier’s tests, serum samples measured about 1 ng/mL.
Example standard curve (OD450, pg/mL): 0 = 0.011; 31.2 = 0.071; 62.5 = 0.133; 125 = 0.247; 250 = 0.468; 500 = 0.866; 1000 = 1.581; 2000 = 2.505. Run a new standard curve on every plate.
Storage & Handling
Store at 4 °C for up to 6 months or −20 °C for up to 12 months. Avoid repeated freeze–thaw cycles. Ships on gel ice and tolerates up to 3 days at room temperature; freeze on arrival.
Safety
Sample data
| Concentration (pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
| O.D. | 0.011 | 0.071 | 0.133 | 0.247 | 0.468 | 0.866 | 1.581 | 2.505 |
Intra/inter assay consistency
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 94 | 199 | 788 | 88 | 194 | 725 |
| Standard deviation | 4.13 | 12.53 | 39.4 | 5.09 | 12.80 | 36.25 |
| CV (%) | 4.4% | 6.6% | 5% | 5.9% | 6.6% | 5% |
Kit components
Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4Materials required but not provided
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Dai Lei et al. (2016) SARI inhibits angiogenesis and tumour growth of human colon cancer through directly targeting ceruloplasmin. Nature Communications. 10.1038/ncomms11996
Fei Li et al. (2024) A 3D radially aligned nanofiber scaffold co-loaded with LL37 mimetic peptide and PDGF-BB for the management of infected chronic wounds. Materials Today Bio. 10.1016/j.mtbio.2024.101237
Zhang Jin et al. (2024) Adipose-derived stem cells derived decellularized extracellular matrix enabled skin regeneration and remodeling. Frontiers in Bioengineering and Biotechnology. 10.3389/fbioe.2024.1347995
Limin Bai et al. (2023) Impact of a Novel Hydrogel with Injectable Platelet-Rich Fibrin in Diabetic Wound Healing. Journal of Diabetes Research. 10.1155/2023/7532637
Quanguang Ren et al. (2019) Platelet-derived alpha-granules are associated with inflammation in patients with NK/T-cell lymphoma-associated hemophagocytic syndrome. Cytokine. 10.1016/j.cyto.2019.154878
López Jenny F. et al. (2018) Adipose tissue extract shows potential for wound healing: in vitro proliferation and migration of cell types contributing to wound healing in the presence of adipose tissue preparation and platelet rich plasma. Cytotechnology. 10.1007/s10616-018-0211-y
